1064 results about "Bacillus natto" patented technology

The invention relates to micro-ecological Chinese medicine preparation for livestock and poultry by adoption of multi-strain fermentation production and a fermentation method. The compositions of traditional Chinese medicines in a culture medium of the fermentation preparation include: radix astragali, radix codonopsitis, Atractylodes macrocephala, Poria cocos, liquorice, medicated leaven, hawkthorn, angelica, Chinese rhubarb, Scutellaria baicalensis, radix isatidis, cordate houttuynia, sicklesenna seeds, Schisandra chinensis, Gynostemma pentaphylla, phellodendron, dried orange peel, radix bupleuri, curcuma, honeysuckle, Chinese gall, purslane and Quisqualis indica. The fermentation method is as follows: a multi-strain solid state fermentation strain is adopted for fermentation; bacillus subtilis, bacillus natto, bacillus licheniformis, beer yeast, Candida wtilis, Aspergillus niger, lactobacillus plantarum, Lactobacillus acidophilus and bifidobacteria are purified and subjected to anaerobic fermentation under the conditions of a rotating speed of 180 revolutions per minute at a temperature is 32 DEG C for 48 to 72 hours till the pH value reaches 4.0. The micro-ecological Chinese medicine preparation has the advantages that the micro-ecological Chinese medicine preparation uses probiotics to ferment different Chinese medicine compositions, and is micro-ecological Chinese medicine preparation which completely replaces antibiotics and has the advantages of no residual medicine, disease prevention, growth promotion and low cost.

The invention discloses a compound microbial culture starter and application thereof, which belong to an application of a beneficial microbial preparation at low cost. The active microorganism of the compound microbial culture starter comprises saccharmyces cerevisiae, bacillus subtilis, bacillus natto and lactococcus lactis. The compound microbial culture starter can be used for livestock and poultry raising beds, feeds addictive and other aspects and is characterized by reducing the cost input of users on the culture starter and having stable effect.

The invention provides compound probiotics fermented Chinese herbal medicine active health care liquid and a preparation method thereof. The health care liquid is prepared by the following raw materials: folium ginkgo, gingko pollen, fructus lycii, tea leaves, bacillus natto, saccharomyces cerevisiae, lactobacilli, acetobacter xylinum, bifidobactirium, white granulated sugar, brown sugar, defatted soy flour, honey, oligosaccharide, sodium chloride, deionized water and the like. The compound probiotics fermented Chinese herbal medicine active health care liquid integrates disease treatment and health care functions of several single products, contains Chinese herbal medicine active ingredients with the functions of preventing and treating hyperlipidemia, hyperglycemia and hypertension, preventing tumors and cancers, enhancing memory, protecting livers, improving immunity, preventing and treating coronary heart diseases, and also contains probiotics groups and metabolic products of the probiotics groups which can regulate balance in the stomach and intestines, resist aging, prolong the life, facilitate digestion, expel toxin for beauty, and prevent and treat thrombi, constipation and diarrhea. The health care liquid integrates health care and treatment, is very wide in market prospect, and conforms to the existing medical viewpoint of people that the prevention, health care, treatment and recovery are combined.

The invention discloses a compound microorganism additive for milk cow feed. The microorganism additive consists of fermentation product mixture of natto bacillus subtilis, enterococcus faecium and saccharomyces cerevisiae, wherein the total number of bacteria is more than 10<9>cfu / g. Long-term repeating animal experiments show that three microorganisms in the compound microorganism additive can develop synergy, thereby significantly improving the milk yield of the milk cow, fat percentage in the milk and milk protein yield, significantly reducing the somatic number of the milk cow in lactation period and the disease probability of mastitis and subclinical mastitis, and significantly enhancing the autoimmunity of the milk cow organism. The compound microorganism additive has the advantages of low cost, significant effect and good application and market prospects.

