106 results about "Select agent" patented technology

We describe a method of selecting an enzyme having replicase activity, the method comprising the steps of: (a) providing a pool of nucleic acids comprising members each encoding a replicase or a variant of the replicase; (b) subdividing the pool of nucleic acids into compartments, such that each compartment comprises a nucleic acid member of the pool together with the replicase or variant encoded by the nucleic acid member; (c) allowing nucleic acid replication to occur; and (d) detecting amplification of the nucleic acid member by the replicase. Methods for selecting agents capable of modulating replicase activity, and for selecting interacting polypeptides are also disclosed.

The invention provides methods for the selection of transgenic cells. The invention relates to the unexpected finding that cells expressing a gene conferring tolerance to auxin-like herbicides such as dicamba may be directly selected from non-transgenic cells using auxin-like herbicides as a selective agent. In this manner, plants exhibiting tolerance to auxin-like herbicides can be directly produced without the need for separate selectable markers.

The present invention provides methods for generating mammalian T cell populations comprising antigen-specific CD4+CD25+ regulatory T cells from freshly isolated CD4+CD25− T cells. The method comprises selecting CD4+CD25− T cells from a sample obtained from a mammalian subject; determining the MHC Class II type of the subject; inducing the generation of antigen-specific regulatory T cells by contacting the isolated CD4+CD25− T cells in a culture vessel with an induction agent for a time period sufficient to generate antigen-specific CD4+CD25+ regulatory T cells; and selecting the CD4+CD25+ antigen-specific regulatory T cells by sorting the cells in the induction culture with a selection agent comprising at least one artificial multimeric MHC Class II / peptide complex that corresponds to the MHC Class II type of the subject.

The present invention provides methods for generating mammalian T cell populations comprising antigen-specific CD4+CD25+ regulatory T cells from freshly isolated CD4+CD25− T cells. The method comprises selecting CD4+CD25− T cells from a sample obtained from a mammalian subject; determining the MHC Class II type of the subject; inducing the generation of antigen-specific regulatory T cells by contacting the isolated CD4+CD25− T cells in a culture vessel with an induction agent for a time period sufficient to generate antigen-specific CD4+CD25+ regulatory T cells; and selecting the CD4+CD25+ antigen-specific regulatory T cells by sorting the cells in the induction culture with a selection agent comprising at least one artificial multimeric MHC Class II / peptide complex that corresponds to the MHC Class II type of the subject.

The present invention discloses and claims an apparatus and method for delivering a wide array of selected agents to a target from stand-off distances. The apparatus includes a self-destruct frangible projectile made of primary, binding, and active components. The primary component generally has a specific gravity greater than lead, and the binding component generally has a specific gravity less than lead. The active component may be a metal for penetrating the target, a dye for optically marking the target, or another specially selected agent for delivery to the target, depending on the particular embodiment. The frangible projectile self-destructs upon contact with the target, in proximity to the target, or after passing the target, depending on the particular embodiment.

The invention relates to an IP restricted controlled source information capture method based on agent pools, and belongs to the field of data acquisition and processing in the computer science. The method comprises the following steps: maintaining two agent pools A and B, and storing all agents in the agent pool A; testing available agents and initializing the agent pool B; randomly selecting agents in the agent pool B for use in a capture process; triggering an updating operation of the agent pool B by a dynamic maintenance operation of the agent pool B in the capture process; and realizing efficient capture of an IP restricted controlled information source by using the agent pool algorithm. According to the IP restricted controlled source information capture method, the two agent pools are maintained, the available agents are dynamically regulated and controlled in the information capture process to solve the problems of instable agent and low capture efficiency and expand resources for all kinds of data drive experiments.

The present invention provides for methods and compositions for transporting agents and macromolecules across biological membranes. In one embodiment, the invention relates to a method for enhancing transport of a selected agent across a biological membrane, wherein a biological membrane is contacted with a composition containing a biologically active rotaxane capable of selectively transporting the selected agent. The host-rotaxane is effective to impart to the agent an amount transport and / or rate of trans-membrane transport across a biological membrane that is greater than the amount and / or rate of trans-membrane transport of the agent without the host-rotaxane.

