Bacillus licheniformis 202 for highly effective preventing and curing banana wilt and application thereof
A technology of Bacillus licheniformis and banana wilt bacteria, applied in the direction of application, bacteria, fungicides, etc., can solve the problems of blank and weak technology, and achieve the effects of easy preservation, fast reproduction speed and strong inhibitory effect
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Embodiment 1
[0020] Example 1 Plate Antagonism Experiment of Bacillus licheniformis 202 on Banana Fusarium wilt No. 4 Race FOC4
[0021] Insert a FOC4 mycelium block with a diameter of 5mm in the center of the potato sucrose medium PDA plate, and then put a sterilized filter paper sheet with a diameter of 5mm on the triangle of the equidistant FOC4 mycelium block, and insert Bacillus licheniformis on each filter paper sheet 10 μL of 202 culture solution, take the plate without Bacillus licheniformis 202 culture solution as a control, culture in an incubator at 25-28°C for 5-7 days, and measure the diameter of the inhibition zone. It can be seen that Bacillus licheniformis 202 has a good effect on banana wilt Strong antibacterial effect.
Embodiment 2
[0022] Example 2 Bacillus licheniformis 202 prevents the main root and vascular bundle of banana plants from browning or rotting experiments, and the control effect of greenhouse potted plants.
[0023] Select Brazilian banana seedlings with a plant height of about 15 cm, and carry out 3 different treatments: (1) clean water healthy plant control: the roots of banana seedlings are soaked in sterile water and then directly transplanted in a nutrient bowl to grow; (2) the roots of banana seedlings are soaked in sterile water. After 30 minutes in the culture medium of Bacillus licheniformis 202, transplant it into the nutrient bowl, then pour 20mL of FOC4 spore suspension into the soil of the nutrient bowl; (3) Incidence control: Roots of banana seedlings were inoculated with 20mL of FOC4 spores Suspension, not inoculated with Bacillus licheniformis 202 culture solution. For the above treatments, there were 20 plants in each treatment group. Afterwards, they were cultured at 26°...
Embodiment 3
[0024] Example 3 Antagonism of Bacillus licheniformis 202 to various pathogenic bacteria
[0025] The antagonism of Bacillus licheniformis 202 to 7 pathogenic bacteria including tomato fusarium wilt, Curvularia maize leaf spot, Glue sporotrichum anthracnose, cotton fusarium wilt, cotton Verticillium dahliae, wheat scab and watermelon wilt was tested by plate confrontation method effect, insert a target mycelium block with a diameter of 5mm in the center of the PDA plate, and then put a sterilized filter paper sheet with a diameter of 5mm on the triangle of the equidistant FOC4 mycelium block, and insert Bacillus licheniformis 202 into each filter paper sheet for cultivation 10 μL of solution, and the plate without Bacillus licheniformis 202 culture solution was used as a control, cultured in a 28°C incubator for 5-7 days, and the diameter of the inhibition zone was measured. Bacillus licheniformis 202 has a wide antibacterial spectrum and has antagonistic effects on various pa...
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