The invention relates to integrated enzyme powder having effects of reducing fat and losing weight. The integrated enzyme powder is characterized by being prepared from powders which are, by weight parts, 62.8-71.8 parts of integrated enzyme raw powder, 8-15 parts of konjac flour, 10 parts of pumpkin powder, 1 part of papaya powder, 3 parts of pineapple powder, 1 part of mango powder, 1 part of sweet orange powder, 1 part of snow pear powder, 3-5 parts of dietary fiber powder and 0.2 part of compound lactobacillus powder. 43 fruits, 20 vegetables, 4 cereal nuts and 11 medicinal and edible herbaceous plants and natto are selected in total to serve as fermentation raw materials; through yeast and lactobacillus fementation, generated enzymes are total nutrients needed by humans, and the enzyme powder contains activity probiotics which are resistant to acid and heat; and through testing, the probiotics in artificial gastric juice still have ultrahigh vigor; at normal temperature (37 DEG C), the probiotics still maintain activity, can regulate intestinal flora of the human body, promote digestion and avoid constipation. The mixed integrated enzyme powder not only can achieve the goal of losing weight, but also can meet the basic nutrition requirements of the human body, and it is a healthy and effective method of reducing fat and losing weight to use the enzyme powder.

The invention relates to a feeding addition agent for microorganism viable bacteria and a preparing method thereof. The addition agent consists of 1.5-2.5 parts of bacillus coagulans viable bacteria powder, 0.5-2.0 parts of lactobacillus acidophilus viable bacteria powder, 1.0-2.5 parts of lactobacillus plantarum viable bacteria powder, 0.5-2.5 parts of bacillus natto viable bacteria powder, 0.5-1.5 parts of candida utili viable bacteria powder and 2-4.5 parts of aspergillus niger viable bacteria powder. Each gram of the content of viable bacteria is as follows: bacillus coagulans is not less than 1.0*10cfu / g; lactobacillus acidophilus is not less than 1.0*10cfu / g; lactobacillus plantarum is not less than 1.0-10cfu / g; bacillus natto is not less than 1.0*10cfu / g; aspergillus niger is not less than 1.0*10cfu / g; and candida utili is not less than 1.0*10cfu / g. The invention has the advantages that the composition is reasonable, the preparation method is scientific, the usage scale is wide, the operation is simple and easy to master, and the cost is low. In addition, the invention can be used as the feeding stuff agent for various poultries, thus increasing the use ratio of the feeding stuff addition agent and strengthening the anti-disease ability of the poultries.

The present invention relates to the field of human nutrition, and in particular to pharmaceutical compositions and nutritional supplements comprising an effective daily dose of menaquinone 7. In particular, the present invention provides pharmaceutical compositions, nutritional supplements and food products that comprise from about 10 to about 200 micrograms of menaquinone 7. In some embodiments, the menaquinone 7 is provided in a natto extract.

The invention relates to a fermented liquid feed for pig and poultry as well as a preparation method and an application thereof, belonging to the field of biotechnology, in particular to a liquid feed produced by the fermentation of a plurality of beneficial microbes and Chinese medicinal herbs as well as a preparation method and an application thereof. The preparation method has simple process and comprises the following steps: after primary slant culture and secondary solution culture, lactobacillus, fermentation lactobacillus, bacillus subtilis, natto bacillus and saccharomyces cerevisiae are put into a solid fermenting tank with a fermentation medium containing astragalus root, Na2HPO4, nitrogen source and carbon according to weight proportion. The fermented liquid feed adopting the method has high quality, thicker mellow flavor and short fermenting time, contains protein, polypeptide, small peptide, amino acid, astragalus polyose and a plurality of beneficial microbes and is applicable to preparing various perfect compound feeds for pig and poultry.

