43 results about "Notch signalling pathway" patented technology

The regenerative potential of aged stem cells is enhanced by activation of the Notch signaling pathway and / or inhibition of TGF-β signaling pathway. Stem cells in aged tissues are capable of proliferation and tissue regeneration, but in their native setting are not provided with the appropriate signals to do so. By administering tissue regenerating agents, organ stem / progenitor cells are provided with the appropriate signals to regenerate the corresponding differentiated tissues.

The invention provides application of a Notch signaling pathway inhibitor in preparation of drugs treating autism. Researches of the brain area of an autism animal model find that the Notch signalingpathway of the VPA autism model is more active and protein expression of NICD and downstream target molecule HES-1 is increased; meanwhile, the researches further find that application of DAPT reducesprotein expression of NICD and HES-1 and reduction of activity of the Notch signaling pathway can improve repeated, rigid behaviors of the autism animal model and increase the social contact abilityof the autism animal model so as to improve space learning and memory ability. The Notch signaling pathway inhibitor can reduce the activity of the Notch signaling pathway of an autism mouse model andimprove autism behavior abnormalities, thereby providing experimental basis and theoretical basis for research of drugs treating autism and clinical treatment of autism, and having excellent clinicaltreatment and drug research and development values.

A purification and renaturation method of Notch ligand Delta-like1 fusion protein is characterized by comprising steps as follows: 1), an inclusion body containing HD1R is repeatedly washed and centrifuged by a washing buffer and is dissolved in a strong denaturant buffer to obtain crudely separated HD1R protein; 2), a denatured leaching solution containing human proinsulin is subjected to direct sample introduction to a flowing phase A balanced chromatographic column and is subjected to isocratic elution by a flowing phase B line of PBS (poly butylenes succinate), 1 mol / L of NaCl or Tris and 1 mol / L of NaCl, target peak chromatographic fraction is collected, and renatured and purified HD1R chromatographic fraction is obtained; and 3), the collected target peak chromatographic fraction is subjected to direct sample introduction, is further desalinated by the flowing phase A balanced chromatographic column of 20 mmol / L of PBS and is continuously eluted for 30 min, the elution flow velocity is 0.5-1.5 ml / min, and the renatured and purified chromatographic fraction with higher purity and higher activity is obtained. According to the method, the process is simple, large-scale production can be realized, and the obtained fraction with high yield and high purity can provide a reliable experimental material for intensive study of Notch signal pathways.