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90results about How to "Antagonistic" patented technology

Bacillus velezensis HNt7 with broad-spectrum antibacterial activity as well as preparation method and application

ActiveCN108330089ANo preventionAntagonisticBiocideBacteriaBacteroidesDisease
The invention discloses bacillus velezensis HNt7 with broad-spectrum antibacterial activity as well as a preparation method and application. The antagonistic bacterium bacillus velezensis HNt7 has a number of CCTCC (China Center for Type Culture Collection) M 2018022. The preparation method comprises the following steps: A, selecting a bacillus velezensis HNt7 strain individual bacterial colony under a sterile condition and inoculating into a culture medium; adjusting the temperature and carrying out shake culture; B, filling a fermenting solution into each bottle and adding individual bacterial colony bacterium liquid according to the ratio; putting the bottle into a large-amplitude constant-temprature freezing shaking table and carrying out the shake culture; C, separating the antagonistic bacterium: immersing with alcohol with concentration and rinsing with sterile water; carrying out dark culture in a culture box and transferring a grown bacterial colony into a new culture medium and carrying out lineation separation and purification on the bacterial colony; D, after separating and purifying, obtaining the bacillus velezensis HNt7. The bacterium is used for preparing a medicinefor treating or preventing pear fruit rot disease. The strain has a remarkable disease-prevention effect and the prevention effect reaches 70.76 percent or more. After the bacillus velezensis HNt7 isapplied to pear trees, advanced defoliation of the pear trees is remarkably inhibited and the tree vigor is easy to enhance.
Owner:HUAZHONG AGRI UNIV

Environment-friendly fertilizer for strawberries and preparation method thereof

The invention discloses an environment-friendly fertilizer for strawberries and a preparation method thereof. The environment-friendly fertilizer is prepared from the following raw materials in parts by weight: 70-80 parts of organic fertilizer, 10-25 parts of inorganic fertilizer, 0.5-1 part of minor element, 0.1-0.3 part of fermentation composite bacteria, 1-2 parts of ferment bacteria, 3-7 parts of humic acid, 3-7 parts of amino acid and 0.2-0.7 part of antibiotics. The method comprises the following steps: uniformly mixing the fermentation composite bacteria with water to obtain a dilute bacterium solution; adding the dilute bacterium solution into the organic fertilizer, uniformly mixing, piling, and circularly overturning to obtain primary leavening; adding the ferment bacteria, fermenting until the materials are completely rotten, drying at low temperature, and pulverizing to obtain secondary leavening; and finally, adding the inorganic fertilizer, minor element, humic acid, amino acid and antibiotics, uniformly mixing, granulating, and sorting to obtain the environment-friendly fertilizer for strawberries. The environment-friendly fertilizer for strawberries is mainly prepared from agriculture and animal husbandry wastes, thereby solving the problem in crop nutrient supply and restricting the pathogenic microorganisms from reproduction.
Owner:WUHAN RUIZE SOURCE BIOLOGICAL ENVIRONMENTAL PROTECTION TECH

Bacillus subtilis

The invention relates to bacillus subtilis CH-001, which was collected in China Center for Type Culture Collection (CCTCC) on April 8th, 2011. The collection number is CCTCC NO: M 2011111. The bacillus subtilis has the characteristic of degrading keratin in a broad spectrum manner and has mild degradation and fermentation conditions, high enzyme production activity and high stability. The bacillus subtilis can generate high-activity keratinase, can degrade collagen, has antagonism on pathogenic bacteria such as staphylococcus aureus and the like, has the characteristics of probiotics, and is applicable to engineering bacteria development for fermentation production of animal protein feed additives and keratinase. The bacillus subtilis avoids the conflict between high-activity keratinase generated by most microorganisms and pathogenicity of the microorganisms on human or poultry and the broad spectrum characteristic on the keratin, and the antagonism on the related pathogenic bacteria is researched. The bacillus subtilis is a low-carbon, economic, high-efficiency, safe and green biological resource and has huge potential value for creation of economic and social benefits.
Owner:HUNAN AGRICULTURAL UNIV

Bacillus subtilis Y13 for effectively preventing and treating camellia anthracnose and use thereof

The invention discloses a Bacillus subtilis Y13 with the preservation number of CCTCC NO.M 208264, for effectively controlling oil tea anthrax, pertaining to the field of plant protection technology. At the same time, the invention discloses its separation method and applications, the Bacillus subtilis Y13 is obtained by flat plate facedown experiment screening, and can effectively inhibit the growth of oil tea anthrax bacteria, which has high proofing effect, good environment safety and favorable development and application prospects.
Owner:CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY

Oil-tea disease-resistant bacterial strain and application thereof

The invention discloses an oil-tea disease-resistant bacterial strain and an application thereof, in particular relates to an oil-tea anthracnose-resistant bacterial strain belonging to the technical field of plant protection. The bacterial strain is Bacillus brevis Z26, and the preservation number of the bacterial strain is China center for type culture collection (CCTCC) No: M20110113. The oil-tea disease-resistant bacterial strain is obtained by first screening by means of the a confrontation test and second screening by means of liquid fermentation liquid , can be effectively preventing and curing various plant diseases such as oil-tea anthracnose, oil-tea root rot, oil-tea half mad, citrus anthracnose, citrus root rot, aloe root rot and the like, particularly can be used for effectively restraining the growth of oil-tea anthracnose germ, is high in prevention efficiency, is good in environment security, and is very good in development and application foreground.
Owner:CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY

