34results about How to "Highly pathogenic" patented technology

The invention discloses a negative pressure ambulance used to prevent nosocomial infection, containing car body, hermetic board cabin connected with car body chassis, control device; the hermetic board cabin is consisted of nose functional cabin and medical afterhold; the nose functional cabin is the flow direction of air controlling cell, the medical afterhold contains cabin air inlet chamber, cabin exhaust chamber, also a medical equipment in cabin; the medical afterhold sets closable port; the cabin air inlet chamber of medical afterhold and air mixing chamber of nose functional cabin can be connected, the cabin exhaust chamber of medical afterhold and the air filtration disinfection chamber can be connected. The air flow of hermetic board cabin has three kinds of state, the air in cabin can all realize directional airflow and pressure gradient. This car can be used as normal ambulance, high pathogenic infectious patient ambulance, various diseases checking car, mobile p3 lab, carrier vehicle of biological bacteria danger foe things; it can be asepsis operation car after switching, high infectivity, strong virulence weapon battlefield command, arm carrying car.

The invention discloses a biological weeding organic fertilizer. The biological weeding organic fertilizer is a solid granule prepared through wrapping a base granule with Sclerotium rolfsii Sacc. SC64 mycelia; a mass ratio of the Sclerotium rolfsii Sacc. SC64 mycelia to the base granule s 1.7-2.0:1; and the base granule is prepared from, by mass, 5-45% of an organic fertilizer, 1.3-28.6% of an adhesive, 10-15% of water, and the balance of a solid matrix. The invention also discloses a preparation method of the biological weeding organic fertilizer, and an application of the biological weeding organic fertilizer in control of dicotyledon weeds. The biological weeding organic fertilizer simultaneously has the advantages of a traditional organic fertilizer and a biological herbicide, so garden wastes and crop straws are fully used to realize changing of wastes into valuables and reduce environment pollution caused by straw burning, and the use of chemical fertilizers and pesticides is reduced to enhance the stress resistance of crops and improve the quality of the crops.

The invention discloses a reference product for detecting pathogenic microorganisms of bloodstream infection and a preparation method thereof, comprising the following steps: (1) obtaining human-derived cells and genomic DNAs of pathogenic microorganisms respectively; Genomic DNA fragmentation of source cells and pathogenic microorganisms; (3) Recovery and purification of fragmented DNA; (4) Valuation and mixing of fragmented DNA; (5) Mixing of fragmented DNA and plasma; (6) Detection of bloodstream infection Extraction and quality inspection of the reference product; using the above preparation method to prepare a reference product for the detection of pathogenic microorganisms of bloodstream infection. The source of the reference product is stable, close to natural plasma samples, covering various pathogens, and the value is more accurate. It can evaluate the quality of the detection results of bloodstream infection detection based on high-throughput sequencing, and can be used for different sequencing platforms, detection processes, Comparison of results between sampling methods, and development of technical standards for bloodstream infection detection based on high-throughput sequencing.

The invention relates to a method for identifying the influence of concurrent bacterial infection on the pathogenicity of avian influenza virus infection and a device used therefor. Co-inoculate bacteria that secrete subtilisin-like protease or / and bacteria that secrete tryptase and low-pathogenic avian influenza virus in the CEF culture, so that the bacteria cannot directly contact CPE but the enzymes they secrete can enter the culture medium, continue Cultivate and observe the presence or absence of CPE; according to the appearance of CPE, the cause of a large number of chicken deaths can be identified. The cell in vitro homogeneous culture device used in the method is composed of a cell culture bottle and a bacteria culture room, and the bacteria culture room is a closed structure with a microporous filter membrane with a pore size not greater than 0.22 microns that can communicate with the cell culture bottle. The method of the invention is simple and easy to operate, the identification time is short, the result is accurate and reliable, and the device used is low in price, and has important significance for the detection and prevention of bird flu.

The invention discloses a subtype detection method of influenza A virus, which comprises the following steps: Step 1, designing and synthesizing primers; Step 2, preparing carboxyl magnetic beads connected with magnetic beads and forward primers; Step 3, A Influenza virus RNA extraction; Step 4, RT‑PCR; Step 5, magnetic adsorption and washing; Step 6, detection. The influenza A virus subtype detection method of the present invention can perform high-throughput, rapid, sensitive and specific typing of the influenza A virus subtype, and has good application value.

The invention discloses a multi-gene detection method of Listeria monocytogenes based on a quantum dot / graphene oxide nanometer platform and belongs to the technical field of gene nanometer detection. The multi-gene detection method of Listeria monocytogenes comprises the following steps of: selecting two or more than two genes of the Listeria monocytogenes as target points of genetic detection and designing two pairs or more than two pairs of genetic PCR (Polymerase Chain Reaction) amplimers according to the analysis result of a gene conserved area; amplifying two or more than two genetic special sequences at the same time according to the principle of LATE-PCR to obtain a corresponding single stranded amplification product; then, hybridizing the single stranded amplification product with a quantum dot fluorescence probe; and finally, quenching and removing the non-hybridized quantum dot probe by using graphene oxide. When the target gene does not exist, all quantum dot fluorescence probes are quenched. On the contrary, when the target gene exists, corresponding fluorescent signals are obtained. The detection method has high detection reliability to Listeria monocytogenes.

The invention provides a strain of Beauveria bassiana efficiently parasitizing grubs, a cultivation method of the strain and an application of the Beauveria bassiana strain in preventing and treating southern blueberry grubs. The Beauveria bassiana strain was deposited in the General Microorganism Center (CGMCC) of the China Committee for the Collection of Microbial Cultures (CGMCC) on December 29, 2017. The preservation number is: CGMCC NO.15090. It is specially used to control blueberry grub pests and has strong toxicity. , Insecticidal speed and so on.

A biochip for detecting avain fluenza virus of both human and fowls is prepared as providing activated chip substrate, providing various fluenza virus subset sections to be sample-set, providing quality control protein, carrying out arrangement pattern design of point measurement, making sample-sets of quality control protein and various avain fluenza virus subset sections according to prepared pattern for detecting out antibodies generated by various subsets simultaneously.

The invention relates to riemerella anatipestifer (RA). The collection number of the RA is CGMCC No.6832. The RA is used for preparing an RA inactivated vaccine. The prepared vaccine can prevent occurrence of infectious serositis of duck characterized by neurological symptom, fibrinous pericarditis, perihepatitis and air sacculitis. The number of RA 6832 in the inactivated vaccine is not less than 1*10<8>CFU / mL. The RA obtained by screening has high pathogenicity. The vaccine prepared from the RA can prevent occurrence of infectious serositis of duck characterized by neurological symptom, fibrinous pericarditis, perihepatitis and air sacculitis, and has good market promotion prospects.

A process for preparing the efficient bacterial herbicide includes sampling the soil near the root of weeds such as crabgrass herb, caper euphorbia, cassia, etc. or their stem or leaves, separating with NPC culture medium, and the chlorella pyrenoidosa and glutamine synthetase depressants, and screening the bacterial strains with high herbiciding activity and broad spectrum. Its advantage is high herbiciding effect.