The invention relates to a method for fermentation production of a poultry bacterial agent by multi-bacteria miscible liquid, wherein aerobic bacteria, namely Bacillus subtilis, Bacillus licheniformis, bacillus natto, beer yeast, Aspergillus niger and Aspergillus oryzae are cultured in a shaking table according to different culture mediums, so as to culture a mother seed solution; simultaneously Lactobacillus acidophilus, bifidobacteria and enterococcus faecalis are subjected to anaerobic culture by utilization of Kille flasks, and a mother bacterial solution of photosynthetic bacteria is cultured by a Kille flask under the condition of illumination; the prior stock solution is inoculated into a seed tank for anaerobic culture and fermentation according to 4 percent of the inoculum concentration, and the fermentation time is between 48 and 60 hours; and the cultured seed liquid is inoculated into a productive tank for anaerobic culture and fermentation according to 10 percent of the inoculum concentration, the fermentation time is between 60 and 72 hours, and the fermentation end point is reached when the pH value is reduced to 4.0. The method has the advantages that the microscopic examination viable count of the bacterial agent is more than 5 billion per milliliter, so that the bacterial agent is safe and nontoxic, thereby not only improving the disease resistance of poultry but also promoting the quick growth of the poultry, reducing the feed-meat ratio and improving the quality of meat, eggs and milk.

The invention provides a drink and a sauce having rich nutrients and multiple health-care functions, which are prepared by leaching fermented soybeans with added vinegar (wine, fruit juice, water or tear), deep processing and adding raw sugar and other auxiliaries containing rich nutrients and bioactivators. Soybeans undergo germination, fermentation and other extremely complex biochemical processes to form hundreds of nutrients and activators with the nutrients of the soybeans. A large number of Chinese and foreign documents prove that the substances have health care functions such as human body free radical clearing, arteries hardening prevention, immunity improvement, cancer resistance, blood fat and cholesterol reduction, blood pressure reduction, blood sugar reduction, bowel loosening for relieving constipation, intestinal microecology improvement, beneficial bacteria propagation and bacterium resistance and suppression. The invention aims to solve a technical problem that the nutrients were not fully used by Chinese people in the past 2,000 due to high salinity (such as that of lobster sauce, soybean paste, preserved bean curd and soy sauce) and disagreeable flavor (such as that of natto and tempe). The invention provides a novel drink and a novel sauce which contain nutrients and bioactivators of fermenting soybeans, are edible and contribute to absorption. Till now, no records of the uses of the soybean food have been found in Chinese and foreign documents. The drink and the sauce fill the gap of health care products in China, are expected to contribute to the promotion of the health care cause in China.

The invention relates to a microbial agent for purifying river wastewater and a preparation method of the microbial agent. The microbial agent comprises the following components in parts by weight: 20-40 parts of bacillus natto, 10-30 parts of nitrobacteria, 5-15 parts of white-rot fungi, 5-15 parts of pseudomonas and 10-40 parts of photosynthetic bacteria. The microbial agent is well-adapted and has the capabilities of fast forming dominant bacterial communities and replenishing indigenous rhizobia to the water body; and the microbial agent can be used for reducing the COD (Chemical Oxygen Demand), NH3-H, total nitrogen, TP (Total Phosphorus) and the like, removing odor and color and producing high-activity bio-enzyme to suppress the growth of harmful bacteria and acceleratively decompose residual baits as well as animal and plant residues, and a remarkable water quality purifying effect is achieved.

The invention discloses bacillus natto which is classified and named bacillus natto and has been preserved in the China Center for Type Culture Collection with the preservation number of CCTCC M 2014405. The strain is extracted from a natto product purchased in the market and is obtained through ultraviolet mutagenesis and screening. By the use of the strain, soybean protein can efficiently be utilized to synthesize vitamin K2. The invention also discloses a method for producing vitamin K2 grease by the utilization of the above bacillus natto. According to the invention, a strain fermentation technology is optimized through a novel oxygen supply strategy, and fermentation cylinder scale-up is carried out. The final output of vitamin K2 reaches 60.54 mg / L. Conversion rate relative to soybean protein is 2.018 mg / g. Production costs are low. By the above method, efficient production of vitamin K2 grease can be realized; the whole process is simple; operation is convenient; production cycle is short; content of vitamin K2 is high; and fermentation cost is reduced. The method is suitable for industrial production.

