Reusable transgenic arabidopsis water culture device and water culture method thereof

Abstract

The invention discloses a reusable transgenic arabidopsis water culture device and a water culture method thereof and belongs to the technical field of water culture of transgenic arabidopsis screened homozygous lines. The device comprises an open container with the trapezoidal longitudinal section, a supporting panel and a preservative film cover. The supporting panel is formed by sewing a black KT plate and a stainless steel net together through a nylon fish wire, small holes are formed in the middle of the black KT plate, and the holes are filled with sterile vermiculite. The opening portion of the container is sealed by the preservative film cover. Transgenic arabidopsis which has certain resistance and meets the Mendelian inheritance rule is screened out, taken out with a disinfected small spoon, transferred to the vermiculite in the small holes of the black KT plate and sealed by the preservative film for water culture. The device can be reused after being manufactured at a time, and is simple in structure and convenient to operate. Through simple nutrient solution water level control, transgenic arabidopsis transplanted seedlings are high in survival rate, rapid in pot adaptation and early in maturation, and meanwhile the problems that during transgenic arabidopsis water culture, lodging, algae growing and bacterium growing are likely to happen are solved.

Description

technical field [0001] The invention belongs to the technical field of plant cultivation, in particular to a recyclable transgenic Arabidopsis homozygous strain screening rapid propagation hydroponic device and a hydroponic method thereof. Background technique [0002] Arabidopsis is an important model plant for studying plant gene functions because of its short growth cycle, self-pollination, multiple species, small genome, high gene homozygosity, and genome sequencing has been completed. With the development of molecular biology, the verification of various gene functions, gene transcription and regulation, and proteomics research are widely used in transgenic Arabidopsis. In plant transgenic technology, Agrobacterium-mediated transformation is widely used, and Arabidopsis is the most widely used carrier for studying plant gene functions. In transgenic Arabidopsis research, it is generally required to obtain a large number of homozygous T 3 Generation lines, and then usi...

AI Technical Summary

Problems solved by technology

At the same time, the Arabidopsis hydroponics method is in the artificial greenhouse, and the nutrient solution is irradiated by light, which is easy to grow algae and fungi, which affects the growth of Arabidopsis and even dies; therefore, there is an urgent need for a transgene that can be recycled in the artificial greenhouse. Arabidopsis rapid propagation hydroponic device and hydroponic method thereof

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