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Method for inducing migration of adult stem cells derived from adipose tissue

A technology of adult stem cells and adipose stem cells, applied in animal cells, vertebrate cells, artificial cell constructs, etc.

Inactive Publication Date: 2012-09-12
RNL BIO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There have been some reports on induction of bone marrow-derived stem cell migration by chemokine treatment (Adriana Lopez Ponte et al., Stem Cells, 25:1737-1745, 2007; Marek Honczarenko et al., Stem Cells, 24:1031- 1041, 2006; Sordi V et al., Blood, 106:419-427, 2005; Fiedler J et al., J Cell Biochem, 87:305-312, 2002; Forte G et al., Stem Cells, 24:23 -33, 2006; Wright DE et al., Blood, 87:4100-4108, 1996; Son BR et al., Stem Cells, 24:1254-1264, 2006), but there is no report on the migration of adipose stem cells

Method used

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  • Method for inducing migration of adult stem cells derived from adipose tissue
  • Method for inducing migration of adult stem cells derived from adipose tissue
  • Method for inducing migration of adult stem cells derived from adipose tissue

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0094] Example 1: Isolation of human adipose tissue-derived mesenchymal stem cells

[0095] Human adipose tissue was isolated from abdominal fat by liposuction and washed with PBS. The isolated tissue was finely dissected and then digested with DMEM medium supplemented with collagenase type 1 (1 mg / ml) at 37°C for 2 hours. After washing with PBS, the digested tissue was centrifuged at 1000 rpm for 5 minutes. The supernatant was aspirated off, and the pellet remaining at the bottom was washed with PBS, and then centrifuged at 1000 rpm for 5 minutes. Remaining cells were filtered through a 100-μm mesh filter to remove debris, cells were washed with PBS and then cultured overnight in DMEM (10% FBS, 2 mM NAC, 0.2 mM ascorbic acid).

[0096] Subsequently, non-attached cells were removed by washing with PBS, and the remaining cells were subcultured, while every two days in Keratinocyte-SFM medium (containing 5% FBS, 2mM NAC, 0.2mM ascorbic acid, 0.09mM calcium, 5ng / ml rEGF, 5 μ...

Embodiment 2

[0097] Example 2: Inducing Migration of Adipose Stem Cells

[0098] 2-1: Induction of cell migration by chemokines or growth factors shown in Table 2

[0099] The adipose tissue-derived pluripotent mesenchymal stem cells isolated in Example 1 were mixed with 2×10 4 Cells were seeded at a concentration of 200 μl into each well and induced to migrate by the chemokines or growth factors shown in Table 2 below. 30% FBS was used as the positive control group.

[0100] Table 2

[0101] FBS 30%

BCA-1

TGF-β1

Rantes

CXCL16

IGF-1

MCP-1

EGF

PDGF-AB

MIP-3β

b-FGF

TNF-α

SDF-1α

HGF

[0102] figure 1Results for inducing cell migration are shown. Cells induced to migrate by culture medium were used as negative control group, and cells induced to migrate by 30% FBS were used as positive control group. The result of inducing cell migration was calculated as the relative percentage of 100% negative control...

Embodiment 3

[0108] Example 3: Migration of pretreated adipose stem cells with chemokines or growth factors

[0109] The adipose tissue-derived multipotent mesenchymal stem cells isolated in Example 1 were pretreated with the chemokines or growth factors shown in Table 3 below for 24 hours. The pretreated cells were divided into 2×10 4 The concentration of cells / 200 μl was seeded into each well, and the migration was induced by 10% FBS to observe the difference from the induction of cell migration by 30% FBS. At the same time, untreated adipose-derived multipotent mesenchymal stem cells (untreated cells) were used as a negative control group.

[0110] table 3

[0111] Rantes

[0112] image 3 Results for inducing cell migration are shown. From image 3 The indicated numbers of migrated cells can be seen in these adipose tissue-derived mesenchymal stem cells (AdMSCs) pre-treated with different chemokines or growth factors, which were actively induced by 10% FBS and induced ...

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Abstract

The present invention relates to cell migration capacity of adult stem cells derived from adipose tissue, and more specifically, to treatment of adipose-derived, adult stem cells with cellular protein secretions. In particular, the present invention is a new use for adipose stem cells, whereby pretreatment of adipose-derived, adult stem cells with a specific chemokine or growth factor induces a more effective migration to diseased parts of the body. Adipose-derived adult stem cells, pretreated with specific chemokine or growth factor may be intravenously administered as a simple way to target stem cells (targetting) to the site of the disease and represents potential for cell therapy.

Description

technical field [0001] The present invention relates to the ability of adipose tissue-derived adult stem cells to migrate, and in particular to novel uses of adipose tissue-derived adult stem cells and their specific secreted products, which have the ability to migrate to The ability of a disease site in which a chemokine or growth factor is expressed, and the present invention relates to a method for more effectively enhancing the function of a chemokine or growth factor receptor. Background technique [0002] Stem cells refer to cells that not only have self-replication ability, but also have the ability to differentiate into at least two types of cells, and can be divided into totipotent stem cells, pluripotent stem cells (pluripotent stem cells) and multipotent stem cells (multipotent stem cells, multipotent stem cells) ). [0003] Totipotent stem cells are cells with totipotent properties capable of developing into a complete individual, which are possessed by cells up...

Claims

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Application Information

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IPC IPC(8): C12N5/02C12N5/071C12Q1/02
CPCA61K35/12A61P29/00A61P31/00A61P43/00C12N5/0667C12N2501/21
Inventor 罗廷灿姜成根白善轸
Owner RNL BIO
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