The present invention discloses a kind of natto powder, and the natto powder is prepared through the following steps: soaking soybean in water for 10-24 hr; steaming at temperature over 100 deg.c for 20-60 min and cooling the cooked soybean to 20-40 deg.c; spraying natto bacteria liquid and fermentation at 35-45 deg.c and 50-90 RH% for 18-30 hr; cold storing the fermented natto soybean with rich transparent baves at 0-10 deg.c for over 24 hr; and freeze drying, crushing and 40-80 mesh sieving. The application of the natto powder is also disclosed, and the natto powder may be applied in serial health foods with the health functions of dissolving thrombus, balancing blood pressure, preventing cardiac and cerebral vascular diseases, etc.

The invention discloses a cosmetic and a preparation method thereof. The cosmetic is prepared from five components of A, B, C, D and E. The cosmetic is composed of thirty raw materials including deionized water, hydroxyethyl cellulose, allantoin, carbopol U20, propanediol, 1,3-butanediol, sodium hyaluronate, an aloe vera extract, a natto extract, asiaticoside, vitamin C, bisabolol, an amino acid humectant, D-panthenol, VC ethyl ether, nicotinamide, glabridin, tranexamic acid, ceramide, algal polysaccharide, glucan, dipotassium glycyrrhizinate, an epidermal growth factor (EGF), triethanolamine, isothiazolinone, essence, squalane, jojoba oil, vitamin E oil and crystal sparkling pearl powder. The cosmetic has the functions of making skins fine and smooth, brightening the skins, repairing sensitive skins, controlling oil, toning skins and modifying skin blemishes, has no side effect or no dependence, and does not threaten health of a human body.

The invention discloses a composite bacillus preparation applied to improving the aquatic environment of mariculture, and a making method thereof. The preparation is made by mixing Bacillus subtilis, Bacillus licheniformis, Bacillus cereus and Bafillus natto according to certain ratio, and under room temperature, the active microorganism is in resting state and the preparation is stable and easy to be preserved. After the composite bacillus preparation is put into mariculture water, the dormant bacteria can be back to normal quickly, degrade residual bait, dejection, fertilizer, biological residual bodies and other organic contaminant fast, reduce the content of ammonia nitrogen, nitrite and COD in the culture water and reproduce to be predominant bacteria in the water quickly so as to further restrain the putrefying bacteria from reproduction, improve the environment of the culture water and purify the water. The preparation of the invention is safe, contains no pollution nuisance and residual, can obviously improve the environment of the culture water, and meanwhile, the immunity of aquatic livestock can be improved.

The invention provides a compound microorganism agent for treating a black and odorous water body. The compound microorganism agent comprises compound bacteria and a compound enzyme preparation, wherein the compound enzyme preparation comprises candida tropicalis, candida utilis, bacillus natto, bacillus subtilis, bacillus licheniformis, nitrobacter, achromobacter denitrificans, nitrosomonus, acinetobacter, lactobacillus, aspergillus niger, aspergillus oryzae and photosynthetic bacteria; the compound enzyme preparation comprises protease, amylase, lipase, sucrase, and cellulase.

The invention discloses an antibiotic complex aquatic feed additive which is prepared from the following components in percentage by weight: 1-2 percent of probiotics, 8-9 percent of prebiotics and 20-30 percent of carriers. The probiotics comprise natto bacillus, bacillus coagulans and clostridium freeze-dried powder, the prebiotics comprise xylo-oligosaccharide, ganoderma meat amylase and oligochitosan, and the carriers comprise montmorillonite and corn starch. The antibiotic complex aquatic feed additive not only has the advantages of stable thalli and long survival time, but also has the effectiveness of united multiple roles. the invention also provides a mixed feed containing the antibiotic complex aquatic feed additive, which can obviously improve the digestion function of animals, increase the growth performance of animals of fishes, shrimps, soft-shelled turtles, and the like, decrease the feed factor, enhance the immunity of the animals and obviously improve the meat quality of animals.

