53 results about "Curvularia" patented technology

The invention discloses bacillus subtilis A16, which is preserved in China Center for Type Culture Collection (CCTCC) with CCTCC NO. 209105. The bacterial colony of the Bacillus subtilis A16 is oyster white on a beef extract peptone culture medium, has irregular shape and no luster, is not wet, has irregular edge, has diameter of 0.5-1cm, and has microfold surface, straight rod-shaped thallus and elliptic spore. The invention also discloses a preparation method and application of the bacillus subtilis A16. The bacillus subtilis A16 has broad bacteriostasis, can suppress exserohilum turcicum and helminthosporium maydis, has higher antagonism for exserohilum turcicum bacteria, helminthosporium maydis bacteria, maize curvularia leaf spot fungus, verticillium dahliae kleb, wheat scab pathogen, colletotrichum musae and banana vascular wilt bacteria, and has high prevention cure efficiency, and good safety and development application prospect.

The present invention is directed to methods and compositions of endophytic fungi that confer stress tolerance in inoculated plants, including both monocots and dicots. In particular, Curvularia species, isolated from a host grass Dichanthelium languinosum growing in the geothermal zones of Lassen Volcanic and Yellowstone National Parks, confers such stress tolerance. Upon inoculating a target plant or plant part with endophytic fungi, the resulting plant shows stress tolerance, particularly drought and thermal tolerance.

The invention relates to a method for preparing biological weed killer, wherein it uses Curvularia Boedijn Mds0404 CGMCC NO. 1827 as seed, which comprises 80-95% spore, 0-18% chemical weed killer, and 2-5% agent. And its production comprises that: activating seed, cultivating seed liquid, cultivating spore and collecting components. The invention has low cost and wide application.

The invention discloses bacillus licheniformis 201 for high effectively preventing banana wilt, and the preservation number of the bacillus licheniformis 201 is CCTCCM207092. Bacterial colony on beef extract-peptone medium is greyish-white, round, flat and lackluster, the edge of the bacterial colony is in order, the diameter of the bacterial colony is generally 2 to 3 millimeters, bacteria are of straight rod shape, the size of the bacteria is 2.06* 0.70 micrometers, and spore is of elliptical shape. Simultaneously, the invention also discloses a process for preparation and usage. The bacillus licheniformis 201 is capable of strongly preventing fusarium oxysporum and has wide antimicrobial spectrum, which is capable of performing antagonistic action on various pathogenic fungi, such as fusarium oxysporum, fusarium oxysporum f. sp. niveum, fusarium oxysporum f. sp. lycopersici, gloeosporium musarum, colletotrichum gloeosporioides, maize curvularia leaf spot fungus, verticillium wilt pathogen, wheat scab pathogen and the like, furthermore, the invention is high effective in prevention, good in environmental security and good in the prospect of development and application.

The invention discloses a Bacillus licheniformis 201, which is preserved in China Center for Type Culture Collection that is located in Wuhan University, Logosun, Wuchang, Wuhan, Hubei, and with CCTCC NO. of M207092. The invention simultaneously discloses a preparation method and applications of the Bacillus licheniformis 201, which has strong bacteriostasis of banana wilt fusarium, wide bacteriostasis spectrum and antagonism to various pathogenic fungi including banana wilt fusarium, watermelon wilt fusarium, tomato wilt fusarium, banana colletotrichum, gloeocystidium colletotrichum, corn leaf spot curvularia, cotton verticillium lecanii, gibberella zeae, and the like, and the Bacillus licheniformis 201 has high control efficiency, good environmental security and good development and application prospect.

The invention relates to the technical field of a medicine, and particularly relates to two diterpenoid compounds libertellenone G and libertellenone H with antineoplastic activities extracted from secondary metabolite of arctic curvularia molecular genus. The chemical structural formulas are shown in the specification. The invention also provides a preparation method of the diterpenoid compounds, and application of the diterpenoid compounds in preparation of an antitumor drug.

