The invention discloses a fresh-keeping method of fresh mango cores. Cores of mature mangoes contain mango flavonoid components, mango ketonic acid, gallic acid, quercetin, mangiferin and the like, and the components restrain bacteria and alternaria alternate, have high-oxidation-resistant activity and free radical removal biological activity and are widely applied to biopharmaceutical and healthcare products; however, mango cores after flesh is taken away have mango residues and fruit residue fiber, and are required to be cleaned and to keep fresh. According to the invention, firstly, the biological effect of enzyme is utilized so that pectin substances in the mango flesh are decomposed completely, the residual fruit flesh on the mango cores is decomposed, and nutrition ingredients and water required by the growth of micro organisms are reduced; and secondly, the health-promoting effect of EM (effective microorganisms) bacteria is utilized, after an EM original dew solution is adopted for treatment, the effective bacteria of the mango cores are increased, harmful bacteria are reduced or eliminated, thus the fresh-keeping effect is achieved.