Cadmium-contaminated soil remedying agent

The invention relates to a cadmium-contaminated soil remedying agent, and belongs to the field of soil remediation. Attapulgite and isocyanate are mixed and modified by a silane coupling agent, so that a molecular bridge between an inorganic component interface and an organic component interface can be built to enhance the acting activity; meanwhile, Zn<2+> contained in salt can antagonize cadmiumions, so that absorption of the cadmium ions by plants and the toxic effect of the cadmium ions on crops are reduced, the mobility of the cadmium ions in soil is reduced, and the effects of passivating and remedying cadmium-contaminated soil are achieved; white mica and basalt are treated to form a three-dimensional crosslinked network so as to fix cadmium and reduce the bioavailability of the cadmium in the soil; protein can be destroyed by the cadmium ions, so that the viscosity is increased and a spatial structure becomes loose; the cadmium ions can be wrapped, so that the effect of reducing the mobility of the cadmium ions in the soil is achieved; and the problem that the current soil remedying agent commonly used for the cadmium-contaminated soil has poor remedying effect on the cadmium-contaminated soil is solved.
Owner:CHANGZHOU UNIV

Efficient chicken manure fermentation composite bacterium preparation

The invention belongs to the field of waste resource utilization, and particularly relates to an efficient chicken manure fermentation composite bacterium preparation. According to the invention, thepreparation is characterized in that the preparation is prepared from a yeast composition preparation with the total inoculum concentration being 5 percent and a lactobacillus composite preparation with the total inoculum concentration being 5 percent; the yeast composition preparation is prepared from Wickerhamomyces anomalus, candida tropicalis and candida utili according to the inoculum concentration ratio of 1:(2 to 3):(2 to 3), and is used for the aerobic fermentation of the chicken manure; the lactobacillus composite preparation is prepared from lactobacillus acidophilus, lactobacillus plantarum and lactobacillus farciminis according to the inoculum concentration ratio of 1:1:1, and is used for the anaerobic fermentation of the chicken manure. The chicken manure fermentation composite bacterium preparation provided by the invention comprises an aerobic fermentation preparation and an anaerobic fermentation preparation, so that the conventional whole-process anaerobic fermentationidea is broken; the real protein content in the chicken manure is effectively improved; the feeding value of the fermentation chicken manure is increased. The preparation can be directly used in practical production and has the important guidance significance on the subsequent further study.
Owner:CHENGDU INST OF BIOLOGY CHINESE ACAD OF S

Special disinsection and sterilization polyelement microorganism fertilizer for preventing cabbage downy mildew

The invention discloses a special disinsection and sterilization polyelement microorganism fertilizer for preventing cabbage downy mildew. The special disinsection and sterilization polyelement microorganism fertilizer comprises components of raw materials in parts by weight as follows: 3-5 parts of bacillus subtilis, 1-4 parts of trichoderma reesei, 4-8 parts of a fertilizer leavening agent, 1000-1100 parts of an organic fertilizer, 350-500 parts of an inorganic fertilizer, 65-85 parts of a trace element, 5-7 parts of a plant growth regulator, 25-35 parts of an adhesion agent, Chinese herbal medicinal ingredients and the like. The special disinsection and sterilization polyelement microorganism fertilizer for preventing the cabbage downy mildew can reduce the morbidity of the cabbage downy mildew greatly, and reduce bacterial and fungal diseases of leaves of a plant obviously, the plant can grow more vigorously, the yield of cabbages isincreased greatly, the quality is improved, and probiotics in the soil are also increased.
Owner:SUZHOU RENCHENG BIO SCI & TECH

5-methyl-1,2,3-thiadiazole-containing 1,3,4-thiadiazole derivatives and their preparation method and use

The invention provides 5-methyl-1,2,3-thiadiazole-containing 1,3,4-thiadiazole derivatives and their preparation method and use. The 5-methyl-1,2,3-thiadiazole-containing 1,3,4-thiadiazole derivatives are 1,2,3-thiadiazole-containing heterocyclic compounds. Intermediates of the 5-methyl-1,2,3-thiadiazole-containing 1,3,4-thiadiazole derivatives and the 5-methyl-1,2,3-thiadiazole-containing 1,3,4-thiadiazole derivatives respectively have a general structural formula III and a general structural formula IV. The invention discloses the general structural formula and the preparation method of the 5-methyl-1,2,3-thiadiazole-containing 1,3,4-thiadiazole derivatives, and also discloses a use of the 5-methyl-1,2,3-thiadiazole-containing 1,3,4-thiadiazole derivatives as bactericides, plant virus resistance agents and plant activators. After being mixing with agriculture acceptable assistants or synergists, the 5-methyl-1,2,3-thiadiazole-containing 1,3,4-thiadiazole derivatives can be used for preparation of bactericides, plant virus resistance agents and plant activators. The invention also discloses a use of the 5-methyl-1,2,3-thiadiazole-containing 1,3,4-thiadiazole derivatives combined with commercial insecticides, bactericides, plant virus resistance agents and plant activators in prevention and treatment on agricultural, forest and gardening diseases, insect pests and viral diseases, and also discloses a preparation method thereof.
Owner:NANKAI UNIV

Streptomyces tubercidicus strain and application thereof

The invention relates to a streptomyces tubercidicus strain and an application thereof. The streptomyces tubercidicus strain 7-2 is preserved in China General Microbiological Culture Collection Center, has the preservation number of CGMCC No.14965 and is preserved on November 28, 2017. The streptomyces tubercidicus strain 7-2 has antagonistic effects on phomopsis asparagi, magnaporthe oryzae, mushroom monilinia fructicola, colletotrichum truncatum and other various plant pathogens. Fermentation broth has good control effect on asparagus stem blight, rice blast, rice false smut, colletotrichumdestructivum and mushroom brown rot, and significantly improves the control effect of bactericides when being mixed with the bactericides.
Owner:INST OF PLANT PROTECTION FAAS
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