The invention provides a driver of a digital
confocal microscope optical section collecting device. The driver comprises a single-
chip system, a sub-division driver, a stepping motor and a displacement driving device, and the driver arranged on the
microscope and controlled by a computer drives a stage to acquire the sequenced optical section images of cells on the stage by an
image detector. The method for the driver to acquire the optical section images is to carry out the submicron
equidistant stepping control on the stage of the
microscope by setting the section distance, collected section number and other parameters. By using the definition-based evaluation for the function value and the change rate thereof as the evaluation standards, the method of the invention is capable of automatically focusing
on cells and locating the upper and lower edges, and acquiring the sequenced optical section images of intact cells.