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Economical and efficient production method of tobacco male sterile first generation hybrid seed

A male sterile and efficient technology, applied in botany equipment and methods, applications, plant genetic improvement, etc., can solve the problems of high production cost of hybrid seed production, no standard seed yield, and lack of pollinators, so as to eliminate pests and Effects of diseases transmitted by insects, reduction of seed production area, and time saving for picking anthers

Inactive Publication Date: 2013-10-16
TOBACCO RES INST OF HUBEI PROVINCE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] In the 1950s, my country had already carried out the utilization of tobacco heterosis and hybrid seed production, but in the process of production promotion and application, due to the poor quality of the bred varieties and the high production cost of hybrid seed production, it did not last long.
In recent years, with the development of the rural economy and the increase of farmers' income, the traditional tobacco male sterile hybrid seed production has the following disadvantages: first, the rural labor force is small and pollinators are scarce; second, it is greatly affected by the weather, There is no standard for seed yield; the third is low pollination efficiency and low seed yield (4-6kg); the fourth is high production cost

Method used

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  • Economical and efficient production method of tobacco male sterile first generation hybrid seed
  • Economical and efficient production method of tobacco male sterile first generation hybrid seed
  • Economical and efficient production method of tobacco male sterile first generation hybrid seed

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0039] Example 1. Effects of Different Ecological Conditions on Tobacco Hybrid Seed Yield and Quality

[0040] According to Table (1) the statistical results of seed yield and quality of tobacco hybrids in different seed production locations for 29 years from 1982 to 2010, combined with Table (2) analysis of the main ecological factor parameters of tobacco hybrid seed production areas over the years, it is considered that the seed yield of tobacco hybrids The quality is closely related to the altitude, sunshine hours, rainfall, average temperature, active accumulated temperature of 12°C-22°C and soil types in the seed production area, especially the sunshine hours during the flowering and pollination of tobacco plants and the growth and maturity of capsules from July to September The number, rainfall, and average temperature directly determine the yield and quality of seeds.

[0041] For example, from 1985 to 1986, the altitude of Jiulongtan Village, Gaoping Town, Jianshi Coun...

example 2

[0047] Example 2: The inventors conducted a comparative test in 2011 using an economical and efficient seed production method of the first generation of tobacco male sterile hybrid seeds (hereinafter referred to as the seed production method of the present invention) and a conventional seed production method according to the present invention. :

[0048] The test site is in Yangqueba Village, Wuyangba Office, Enshi City. The variety is Eyan No. 1, with an area of ​​0.5 mu. The geographical environment of this place and the climatic conditions are in line with the breeding method of the present invention through the analysis of Example 1. Seed production can be carried out after selecting the required environmental and climatic conditions. The specific test methods and results are as follows:

[0049] (1) adopt the concrete seed production step of the seed production method of the present invention:

[0050](1) Seedling cultivation: The method of floating seedling cultivation ...

example 3 012

[0086] Example 3 Comparison of production demonstration results between the seed production method of the present invention and conventional seed production methods in 2012: the demonstration site is in Yangqueba Village, Wuyangba Office, Enshi City, and the varieties are Eyan No. 1 and Eyan No. 3.

[0087] (1) The specific demonstration method is the same as the test method in Example 2.

[0088] (2) Demonstration results

[0089] 1. See Table (4) for output and quality comparison:

[0090] For Eyan 3, the average yield of the seed production method adopted by the invention is 26.08kg / mu, which is 16.62kg / mu higher than that of the control 9.46kg / mu, and the growth rate is 175.69%.

[0091] The average yield of Eyan No. 1 using the invented seed production method is 26.46kg / mu, which is 17.40kg / mu higher than the control 9.06kg / mu, and the growth rate is 192.05%.

[0092] The average yield of the two varieties of the invented seed production method is 26.27kg / mu, an increase ...

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Abstract

The invention provides an economical and efficient production method of a tobacco male sterile first generation hybrid seed. The method includes: at an altitude of less than 700 meters, under the climate conditions meeting the requirements of early vegetative growth and later reproductive growth of tobacco plants, conducting seedling culture on a maternal tobacco plant and a paternal tobacco plant separately, then employing isolation planting, performing topping on the maternal tobacco plant, then collecting paternal pollen and preparing pollination, during pollination, adding starch and blending them, adopting an artificial pollination pen or pollination gun to perform pollination, carrying out deblossoming on the maternal tobacco plant both before and after pollination, and when the capsule pericarp browning reaches more than 80%, conducting single fruit harvesting in batches and performing drying, then conducting threshing processing to obtain the male sterile first generation hybrid seed. The method provided in the invention has the advantages of reasonable design, low production investment, and high seed yield. On the basis of ensuring a variety purity (99%) and a seed germination rate (95%), maximization of a high-quality germplasm propagation coefficient is realized, the seed yield is greatly improved, and the seed production cost is decreased significantly.

Description

technical field [0001] The invention relates to a method for propagating crop seeds, in particular to an economical and efficient seed production method for tobacco male sterile hybrid first generation seeds. Background technique [0002] In the 1950s, my country had already carried out the utilization of tobacco heterosis and hybrid seed production. However, when it was popularized and applied in production, it did not last long due to the poor quality of the bred varieties and the high production cost of hybrid seed production. In the 1970s, after the introduction of the Burley tobacco male sterile variety MSB21 from the United States, the emasculation problem in hybrid seed production was solved, and the utilization of tobacco male sterile hybrids became active again (China Tobacco Cultivation. Shanghai Science and Technology Publishing Company; 1st edition, November 1, 2005). Up to now, a large number of new varieties of male sterile hybrids such as flue-cured tobacco, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02
Inventor 李宗平祖秉桥李进平张俊杰杨丽萍郭宇龙徐世平王文明彭灏陈茂胜
Owner TOBACCO RES INST OF HUBEI PROVINCE
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