The invention provides bacillus siamensis GL-02, the preservation number of which is CGMCC No.16068. A single colony formed by the strain propagating and growing on a beef extract peptone (NB) culturemedium is round, milky white, slightly bulgy in center, and moist and transparent on surface; through microscopic examination, the single colony is rod-shaped, flagellated, 0.7-0.8*1.6-2.2[mu]m in size, and G+,with spores. The single colony can grow on a beef extract peptone culture medium containing 2-5% NaCl. The formula of the beef extract peptone (NB) culture medium comprises 3g of beef extract, 10g of peptone, 12g of agar, and 1L of water, and the pH value is 6.8-7.0. The bacillus siamensis GL-02 has a significant antagonistic effect on pathogens causing corn stalk rot, bipolaris maydisand curvularia leaf spot. The invention also provides application of bacillus siamensis GL-02 in controlling plant fungal diseases, or application in preparing microbial preparations for controlling plant fungal diseases.

The invention relates to a liquid state composite biological multivariable control paddy herbicide. The herbicide is characterized by being prepared from medicinal plants and multiple species inoculant. The medicinal plants comprise, by weight, 5-25 parts of radix euphorbiae lantu, 5-25 parts of cumin, 5-25 parts of garlic, 5-25 parts of radix angelicae pubescentis and 5-25 parts of common cnidium fruits. The multiple species inoculant comprises, by weight, 5-20 parts of bacterial liquid of streptomyces lavendulae, 5-20 parts of bacterial liquid of streptomyces fradiae, 5-20 parts of bacterial liquid of streptomyces viridocbromogenes, 5-20 parts of bacterial liquid of streptomyces griseolus, 5-20 parts of bacterial liquid of alternaria, 5-20 parts of bacterial liquid of botryis cinerea and 5-20 parts of bacterial liquid of curvuloria lunata. The biological multivariable control paddy herbicide is efficient, low in toxicity, environmentally friendly and not likely to produce resistance and particularly has the remarkable restraining effect on barnyard grass, nutgrass flatsedge, semen euphorbiae and duckweed.

The invention relates to a polymerase chain reaction (PCR) detection kit for maize curvularia leaf spot and a detection method for the PCR detection kit. The PCR detection kit for the maize curvularia leaf spot comprises two PCR reaction systems; the first reaction system comprises a first primer pair consisting of two specific primers shown as SEQIDNO:1 and SEQIDNO:3; and the second reaction system comprises a second primer pair consisting of two specific primers shown as SEQIDNO:2 and SEQIDNO:3. The specific amplification primers are designed according to clg2p gene of the maize curvularia leaf spot, and the PCR detection kit for the maize curvularia leaf spot is constructed and applied to molecular detection of the maize curvularia leaf spot, can accurately and quickly perform molecular identification on maize curvularia leaf spot pathogenic bacteria, also can be used for detecting whether diseased maize seedlings having symptoms such as leaf spot and death are caused by the maize curvularia leaf spot, and has a great application prospect for identifying the maize curvularia leaf spot pathogenic bacteria and monitoring diseases in field.

The invention relates to a method for genetic transformation of germinating conidiophore of Curvularia lunata Boed. The method comprises the following steps: culturing the germinating conidiophore of the Curvularia lunata Boed as a receptor by a slide suspension method; co-culturing agrobacterium containing an epiphyte conversion vector system and the germinating conidiophore; and screening transformants with resistibility on an antibiotic culture medium. The system uses the Curvularia lunata Boed as initial bacteria, spore is scratched by 0.9 percent of physiological saline after the Curvularia lunata Boed is cultured on the PDA culture medium for 5 to 7 days, and is used for infection of the agrobacterium after the spore is germinated by the slide suspension culture method. The infected germinating conidiophore obtains resistant transformant by co-culture, bacteriostasis culture and anti-selective agent screening culture of continuous two generations, so that a Curvularia lunata Boed mutant base is constructed to provide rich screening strain sources for weak pathogenicity and pathogenic defective Curvularia strain, and meanwhile can be used for cloning related gene of Curvularia lunata Boed pathogenicity so as to lay a foundation for further investigating the pathogenesis mechanism of the Curvularia lunata Boed strain.

