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87 results about "Cellular proteins" patented technology

Updated December 14, 2018. Proteins are very important biological molecules in cells. By weight, proteins are collectively the major component of the dry weight of cells. They can be used for a variety of functions from cellular support to cell signaling and cellular locomotion.

Methods for generating high titer helper-free preparations of released recombinant AAV vectors

This invention provides methods and compositions for producing high titer, substantially purified preparations of recombinant adeno-associated virus (AAV) that can be used as vectors for gene delivery. At the onset of vector production, AAV producer cells of this invention typically comprise one or more AAV packaging genes, an AAV vector comprising a heterologous (i.e. non-AAV) transgene of interest, and a helper virus such as an adenovirus. The AAV vector preparations produced are generally replication incompetent but are capable of mediating delivery of a transgene of interest (such as a therapeutic gene) to any of a wide variety of tissues and cells. The AAV vector preparations produced according to this invention are also substantially free of helper virus as well as helper viral and cellular proteins and other contaminants. The invention described herein provides methods of producing rAAV particles by culturing producer cells under conditions, such as temperature and pH, that promote release of virus. Also provided is a quantitative, high-throughput assay useful in the assessment of viral infectivity and replication, as well as in the screening of agent that affect viral infectivity and / or replication.
Owner:TARGETED GENETICS CORPORTION

Sample pretreatment method based on flow combination ICP-MS (Inductively Coupled Plasma Mass Spectrometry) single cell protein detection

The invention discloses a sample pretreatment method based on flow combination ICP-MS (Inductively Coupled Plasma Mass Spectrometry) single cell protein detection, and belongs to the technical field of sample pretreatment of flow cytometry. The sample pretreatment method comprises the following steps: (1) collecting and transporting a whole blood sample; (2) performing PBMC (Peripheral Blood Mononuclear Cell) cell separation; (3) performing cell active dyeing; (4) performing cell stimulation; (5) performing cell immobilization; (6) performing surface antibody dyeing; (7) performing intracellular phosphorylated protein dyeing; (8) performing single cell labeling, and the like. With the combination of a metal element labeled antibody with cell surface antigen, labeled cells are mixed with beads as internal reference for standardization, dead cells and living cells are distinguished in the pretreatment process, then the situation that the testing result analysis and description are affected by too many dead cells is avoided, single cells are distinguished from cell dimmers, cell trimers or even cell multimers, and flow combination ICP-MS single cell protein detection requirements can be very well met.
Owner:马鞍山普梅森医学检验实验室有限公司

Promotion of Cell Migration and Axon Regeneration in the Cns

The present invention relates broadly to methods for stimulating neural progenitor cell migration to certain regions of the nervous system where the neural progenitor cell naturally would not migrate. These methods have wide interest in fields related to the development of therapeutic approaches for addressing a broad range of neurodegenerative and demyelinating pathologies of the central nervous system. In order to accomplish these various outcomes, the invention provides modalities for introducing into a cell of the CNS a substance that promotes polysialylation of a protein component of the cell. This process underlies various methods of the invention, such as a method of polysialylating a protein of the cell, a method of promoting migration of a neural progenitor cell from a first region of a brain to a second region of the brain, or a method of promoting a neural progenitor cell originating in a first region of a brain to differentiate in a second region of the brain. The methods described above provide the basis for various therapeutic methods disclosed in the invention. In one example a method is disclosed of inhibiting the development of, treating, or ameliorating a neurological pathology in a subject, wherein the method includes introducing into brain cells, located in a path starting in a location close to where neural progenitor cells are located and ending in an area of the CNS where it is desirable that the neural progenitor cells migrate to, of the subject a substance that promotes polysialyltransferase activity that polysialylates a protein of the cells. In a second example a method is disclosed of inhibiting the development of, treating, or ameliorating a neurological pathology in a subject, including administering to the subject a substance that promotes polysialyltransferase activity in an amount effective to treat the pathology.
Owner:MEMORIAL SLOAN KETTERING CANCER CENT

Cell based signal generation

The present invention makes available a rapid, reproducible, robust assay system for screening and identifying pharmaceutically effective compounds that specifically interact with and modulate the activity of a cellular protein, e.g., a receptor or ion channel. The subject assay enables rapid screening of large numbers of compounds to identify those which act as an agonist or antagonist to the bioactivity of the cellular protein. In particular, the assay of the invention makes use of a cell that harbors a protein that is responsive to a cellular signal transduction pathway. The protein is operatively linked to a polypeptide which causes a detectable signal to be generated upon stimulation of the pathway, e.g., when a compound interacts with and modulates the activity of a cellular receptor or ion channel of the cell. Thus, the cell provides a signal generation means comprising a novel fusion protein the expression of which is independent of stimulation / activation of the signal transduction pathway, but the activity of which is responsive to the signal transduction pathway.
Owner:CADUS TECH

Method for preparing hydrolyzed oligosaccharides and short peptides from salt algae residues

The invention provides a method for preparing hydrolyzed oligosaccharides and short peptides from salt algae residues, which belongs to the technical field of salt algae utilization. The method adopts beta-carotene removed salt algae residues as raw materials, separation and enzymatic hydrolysis are repeatedly performed, and then various products are made after concentration and drying. The method provided by the invention comprehensively utilizes the salt algae residues to make various daily use chemicals, medicines and health products, such as skin energy activating solution, hydrolyzed salt algae oligosaccharides and polysaccharide and short peptides. The residue and precipitate of the hydrolyzed salt algae is retained, but not discarded during production process, and then is used as fermentation base material for microbial fertilizer preparation. The products prepared through the method can be wildly applied to products for skin care, moisture preservation, nutrition, antioxidation and anti-ageing, can be applied to health products and anti-tumor products, and also can be served as single cell protein products to complement nitrogen nutrition and radioprotective products.
Owner:CHONGQING UNIV
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