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Bacillus amyloliquefaciens with phosphate solubilizing, disease preventing and growth promoting functions and application thereof

A technology for dissolving starch spores and dissolving phosphorus, which is applied in applications, microorganism-based methods, and chemicals for biological control, etc., to achieve the effect of broad antibacterial spectrum and broad-spectrum antibacterial effect.

Active Publication Date: 2016-03-23
FOSHAN YANHUI BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report of Bacillus amyloliquefaciens that has both phosphorus-solubilizing effect and control of bacterial wilt, tobacco black shank, watermelon wilt, rice blast, rice sheath blight, strawberry powdery mildew, cucumber wilt and other diseases

Method used

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  • Bacillus amyloliquefaciens with phosphate solubilizing, disease preventing and growth promoting functions and application thereof
  • Bacillus amyloliquefaciens with phosphate solubilizing, disease preventing and growth promoting functions and application thereof
  • Bacillus amyloliquefaciens with phosphate solubilizing, disease preventing and growth promoting functions and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Example 1: Isolation of Bacillus with Phosphorus Solubilizing Effect

[0016] 167 strains of Bacillus were isolated from Hunan, Changping, Hebei, Liaoning and other places, 101 of which had phosphorus-dissolving activity.

[0017] 18 strains with high phosphorus-solubilizing intensity were screened. By measuring the aqueous solution phosphorus in 18 bacillus strain shake flask fermented liquid, determined each bacterial strain to the decomposition ability of inorganic phosphorus, organic phosphorus, as table 1, as can be seen from the result, decomposing inorganic phosphorus and decomposing organic phosphorus are not positively correlated, Based on the comprehensive understanding of organic phosphorus and inorganic phosphorus, we determined that five of them, JCD-H-12, JCD-H-16, JCD-Q-3, JCD-L-4, and JCD-L-16, had strong phosphorus-dissolving ability. The strain of action. Use the following two kinds of organic phosphorus and inorganic phosphorus bacterial culture med...

Embodiment 2

[0023] Embodiment 2 has the screening of the antibacterial test of the bacillus that has stronger phosphorus solubilizing effect

[0024] Put JCD-H-12, JCD-H-16, JCD-Q-3, JCD-L-4, JCD-L-16 in Pythium spp.,

[0025] Rice blast (Pyriculavia oryzaecav.), pepper early blight (Alternaria solanipepper), tomato bacterial wilt (Psevdomonassolanacearum), Rhizoctonia solani (Rhizoctoniasolani), powdery mildew (Erisyphespp.), tobacco black shank (Phytophthoraparasiticavar.nicotianae), Strawberry Botrytis (Botrytiscinera Pers.exFr.) and other pathogenic microorganisms were marked with a "cross" on the plate, and after 72 hours of constant temperature cultivation at 28°C, observe whether there is a zone of inhibition. The results are shown in Table 2 below, "+" means that there is a zone of inhibition, " -" means no obvious inhibition zone. It can be seen from Table 2 that JCD-H-12 has a wider antibacterial ability, and this bacterium will be used as the research purpose bacterium below. ...

Embodiment 3

[0028] Example 3 Identification of Bacillus amyloliquefaciens JCD-H-12 and Test of Broad Spectrum Antibiotic Properties

[0029] Through physiological and biochemical tests, Gram-positive bacteria, rod-shaped, endophytic spores, no flagella; milky white colonies formed on beef peptone medium, smooth surface, with ragged edges. Glucose fermentation test positive, starch hydrolysis test positive, gelatin liquefaction test positive, V.P reaction positive, contact enzyme reaction positive, methyl red reaction positive, nitrate reduction test positive, citrate utilization positive, aerobic growth, optimum pH The temperature is 6-8.5, the growth temperature is 28-38°C, and it is identified as Bacillus amyloliquefaciens by 16srDNA.

[0030] Take a ring of activated bacteria liquid from JCD-H-12 and cross-hatch it on a PDA plate, inoculate pathogenic bacteria at a distance of 3cm from the inoculation line, incubate at 28°C for 7 days, and measure the width of the inhibition zone. The...

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Abstract

The invention relates to bacillus amyloliquefaciens which has stronger phosphate solubilizing and plant growth promoting functions and has a better preventing and treating effect on various plant diseases. According to the bacillus amyloliquefaciens JCD-H-12 with the phosphate solubilizing, disease preventing and growth promoting functions, disclosed by the invention, the preservation number is CGMCC NO.11856, the preservation date is December 10, 2015, and multiple effects of solubilizing phosphate, preventing diseases, promoting the growth, increasing the yield and the quality, having a broad bacteriostatic spectrum and the like are obtained; a bacterial strain and inoculant of the bacterial strain are capable of effectively preventing and treating powdery mildew, tobacco black shank, botrytis blight, rice blast, cucumber fusarium wilt, tomato early blight, sweet pepper anthracnose, watermelon sclerotinia sclerotiorum, wheat scab, watermelon fusarium wilt, phytophthora capsici and the like; a broad-spectrum antibacterial effect is obtained, the effect is stable and durable, and the bacillus amyloliquefaciens can be developed into a bacterial fertilizer and microbial pesticide preparation which integrates phosphate solubilizing with disease preventing and treating.

Description

Technical field [0001] The present invention belongs to the field of microbial pesticides and microbial fertilizers. It is specifically involved in a alcoholic spores JCD-H-12, which has the effect of anti-phosphorus and prevention. Background technique [0002] The average content of phosphorus in the crust is about 0.122%. During the formation of soil, phosphorus has a certain degree of migration. With the process of soil, the soil phosphorus content is also reduced.Most of my country's soil phosphorus content is between 0.043%-0.046%, so in the soil, phosphorus content is relatively lacking.Because phosphorus is one of the 16 nutrient elements necessary for plant growth and development, the crops mainly absorb phosphorus in chemical forms such as phosphoric acid roots, and the phosphorus in the soil is mainly existed in a fixed mineral state, accounting for about 70 of the total phosphorus content.%, So the content of water -soluble inorganic phosphoric acid ions in the soil p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00A01G13/00C05G3/00C12R1/07
CPCA01G13/00A01N63/00C05G3/00C12N1/20C12N1/205C12R2001/07
Inventor 胡艳晖其他发明人请求不公开姓名
Owner FOSHAN YANHUI BIOTECH CO LTD
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