The invention relates to a method for constructing a mutant strain of maize curvularia leaf spot fungus. The curvularia lunata strain is picked up to be subject to the intermediate culture to prepare the protoplast suspending liquid; colon bacillus strain in cold-preservation containing plasmids is picked up to be subject to the inoculation culture to be extracted plasmid DNA and prepare the enzyme-plasmid mixing liquid; the protoplast suspending liquid and the enzyme-plasmid mixing liquid are evenly mixed and subject to the culture and the filtration to obtain mutant, and the mutant is cultured on PDAs with hygromycin and without hygromycin, and is filtered to obtain the stable mutant strain of maize curvularia leaf spot fungus. The method adopts the restriction Enzyme-mediated gene integration technology to mix the plasmid DNA and the prepared trichoderma protoplast and makes the exogenous linear plasmid fragments inserted in the genome to induce the insertion mutation under the action of the restriction Enzyme-mediated. The mutant strain obtained by the method provides abundant filtrating bacteria sources for the filtration of the weak pathogenic curvularia lunata strain, and can be used to the cloning of genes related to the maize curvularia lunata pathogenicity and the research on the pathogenesis mechanism of the maize curvularia lunata strain.

The invention provides noni endophytic bacillus siamensis, which is named as bacillus siamensis QN2MO-1, is stored in the China Center for Type Culture Collection (CCTCC), and has the preservation number of CCTCC M 2021305. The strain has stable broad-spectrum antagonistic activity; and strong antibacterial effect on tomato fusarium wilt, banana fusarium wilt, cucumber fusarium wilt, pepper colletotrichum gloeosporioides, strawberry colletotrichum gloeosporioides, banana colletotrichum gloeosporioides, cabbage colletotrichum gloeosporioides, corn curvularia leaf spot, mango leaf blight, wheat scab, cryphonectria parasitica, mango pestalotiopsis leaf blight, rice blast, and colletotrichum gloeosporioides; its fermentation broth can effectively inhibit the infection of pathogen filaments on in-vitro leaves, fruits and the like, increases the safe sources of the effective components of the biocontrol agent, expands a new field for the prevention and treatment of tomato fusarium wilt and other plant diseases, and has good development and application prospects.

The invention relates to a trichoderma phospholipase A2 and a gene for expressing the same. The invention belongs to the field of agricultural biotechnology. The invention provides sequences of the trichoderma phospholipase A2 (PLA2) amino acid and nucleotide, and constructs efficient prokaryotic expression of the trichoderma phospholipase A2 and purifies system; the invention constructs deletion mutants of the gene, and uses the gene to prevent and control the curvularia leaf spot and achieves good effect. The invention firstly gains the trichoderma phospholipase A2 from filamentous fungi, and clones the gene which expresses the same. The efficient method for gaining the trichoderma phospholipase A2 provides the possibility of producing enzyme preparation. The trichoderma phospholipase A2 has double functions of both esterase and phospholipase A2, which indicates the trichoderma phospholipase A2 has good application value in industrial catalysis; and the function of the deletion mutants of the gene on preventing and controlling the curvularia leaf spot provides new application, and can greatly reduce curvularia leaf spot disease to improve maize yield.

The invention relates to a molecular detection primer for banana cladosporium cucumerinum and a detection method and belongs to the technical field of crop disease detection and biotechnology. The specific primer comprises an upstream primer BMF: 5'-GCTACAACGCCGAAATGACC-3' and a downstream primer BMR: 5'-GACGTTGCCCAATACCAAGC-3'. The detection primer and the detection method provided by the invention can be used for detection and identification of banana cladosporium cucumerinum pure culture and also can be used for detecting the banana leaves and fruit; the detection primer provided by the invention is high in specificity and sensitivity; the detection method is high in practicability and simple and convenient in operation; the molecular detection primer can be used for realizing the early detection for banana cladosporium cucumerinum and effectively identifying the similar anthracnose, curvularia disease, black spot, spore leaf spot disease and dark agaricus leaf spot disease of banana, early warning, preventing and controlling the banana cladosporium cucumerinum; the molecular detection primer has significance in preventing and treating disease spreading.

