The invention relates to an
agrobacterium rhizogenes mediated spraying decoration transgenosis method. In the method, a process that the
agrobacterium rhizogenes suspension is directly sprayed on the
stamen before
pollen-shedding stage and the
stamen in the
pollen-shedding stage under the condition of
living body in the field environment is adopted, thus having simpler operation and avoiding the damage to the organ of flower at the same time. Calculated as the number of the transgenosis
plant / transforming flower, the
transformation efficiency in the cotton can be 10%. The method of the invention has great practical application value in the
plant genetic transformation and
molecular breeding. The transforming material used in the invention can be Malvaceae cotton, or monocotyledonous and dicotyledoneae
seedling plants such as rice, corn, wheat, soybean,
rapeseed plant, cucumber, tomato, tobacco, fruit trees, forest, flowers and
officinal plants and the like, and has a certain application value in the genetic transformation and
molecular breeding of the monocotyledonous and dicotyledoneae
seedling plants.