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Streptomyces tubercidicus strain and application thereof

A technology for streptomyces and nodules, applied in the direction of application, fungicides, bacteria, etc., can solve problems such as excessive use of fungicides, achieve good control effects and improve control effects

Active Publication Date: 2019-05-17
INST OF PLANT PROTECTION FAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The primary purpose of the present invention is to provide a Streptomyces tubercidicus bacterial strain that has an antagonistic effect on various crop pathogens against the problem of excessive use of fungicides in the process of crop disease control

Method used

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  • Streptomyces tubercidicus strain and application thereof
  • Streptomyces tubercidicus strain and application thereof
  • Streptomyces tubercidicus strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1: Isolation and identification of Streptomyces tubercidicus strain 7-2

[0033] 1. Collection of soil samples

[0034] Three samples of soil around the rhizosphere of Taxus chinensis were collected from Jinggangshan, Jiangxi. The soil on the surface was removed, and the soil samples at a depth of 5-20cm were collected, marked and brought back to the laboratory for natural air drying.

[0035] 2. Isolation of actinomycetes

[0036] Separation was performed using the plate dilution method. Grind the air-dried soil sample with a mortar, weigh 1 g of the sample and suspend it in 9 mL of sterile water, shake it at 40 °C and 180 rpm for 30 min, then let it stand for 5 min, dilute it by 10 times successively, and prepare 10 -2 、10 -3 、10 -4 0.1mL of suspensions with different concentrations were drawn and added to the improved HVA medium (potassium dichromate with a final concentration of 100-200ppm added) on the plate, evenly coated and placed upside down at 28°C...

Embodiment 2

[0050] Embodiment 2: Antagonistic determination of Streptomyces tubercidicus (Streptomyces tubercidicus) strain 7-2 to pathogenic bacteria

[0051] The antagonism of Streptomyces tubercidicus strain 7-2 against asparagus stem blight, rice blast fungus, soybean anthracnose fungus and mushroom brown rot fungus was determined by plate confrontation culture method. First, inoculate the strain 7-2 by streaking on both sides of the edge of the PDA medium, insert the bacterium cake of the pathogenic bacteria with a diameter of 5mm in the center of the plate after 3 days, and cultivate it at 28°C for 5 days to measure the antibacterial effect of the strain 7-2 on the pathogenic bacteria. Band width, taking the testing pathogenic bacteria that do not connect antagonistic bacteria as contrast, the result shows that bacterial strain 7-2 all has very good antagonistic effect to asparagus stem blight, rice blast fungus, soybean anthracnose fungus, mushroom brown rot fungus ( figure 2 ). ...

Embodiment 3

[0052] Embodiment three: kill Streptomyces tubercidicus (Streptomyces tubercidicus) bacterial strain 7-2 fermentation broth to the inhibitory action of pathogenic bacteria

[0053] Put the spores of the strain 7-2 activated by Gaoshi No. 1 medium into the ISP2 liquid medium, the liquid volume is 80mL / 250mL Erlenmeyer flask, the inoculum volume is 2-5%, the temperature is 25-28°C, and the rotation speed is 180-200rpm , fermented and cultivated for 5-7 days to obtain a fermented liquid, and then filtered through a 22 μm microporous membrane to obtain a sterile fermented filtrate, which is set aside.

[0054] Take 1mL of the sterile fermentation filtrate of strain 7-2 and add it to 9mL PDA medium, mix it and pour it into a petri dish, put the test pathogenic bacteria cake with a diameter of 5mm into the center of the PDA medium plate, and culture it at 28°C for 7 days Measure the colony diameter. Taking no aseptic fermentation filtrate as a contrast, calculate the inhibitory rat...

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Abstract

The invention relates to a streptomyces tubercidicus strain and an application thereof. The streptomyces tubercidicus strain 7-2 is preserved in China General Microbiological Culture Collection Center, has the preservation number of CGMCC No.14965 and is preserved on November 28, 2017. The streptomyces tubercidicus strain 7-2 has antagonistic effects on phomopsis asparagi, magnaporthe oryzae, mushroom monilinia fructicola, colletotrichum truncatum and other various plant pathogens. Fermentation broth has good control effect on asparagus stem blight, rice blast, rice false smut, colletotrichumdestructivum and mushroom brown rot, and significantly improves the control effect of bactericides when being mixed with the bactericides.

Description

technical field [0001] The invention relates to the technical field of agricultural crop disease prevention and control, in particular to a strain of Streptomyces nodosum and its application. Background technique [0002] Biological control of plant diseases refers to the technology and method of using beneficial microorganisms or microbial metabolites to effectively prevent and control plant diseases. Chen Yanxi, a Chinese plant pathologist, pointed out: "Biological control of plant diseases is to adjust the microbial environment of host plants in the agricultural ecosystem to make it beneficial to the host and not to the pathogen or to make it beneficial to the interaction between the host and the pathogen." The host is not conducive to the influence of pathogens, so as to achieve the purpose of disease prevention and control. The types of microorganisms used for plant disease control mainly include bacteria, fungi, and actinomycetes. Actinomycetes are an important class...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P1/00A01P3/00C12R1/465
Inventor 石妞妞杜宜新阮宏椿陈福如杨秀娟甘林代玉立
Owner INST OF PLANT PROTECTION FAAS
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