This invention is intended to identify a gene cluster involved in biosynthesis of a cyclic peptide compound produced by a filamentous fungus of the Curvularia species and to establish a system for synthesizing such cyclic peptide compound. The gene is composed of a first module to a tenth module and encodes a protein having activity of synthesizing a nonribosomal peptide constituting a basic peptide backbone of a cyclic peptide compound produced by a filamentous fungus of the Curvularia species.

The invention discloses a biological herbicide and a preparation method thereof, belonging to the technical field of using microorganisms to control farmland weeds. The herbicide takes Curvularia Boedijn (Curvularia Boedijn) Mds0404 strain CGMCCNO.1827 as the bacterial species, and the weight percentage content of its components and each component is: 80-95% of pure spores, 0-18% of chemical herbicides, and auxiliary agents 2-5%; prepared by the preparation of the culture medium, the activation of the strain Mds0404, the cultivation of the seed solution, the production, cultivation and collection of the spores of the strain, and the mixing of various components. The agent mainly controls crabgrass, and also kills foxtail, barnyardgrass, goosegrass and other weeds. It is safe for crops, especially for monocot crops, has wide application adaptability, low cost, and no pollution. It can be popularized and applied in crabgrass crabgrass and other weed-infested areas.

The invention belongs to the field of crop breeding for disease resistance and relates to an early identification method for curvularia leaf spot in maize inbred lines. The early identification method for curvularia leaf spot in maize inbred lines includes treating leaves of three-leaf seedlings of maize inbred lines in maize curvularia lunata toxin solution, and mixing with sodium borate buffer solution to extract enzyme crude extract of phenylalnine ammonialyase, peroxidase and superoxide dismutase in the leaves; measuring enzyme activities of the phenylalnine ammonialyase, the peroxidase and the superoxide dismutase, and calculating specific activity; calculating resistance values according to a formula, and calculating average of the resistance values. The average larger than 3 is the value of resistance of the inbred line to maize curvularia leaf spot. The method is accurate and reliable. The method can be used to identify and screen indoors without limitations of external environment conditions. In addition, the method has no season limitation in seedling-stage disease resistance identification, and shortens identification time. The method is high in work efficiency.

The invention discloses an application of itraconazole in the preparation of fungicides for the prevention and control of plant pathogenic bacteria. Experiments prove herein that the itraconazole hasgood inhibitory activity against the main diseases of important grain crops, including fusarium graminearum causing wheat scab, magnaporthe oryzae causing rice blast, rhizoctonia cerealis causing sheath blight and curvularia maize causing maize leaf spot; moreover, the itraconazole has good inhibitory activity against the physalospora piricola causing apple ring rot, valsa mali causing apple rot and alternaria tenuifolia causing leaf spot disease of fruits and vegetables; the itraconazole also has good inhibitory activity against soil-borne diseases that are extremely difficult to prevent andcontrol in agricultural production, such as fusarium oxysporum causing blight, and the inhibitory activity is even better than those of traditional fungicides such as carbendazim and tebuconazole. Forthe first time, the itraconazole, a green small-molecular compound screened through three-dimensional structures of target proteins, is applied to the prevention and control of important plant diseases in agricultural production and has a good market application prospect.

The invention belongs to the technical field of microorganism culture, and particularly relates to a cleavers-containing curvularia culture medium and a preparation method thereof.According to a formula for preparing 1000 ml of the culture medium, 20-160 g of cleavers, 1.0 g of peptone, 1.0 g of glucose, 0.5 g of magnesium sulfate heptahydrate, 0.5 g of monopotassium phosphate and 15-20 g of agar are taken, distilled water is added till the volume is 1000 ml, and the pH is regulated to be 6.5-8.0.By means of the cleavers-containing curvularia culture medium and the preparation method thereof, the curvularia culture cost is reduced; by adding common field grass into a culture medium, the fine culture medium widely applied to curvularia is developed.