A method for preparing solid natto bacillus microecological agent by solid fermenting is disclosed, which belongs to the biological engineering technique field. The strain is separated from natto as natto bacillus which is named as Bacillus natto S703 and the preservation number of the strain is CCTCC M207147. The sieving method comprises the following steps: utilizing the character that the natto bacillus produces natto kinase to do dilute flat separation for natto leach, seeding the obtained single colony in the primary sieving culture medium of the flat which contains fiber, selecting single colony to purify and obtain the fitful natto bacillus according to the size of transparent coil diameter and colony diameter. The strain is inoculated in the solid ferment culture medium which comprises bran, soya bean waste, corn starch, dipotassium hydrogen phosphate and magnesium sulfate, which can produce the natto bacillus microecological agent by solid fermenting, drying and grinding. The prepared natto bacillus microecological agent can be chicken feed, which is provided with obvious modification for daily chicken weighing, feed coefficient and survival rate.

The invention relates to a bacillus subtilis subso natto for producing natto kinase and application thereof. The bacillus subtilis subso natto (bacillus subtilis subso natto LNUB236), CCTCC No.: M 208156. The bacillus subtilis subso natto is subject to activation of culture, seed liquid and fermentation liquor are prepared, and the natto kinase is extracted. The invention relates to application of the natto kinase to preparing a health product for preventing and treating cerebral thrombosis. Constituents of the health product are proportioned by weight: lyophilized natto powder : natto kinase : natto kinase : evening primrose oil=1:2.0-3.0:1.0-1.5. The bacillus subtilis subso natto LNUB236 has high natto kinase yield, the highest enzyme activity is 431.455IU / ml in the event of production in a 30l fermenter, and the prepared natto kinase-containing health product has the advantages of high enzyme activity, obvious effect and no side effect.

The solid fermentation process for producing natto bacillus feed additive with bean dregs includes the following steps: 1. shake flask culture of natto bacillus in KMB culture medium to obtain the first stage spawn; 2. dissolving bean dreg powder, corn starch, corn slurry and NaCl in water, regulating pH value, sterilizing and inoculating the first stage spawn, and shake flask culture to obtain the second stage spawn; 3. mixing water soaked bean dregs, NaCl, glucose as the carbon source, gelatin as the nitrogen source and (NH4)2SO4 or urea, and sterilizing to obtain solid culture medium, inoculating the second stage spawn, fermenting while maintaining the temperature and humidity to obtain fermented bean dregs; and 4. adding sterilized corn powder into the fermented bean dregs, drying and crushing to obtain the natto bacillus feed additive. The feed additive can promote the growth of pig, promote the growth of lactobacillus and inhibit colibacillus.

The invention discloses a microbial deodorizing inoculant which is used in refuse sites, public toilets and livestock / poultry cultivation farms. The microbial deodorizing inoculant contains Candida utilis of which the collection number is ACCC 20060, Lactobacillus plantarum of which the collection number is ACCC 11016, lactic streptococci of which the collection number is ACCC 10653, cerevisiae fermentum of which the collection number is ACCC 21135, Streptococcus thermophilus of which the collection number is ACCC 10651, Bacillus licheniformis of which the collection number is ACCC 01870, and Bacillus natto of which the collection number is ACCC 19833. The microbial deodorizing inoculant absorbs and degrades generated malodorous gas hydrogen sulfide and ammonia gas, eliminates the malodor of the garbage and garbage leachate, inhibits the putrefaction bacteria from growth, basically prevents the malodorous gas from generation, and forms a disease-spread-free refuse piling environment capable of eliminating the malodor.

