Disclosed is a method to recover an
antigen presenting cells (APCs) and immune cells rich mixture (AIM) from
peripheral blood mononuclear cells (PBMC) mobilized with one or more
cell adhesion inhibitors for the preparation of an AIM vaccine or an AIM
adoptive immunotherapy preparation. In addition, AIM mobilization can be enhanced by priming, simultaneously or in sequence, one or more of a combination of different chemical compounds, cytokines, hormones, growth factors, etc. The interaction of chemokines and
chemokine receptors enable
tumor cells attachment or in close proximity to
antigen presenting cells and immune cells which possess similar receptors in a micro niche environment. Severing the
chemokine /
chemokine receptor linkage by a
cell adhesion inhibitor will release these specifically primed
cell mixtures into the
peripheral blood. The collection of these cells from the
peripheral blood has never been described and is the basis of this invention. AIM cells can either be used alone or better still, be induced into more target specific preparations with additions, modifications and incubation, pre or post
cell adhesion inhibitors mobilization, with vaccines, different target specific antigens, peptides, chemotherapeutic agents, oncolytic viral therapeutic agents, cytokines, co-stimulatory molecules, anti-
regulatory T cell therapeutic agents, anti-CTLA4, anti-PD1 molecules and other methodologies of immunological enhancement known to the art. The AIM vaccine or AIM
adoptive immunotherapy preparation can then be used, but not limited to, the treatment of
cancer and other diseases.