The invention provides methods for the selection of transgenic cells. The invention relates to the unexpected finding that cells expressing a gene conferring tolerance to auxin-like herbicides such as dicamba may be directly selected from non-transgenic cells using auxin-like herbicides as a selective agent. In this manner, plants exhibiting tolerance to auxin-like herbicides can be directly produced without the need for separate selectable markers.

Systems and methods are disclosed herein for facilitating shopping assistance. A shopping list is posted by a principal and presented to a plurality of potential agents. Responses are received from the potential agents and a response manually or automatically selected. Partial access to a shopping account of the principal is provided to the selected agent. Partial access may enable the agent to view a shopping list generated by the agent and to associate transactions of the agent with the principal. At a point of sale (POS) an agent may be authenticated and enabled to select a principal with which to associate a transaction. Real time data may be transmitted to a principal as items of a shopping list are checked off by the agent and updates and feedback from the principal may be provided to the agent.

The present invention provides a method of detecting thermonuclease-positive staphylococci that does not require inactivation of DNase-positive / TNase-negative bacteria with heat. The method includes (a) providing a culture medium selective for growing staphylococci; (b) inoculating the culture medium with a sample; (c) incubating the inoculated culture medium under conditions effective to promote the growth of staphylococci; (d) providing an indicator system that produces a differentiable, detectable signal in the presence of thermonuclease-positive staphylococci; (e) contacting the indicator system with the inoculated, incubated culture medium, thereby forming a detection assembly; (f) incubating the detection assembly under conditions effective for generating the differentiable, detectable signal; and (g) detecting the detectable signal.The present invention also provides a culture medium for the selective identification of Staphylococcus aureus. The culture medium includes at least one first selective agent that selects for growth of staphylococci; at least one second selective agent for differentiating Staphylococcus aureus from other staphylococci; at least one first indicator for indicating the presence of staphylococci; and at least one second indicator for differentially indicating the presence of non-staphylococci bacteria. The present invention also provides a method of selectively identifying Staphylococcus aureus in a sample by using the culture medium of the present invention.

A method and a system for the creation of agents to be used for recommending media content to a user (207). The agent comprises a profile of media content and can further comprise an algorithm for recommending media (201) on a media system (208). The media system (208) can be a VCR, a TV, a set-top box, a DVD player, a radio or a personal computer. The method includes the steps of validating an agent in an off-line system (210), transferring agents forth and back between the off-line system (210) and an on-line system (203). The step of validating includes the steps of composing a test set of historical content, estimating scores of an agent in the test set, determine average difference between agent scores and historical relevance feedback, determining performance of an agent using average difference of all agents over the test set and reliability of historical relevance feedback. The method further includes the steps of nominating agents in the on-line system (203), selecting agents and copying agents forth and back between the on-line system (203) and the off-line system (210); importing, generating, training, ranking, nominating and deleting agents in the off-line system (210).

Compositions containing bacteriophages and methods of using bacteriophages in microorganism detection assays and microbial growth and plating media are disclosed. The lytic ability of these phages to control the growth of non-target populations provides superior sensitivity and specificity to detection assays and reduces false negative and false positive results. The removal of contaminating bacteria reduces the microbial competition for nutrients in the growth media thereby increasing the efficiency and productivity of the culture. The phage treatment of the sample increases the proportion of target microorganisms in the sample over contaminating bacteria thereby requiring less time for enrichment to obtain a significant signal improving overall signal to noise ratio in assays and providing for higher yield of end product in microbiological production systems.

The present invention belongs to the field of plant transgene technology, and is especially agrobacterium mediating method of cultivating transgenic plant of sycamore as one garden tree. The agrobacterium mediating method includes the steps of genetic transformation, PCR detection and Southern hybridization detection, and features that through agrobacterium mediation to transduce GUS reporter gene to acceptor sycamore, adding kanamycin as selecting agent to the basic culture medium, screening the transformed acceptor cell, PCR detection, GUS gene detection, Southern hybridization and other steps, transgenic plant of sycamore is obtained. The present invention provides technological platform for the genetic improvement of tree, especially sycamore.