The invention relates to a cattle and sheep feed, in particular to a method for preparing the cattle and sheep feed by fermenting bagasse. The cattle and sheep feed is prepared from the following raw materials in parts by weight: 70-80 parts of bagasse, 10-15 parts of molasses, 8-12 parts of a fermenting agent, 10-20 parts of food grain feed, 0.5-2 parts of non-protein nitrogen, and 0.5-1.0 part of table salt. The fermenting agent is produced by cultivating and compounding 15%-20% of candida utilis, 8%-14% of bacillus subtilis, 8%-12% of trichoderma viride, 5%-10% of bacillus natto, and 3%-7% of bacillus licheniformis. The technical scheme of the invention that a by-product, i.e., the bagasse, in a sugar refinery is fermented for preparing the cattle and sheep feed belongs to a project for reusing scraps and turning waste into wealth without discharging waste residues, so that the cattle and sheep feed is environment-friendly. The making method is simple, and the cost is low; the cattle and sheep feed which is prepared through fermentation is comprehensive in nutrition, is moderate in fragrant and sweet degrees, and improves the appetite of cattle and sheep. The cattle and the sheep love eating the cattle and sheep feed, and are high in growth speed.

A natto food is prepared from soybean, water, and milk or milk powder or flutinous rice through inoculating natto bacteria, fermenting, inoculating Bulgarian lactobacillus and thermophilic streptococcus, and fermenting. Its advantages are agreeable taste and rich nutrients.

Bacilliis natto NLSSc with the preservation number CGMCC No. 0724 is Gram staining positive, non-acid fast, straight or near straight, (0.6-0.8)x(1.5-2.0) micron sized and aerobic; has mesial and un-swelled spore with the spore number inside one sporange cell not more than one, meso diamino heptane diacid and glycin contained in the cell wall, no characteristic saccharum and peripheral flagellum; and can be grown well in five kinds of natural organic culture medium and can form relatively thick mycoderm and butter fat colored colony with smooth edge in soybean cake powder culture medium. Bacilliis natto can be cultured in culture medium with nitrogen source to obtain thrombus dissolving matter natto kinase and the cultured matter may be extracted to obtain powdered matter as the additive for thrombolytic health food.

The invention discloses a probiotics microbial agent for regulating livestock intestinal flora and a preparation method thereof. The probiotics microbial agent is prepared by using candida utilis, streptococcis faecium, bacillus subtilis, bacillus natto, lactobacillus plantarum and rhodopseudomonas palustris as raw materials, performing the three-stage liquid fermentation, then uniformly mixing the enlarged cultivated fermenting bacteria with a feed wheat bran, performing the solid fermentation, drying at low temperature to obtain the probiotics agent for regulating the livestock intestinal flora structure. The agent can be used for regulating the livestock intestinal flora structure, promoting the growth of the probiotics, inhibiting the growth of the harmful bacteria, substituting the antibiotics, improving the feed utilization rate, obviously reducing the odor of the excrement of the livestock and improving the ecological environment of aquiculture and round environment, and is efficient, non-toxic and no residue. The probiotics microbial agent can be widely applied to a livestock feed additive so as to prevent the livestock intestinal disease and respiratory disease.

The invention provides a microbial fertilizer manufacturing method. Antibiotic fungus residue serves as a base material, and compound microorganisms are adopted to perform solid fermentation on materials, wherein the materials comprise waste rich in plant fiber and the antibiotic fungus residue. The compound microorganisms comprise cellulose decomposition bacteria and one or more of bacillus natto, saccharomyces cereviseae and lactobacillus plantarum. According to the microbial fertilizer manufacturing method, the problem that it is difficult to treat the fermented antibiotic fungus residue in a pharmaceutical factory at present can be solved. Furthermore, beneficial ingredients in the antibiotic fungus residue are can be utilized and converted into a microbial fertilizer, so as to solve the problem that at present, the soil hardening occurs and the fertility declines.