The invention discloses bacillus subtilis A16, which is preserved in China Center for Type Culture Collection (CCTCC) with CCTCC NO. 209105. The bacterial colony of the Bacillus subtilis A16 is oyster white on a beef extract peptone culture medium, has irregular shape and no luster, is not wet, has irregular edge, has diameter of 0.5-1cm, and has microfold surface, straight rod-shaped thallus andelliptic spore. The invention also discloses a preparation method and application of the bacillus subtilis A16. The bacillus subtilis A16 has broad bacteriostasis, can suppress exserohilum turcicum and helminthosporium maydis, has higher antagonism for exserohilum turcicum bacteria, helminthosporium maydis bacteria, maize curvularia leaf spot fungus, verticillium dahliae kleb, wheat scab pathogen, colletotrichum musae and banana vascular wilt bacteria, and has high prevention cure efficiency, and good safety and development application prospect.

The invention relates to a compound with simple structure for inhibiting growth activity of phytopathogens, specifically relates to a 3-coumarin formic acid compound and application of the 3-coumarin formic acid compound as an antibacterial agent for preparing the phytopathogens, and discloses application of the 3-coumarin formic acid compound as the antibacterial agent for preparing the phytopathogens. The two 3-coumarin formic acid compounds has the following characteristics of molecular structure: the formulas are as shown in the specification, wherein R is hydrogen, methyl, methoxyl, hydroxyl, halogen and nitryl. The in vitro inhibitory activity of twenty-eight synthetic target compounds on four common plant pathogenic fungi such as apple decay pathogenic bacteria, apple ring spot pathogenic bacteria, maize curvularia pathogenic bacteria and potato dry rot pathogenic bacteria is measured by adopting a hypha linear growth rate method, so that the condition that all compounds have different inhibiting effects on supplied experimental bacteria is found.

The invention discloses application of posaconazole to preparation of a bactericide for controlling plant pathogenic bacteria. Determination of indoor drug sensitivity proves that the posaconazole hasgood activity in inhibiting main diseases of important food crops, including wheat scab-causing fusarium graminearum, rice blast-causing magnaporthe oryzae, sheath blight-causing rhizoctonia cerealisand corn leaf spot-causing maize curvularia, has good activity in inhibiting botrytis cinerea, physalospora piricola, valsa ceratosperma and alternaria tenuissima, and has relatively good activity ininhibiting soil-borne diseases such as fusarium oxysporum, which are extremely difficult to control in agricultural production, and the bacteriostatic activity of the posaconazole is even superior tothose of the conventional bactericides carbendazim and tebuconazole. The invention proposes screening of the green small molecular compound posaconazole from the three-dimensional structure of targetprotein for the first time, and the posaconazole is applied to control of important plant diseases in the agricultural production and has a broad market application prospect.

The invention relates to the technical field of low temperature damage resistance, in particular to the application of a paclobutrazol.pyraclostrobin pesticide composition in preparing a crop low temperature damage resisting chemical regulator. The pesticide composition in the application is prepared from paclobutrazol and pyraclostrobin, wherein the mass ratio of the paclobutrazol to the pyraclostrobin is 1-2 to 1-10. Compared with a conventional single-purpose chemical regulator, a paclobutrazol.pyraclostrobin suspended seed coating disclosed by the invention has good regulatory effects on low temperature damage and also has better prevention and control effects on the important disease of curvularia leaf spot; in addition, the regulatory effects of a mixture of the paclobutrazol and thepyraclostrobin on low temperature damage resistance and disease resistance are significantly higher than those of single paclobutrazol or single pyraclostrobin; the paclobutrazol.pyraclostrobin pesticide composition has the characteristics of one drug with multiple control effects, so that the use frequency and the use amount of pesticides can be reduced and further the production cost is reduced.

The invention relates to a molecular detection primer for banana cladosporium cucumerinum and a detection method and belongs to the technical field of crop disease detection and biotechnology. The specific primer comprises an upstream primer BMF: 5'-GCTACAACGCCGAAATGACC-3' and a downstream primer BMR: 5'-GACGTTGCCCAATACCAAGC-3'. The detection primer and the detection method provided by the invention can be used for detection and identification of banana cladosporium cucumerinum pure culture and also can be used for detecting the banana leaves and fruit; the detection primer provided by the invention is high in specificity and sensitivity; the detection method is high in practicability and simple and convenient in operation; the molecular detection primer can be used for realizing the early detection for banana cladosporium cucumerinum and effectively identifying the similar anthracnose, curvularia disease, black spot, spore leaf spot disease and dark agaricus leaf spot disease of banana, early warning, preventing and controlling the banana cladosporium cucumerinum; the molecular detection primer has significance in preventing and treating disease spreading.