The invention relates to a method for producing a transformed eukaryotic host cell which expresses a recombinant polypeptide of interest which method comprises introducing into a eukaryotic host cell: (a) a first polynucleotide vector which comprises (i) a first nucleotide sequence which encodes a recombinant polypeptide of interest, and (ii) a second nucleotide sequence encoding a selectable marker, which second nucleotide sequence is amplified when the host cell is contacted with a selection agent; and (b) a second polynucleotide vector having essentially the same nucleotide sequence as the first polynucleotide vector except that the second nucleotide sequence is replaced with a third nucleotide sequence which encodes a different selectable marker; the first polynucleotide vector and second polynucleotide vector being stably integrated into the genome of the host cell.

A method is disclosed for producing a transgenic cotton plant comprising the steps of (a) obtaining cottonfibrous root explants, (b) culturing the fibrous root explants to induce callus formation, (c) exposing root callus to a culture of Agrobacterium tumefaciens that harbors a vector comprising an exogenous gene and a selectable marker, the Agrobacterium being capable of effecting the stable transfer of the exogenous gene and selection agent resistance gene to the genome of the cells of the explant, (d) culturing the callus in the presence of the selection agent to which the selection agent resistance gene confers resistance so as to select for transformed cells, (e) inducing somatic embryo formation in the selected callus culture, and (f) regenerating the induced somatic embryos into whole transgenic cotton plants.

A method of producing a transgenic monocotyledonous plant includes culturing a thin section explant from a monocotyledonous plant, such as sugarcane, wheat or sorghum, in the presence of an auxin and, optionally, a cytokinin, prior to transformation. Optimally, the thin section is oriented during this pre-transformation culture period of 1-6 days so that a basal surface is substantially not in contact with the culture medium. The cultured explant is then transformed followed by a rest period of 4-15 days in a culture medium without selection agent but comprising an auxin and, optionally, a cytokinin. After this rest period, transgenic plants are selectively propagated from the transformed plant tissue in the presence of a selection agent such as paromomycin sulphate or geneticin. This system provides rapid, efficient generation of transgenic monocotyledonous plants from transformed, non-callus tissue and thereby reduces the likelihood of somaclonal variation among transgenic progeny. Favorable examination of the present application is respectfully requested at this time.

This invention provides methods for preventing or treating infection by viruses, in particular an influenza virus by modulating transporters, carriers, and ion channels. Methods to identify, validate, and classify the cellular proteins required by viruses during infection of host cells in order to select agents which can inhibit viral infection are described herein. The method employs a siRNA screening platform and uses gene silencing to map the ‘viral infectome’—a compilation of cellular proteins that the virus needs to establish infection and drive the infectious cycle. Charting the infectome provides information on the viral biology by the identification of host cell proteins involved in viral infection and allows the development of novel anti-viral drugs that prevent the viruses from establishing productive infection in cells.

The invention belongs to the technical field of organic synthesis and provides a method for preparing caspofungin. The preparation method of the caspofungin comprises the following steps: a) reacting a compound II with phenylboronic acid and a stereochemical structure selecting agent so as to generate a compound III, wherein the stereochemical structure selecting agent is 2-sulfydryl benzothiazole or 1-phenyl-5-sulfydryl-tetrazole; b) reducing the compound III into amine, namely, a compound IV; and c) finally reacting the compound IV with quadrol for substitution, so as to obtain a target compound. According to the preparation method of the caspofungin, the non-toxic stereochemical structure selecting agent is adopted, so that the preparation process is safer and the preparation method is more suitable for industrial production.

The apparatus and method provide assistance to an agent in a communication system, such as a communication system having an automatic call distributor. The method has the steps of determining skills of each agent of a plurality of agents, storing the determined skills for respective agents of the plurality of agents as skill records in a database, retrieving the skill record of a predetermined agent from the database, comparing the skill record of the predetermined agent to the skill records of the other agents in the database, selecting an agent from the plurality of agents based on the comparison of skill records, and establishing communication between the predetermined agent and the selected agent. The skill record of a predetermined agent is retrieved when the predetermined agent of the plurality of agents requests to communicate with another of the agents of the plurality of agents. The apparatus implements the method. The method and apparatus provide the ability for an agent or an inbound caller to solicit assistance from a group of similarly or higher skilled agents, especially in remote environments where, in the case of an agent, the agent does not have direct contact with other agents.