The present invention relates to freeze-dried powder containing bacillus natto and nattokinase and a preparation method thereof, the process of which comprises that: firstly, culture medium is confected and sterilized, wherein, the culture medium components with the weight percentage are 8 percent to 15 percent of soybean flour, 1 percent to 5 percent of sucrose, 0.4 percent to 0.6 percent of NaCl and the water with the rest quantity, the pH value of which is 6.8 to 7.2; secondly, the bacillus natto is inoculated, and the inoculation quantity is 2 percent to 8 percent; thirdly, the bacillus natto after the inoculation is cultured and fermented for 20 to 28 hours under the temperature of 36 DEG C to 38 DEG C; fourthly, fermentation liquid is frozen and dried in vacuum, the glycerin with 1 percent to 7 percent of the weight of the fermentation liquid is used protecting agent to be added into the fermentation liquid, which is frozen under the temperature of subzero 18 DEG C in advance to be frozen and dried in vacuum for 12 to 20 hours and then is resolved under the temperature lower than 30 DEG C. The method has low cost and is in favor of the industrial production. Under the precondition of maintaining the nattokinase activity, the freeze-dried process also holds other nutrient component in the bacillus natto and the fermentation liquid, which reduces the nattokinase purification cost.

A method for preparing a low molecular weight fish peptide through a Bacillus natto fermentation method comprises the following steps: taking fish to mince, adding water, adding maltose, glucose, lactose or sucrose to obtain a mixture; adjusting the pH value of the mixture, sterilizing at high temperature; cooling, inoculating Bacillus natto seed liquor to ferment; and centrifuging fermentation liquor, filtering, concentrating, drying and grinding to obtain a low molecular weight fish peptide product. The method uses fish as the raw material and utilizes the Bacillus natto fermentation technology to obtain the low molecular weight fish peptide. The product in the invention is very soluble in water, is digested and absorbed easily by human body and has various functions of reducing cholesterol in the blood, promoting lipid metabolism, preventing and curing hypertension and the like. The technology in the invention is also suitable for other low value aquatic product and high-grade aquatic product machine leftover pieces, thus fish resources can be fully utilized; and the technology is simple and easy, the investment is less, the cost is low, the effect can be realized fast, the added value is high and the method is suitable for large-scale industrial production.

The invention discloses fully-mixed granular feed for calves and a preparation method thereof. The preparation method comprises the following steps of: weighing alfalfa meal, corn meal, puffing corn meal, puffing soybean, bean pulp, calf complex vitamin trace elements, natto bacillus, yeast cell wall polysaccharide, calcium hydrophosphate, magnesium oxide, sodium bicarbonate, table salt and mountain flour according to a formula, and mixing in a high-efficiency mixing machine for 5 to 10 minutes; and granulating, and packaging in bags, wherein in the requirement on the mixing uniformity of themixing machine is that the coefficient of variation of the uniformity is less than or equal to 5 percent and the granulation requirement of a granulator is that granules are uniform in colors and luster and free from mildew and peculiar smell, contain less than or equal to 4 percent of flour and pulverized by less than or equal to 10 percent. In a feed formula, the alfalfa meal with a certain granularity is added, and is mixed and granulated according to a ratio to ensure the feed intake of coarse feed for the calves and fulfill the aim of stimulating rumen development. The fully-mixed granular feed is comprehensive in nutrition, high in digestibility, convenient to use and good in palatability, and the average daily gain of the calves can reach 1,100 grams / day.

The present invention provides a manufacturing method of freeze-dried natto. The manufacturing method of the present invention comprises the steps of soybeans immersion, cooking, flavoring, fermentation, pre-cooling, and vacuum freeze drying and so on. The freeze-dried natto is dried under the conditions with low temperature and frost. The thrombolytic enzyme-natto kinase will not inactivate. The flavoring agents are added in the process of manufacturing, which can make the freeze-dried natto have a plurality of flavors like salt, delicious, sweet and so on, can improve the growth of bean bacteria, and can make the natto produce much more kinase. In addition, after the natto is frozen and dried, the natto has loosened texture, which can be eaten as food. The freeze-dried natto is also suitable for the old to eat conveniently. The freeze-dried natto is dry product, and can be packed with paper cup or paper wed without polluting the environment.