The invention discloses application of voriconazole to preparation of a bactericide for controlling phytopathogens. Measurement on indoor drug sensitivity proves that the voriconazole has high inhibitory activity on main diseases of important grain crops, including fusariumgraminearum and maize curvularia; moreover, the voriconazole has high inhibitory activity on macrophomakawatsukai causing apple ring rot, valsaceratosperma causing apple rot and alternariatenuissima causing leaf spot of fruits and vegetables; furthermore, the voriconazole has relatively high inhibitory activity on soil-bornediseases extremely difficultly controlled in agricultural production, such as fusariumoxysporum, and the bacteriostatic activity thereof is even higher than that of the conventional bactericides suchas carbendazim and tebuconazole. The problem of a poor control effect caused by resistance of the plant pathogenic fungi such as the fusariumgraminearum and the macrophomakawatsukai to the existing bactericides can be effectively solved; and the voriconazole is environment-friendly and pollution-free, meets residual drug toxicity standards of the World Health Organization, and has a broad marketapplication prospect.

The invention discloses a steroid 11β-hydroxylase from Curvularia lunae, its coding gene and application. The steroid 11β-hydroxylase provided by the present invention is the protein shown in SEQ ID No.1 (ie CL3-CYP021 protein). In addition, the present invention also provides a complete set of proteins consisting of steroid 11β-hydroxylase and the protein shown in SEQ ID No.2 (ie CL3-CPR protein). The present invention expresses CL3-CYP021 protein (or CL3-CYP021 and CL3-CPR protein) in heterologous microorganisms to catalyze the synthesis of hydrocortisone (HC) and 14α-hydroxycortisol. The present invention replaces the original filamentous fungus with yeast for biocatalytic fermentation to produce HC, which has the advantages of shortening fermentation time, simplifying fermentation conditions and separation and extraction steps, and reducing production costs, and is important for replacing traditional hydrocortisone biocatalytic fermentation production methods significance.

The invention discloses a corn hybridization method, and relates to the technical field of agricultural planting. The corn hybridization method comprises parent selfing line breeding, a hybridization planting mode, a parent breeding mode and cultivation management, wherein the parent selfing line breeding comprises a female parent selfing line L297 and a male parent selfing line L91, the female parent selfing line L297 is obtained by hybridizing a selfing line L216 and a selfing line L117 and then carrying out continuous multi-generation selfing separation according to a pedigree method, and a new corn inbred line with stable and uniform plant morphology and agronomic traits is selected. By means of the hybridization mode, high-quality Wanyu 17 is cultivated, so that corn is high and stable in yield, high in reverse folding resistance, high in seed production rate, wide in adaptability, high in volume weight and high in starch content; and by integrating disease resistance identification results of two years, the variety can resist (MR) fusarium stem rot, southern leaf blight, fusarium ear rot, curvularia leaf spot, gall smut and southern rust disease, so that the disease resistance is better.

The invention relates to application of a 1-sulfonyl naphthol derivative in prevention and treatment of plant pathogenic fungi and an antibacterial agent, and belongs to the technical field of antibacterial agents. In the application of the 1-sulfonyl naphthol derivative, the 1-sulfonyl naphthol derivative has a structure shown as a formula I, and in the formula I, R is C1-C6 alkyl, aryl or heteroaryl, wherein the aryl is phenyl, substituent substituted phenyl or naphthyl. In the application of the 1-sulfonyl naphthol derivative of the present invention, the 1-sulfonyl naphthol derivative canbe used for detecting 16 plant pathogenic fungi such as fusarium graminearum, wheat root rot fungi, wheat stem rot fungi, magnaporthe oryzae, setosphaeria turcica, bipolaris maydis, maize curvularia leaf spot, oilseed rape sclerotinia sclerotiorum, tobacco fusarium oxysporum, phytophthora nicotianae, alternaria alternata, cotton fusarium oxysporum, cucumber fusarium oxysporum and botrytis cina, alternaria brassicae and phytophthora capsici, and shows good antibacterial activity.