The invention discloses a pesticide and fertilizer combination special for late winter and early spring facility cultivation for early-maturing eggplants and an application method, and belongs to the technical field of agriculture. The pesticide and fertilizer combination special for late winter and early spring facility cultivation for the early-maturing eggplants is prepared from 2182-5353 parts of base pesticide fertilizer, 350-1600 parts of field planting agent, 61-129 parts of seedling strengthening pesticide fertilizer, 3-5 parts of flower dipping agent and 265-516 parts of fruit strengthening pesticide fertilizer. The pesticide and fertilizer combination for the eggplants can provide needed nutrition scientifically and reasonably according to the growth law of the eggplants, and inhibit occurrence and spread of disease before eggplant plants are attacked or during the early stage when the plants are attacked, loss caused by the disease is reduced, and the pesticide fertilizer needed during the whole growth reason of the eggplants can be met. The selected agents are chemical or biological reagents low in toxicity or free of toxicity and residual, which is beneficial for protecting environment.

High-throughput screening method and apparatus arm described. The method includes placing cells on a substrate defining a plurality of discrete microwells, at a well density of greater than about 100 / cm2, with the number of cells in each well being less than about 1000, and where the cells in each well have been exposed to a selected agent. The change in conductance in each well is determined by applying a low-voltage, AC signal across a pair of electrodes placed in that well, and synchronously measuring the conductance across the electrodes, to monitor the level of growth or metabolic activity of cells contained in each well. Also disclosed is an apparatus for carrying out the screening method.

This invention provides methods for inhibiting or treating infection by viruses, in particular pox viruses by modulating a kinase, in particular by inhibiting a host cell kinase, involved in mediating viral infection. Methods to identify, validate, and classify the cellular proteins required by viruses during infection of host cells in order to select agents which can inhibit viral infection are described herein. Using a systems biology approach the virus / host cell interaction is studied from initial attachment of the incoming virus to the cell surface, to entry, transcription, replication, biosynthesis, and assembly of progeny particles. The method employs a siRNA screening platform and uses gene silencing to map the ‘viral infectome’—a compilation of cellular proteins that the virus needs to establish infection and drive the infectious cycle. Charting the infectome provides information on the viral biology by the identification of host cell proteins involved in viral infection and allows the development of novel anti-viral drugs that prevent the viruses from establishing productive infection in cells.

The invention relates to Id knockout mammals having a disruption in at least one and at most three alleles of inhibitor of differentiation genes, Id1 and Id3. This results in reduction or prevention of a cell proliferative disorder in the mammal as compared to a wild-type mammal. In particular, tumor growth and / or metastasis is shown to be inhibited. Further, tumor growth is shown to have poor vascularization and extensive necrosis in Id knockout mammals lacking 3 out of 4 of the Id1, Id3 alleles (Id1− / −Id3±). Drug screening methods to select agents useful to affect activity or expression of Id1 or Id3 gene products are disclosed. Therapeutic methods employing selected agents in subjects in need of treatment and diagnostic methods and test kits to identify subjects having, or at risk of having, a neurological or cell proliferative disorder are also described.

The invention discloses a gene and its protein of phosphomannose isomerase, and its application. The purpose is that providing a gene and its protein of phoshomannose isomerase, and applying the gene and protein in selecting trans-genic plant. The gene has one of nucleotide sequences as follow: 1) the SEQ ID NO: 1 DNA sequence in sequence table; 2) coding the DNA sequence of SEQ ID NO: 2 in the sequence table; 3) the nucleotide sequence that can hybrid with restrict DNA sequence of SEQ ID NO: 1 in the sequence table at high strict condition. Mannose has no harm for human and animal, degradation-liable in environment and selected marker system has the advantages of product yield safety, the selecting regent price is cheap, the selecting procedure is simplicity and the selected marker system don't impact the metabolism balance of transformation plant, the trans-genic plant grow vigorous, significance effect selection. The selected marker system has potential and extensive application outlook in trans-genic plant study and its market process; it may be the leading selected marker system in plant transformation.