107 results about "Cellular protein" patented technology

Conjugated nitro alkene compounds hamper or prevent proliferation of cancer cells in cell culture and in cancer patients, which can result in a decrease in tumor size and / or disappearance of the cancer. The compounds may act by interference with cancer cell biochemistry, in which isoprenoid groups such as farnesyl and geranylgeranyl become bonded to various oncogenic proteins such as Ras, RhoA, RhoB, or some other growth-related cellular protein(s).

Presented is a therapeutic method to treat prostate carcinomas in mammals comprising the administration of cellular PAcP protein. Also presented is a method to diagnose androgen-insensitive prostate carcinomas by determining the expression level of cellular PAcP in the prostate carcinomas, a decrease in expression being indicative of androgen-insensitivity. A promoter region that is specifically expressed in prostate tissue is presented, as is a xenograft animal model that mimics human prostate carcinomas in the expression of cellular PAcP.

The present invention relates to multi-phase protein separation methods capable of resolving and characterizing large numbers of cellular proteins, including methods for efficiently facilitating the transfer of protein samples between separation phases. In particular, the present invention provides systems and methods for the generation of multi-dimensional protein maps. The present invention thus provides improved methods for the analysis of samples containing large numbers of proteins.

The invention relates to the synthetic gene coding for hG-CSF which enables expression in E. coli with an improved expression level of the recombinant hG-CSF regarding the total cellular proteins after expression.

A method and kit for simultaneous detection and / or determination of a plurality of modified proteins in a sample. The method comprises: a) contacting the sample under mild protein denaturation conditions with a plurality of first antibodies capable of binding to a specific target protein, the first antibodies being immobilized on solid support material, each first antibody being differentiable from others by a differentiation parameter, whereby the first antibodies bind to respective target proteins present in the sample; b) removing unbound materials from the locus of the first antibodies; c) contacting the materials from step (b) with one or more second antibodies, each of which is specific to a class or subclass of modified proteins or with a plurality of second antibodies, each of which is specific to a modified protein, so as to bind the second antibody or antibodies to modified proteins in the sample; and d) detecting and / or determining a plurality of modified proteins in the sample. The kit comprises a plurality of primary antibodies immobilized on solid support material, one or more buffers for lysing and for washing cellular material samples to be assayed, an assay buffer for conducting the assay, the buffer containing a sulfate or sulfonate detergent, and one or more second antibodies specific to the modified proteins.