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High efficiency biological weed control bacterial and breeding selection method

A herbaceous fungus and biological technology, which is applied in the field of biological herbicide Xanthomonas campestris pathogenic species of Amaranth reciprocatum and its breeding field, can solve the problems of plant leaf necrosis, infection, plant dwarfing, etc., and achieve low cost, The effect of simple strain and easy use

Inactive Publication Date: 2005-11-23
ZHEJIANG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The phytotoxin phaseolotoxin produced by Pseudomonas syringae pv. phaseolicola can make the leaves of Kudzu appear chlorosis and cause local necrosis; Green, serious cause necrosis of plant leaves

Method used

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  • High efficiency biological weed control bacterial and breeding selection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1: Amaranthus retroflexus

[0027] Spray fungal agents at the stage of 4 true leaves from the budding of Amaranthus retroflexus seeds, and the dose is 500L / ha, which shows a good control effect (see Table 3). New leaves wilted after 1 day, old leaves began to wilt after 2 days, leaves basically withered after 4 days, and the control effect reached more than 90%. After 3 weeks, the control effect decreased slightly, and part of the stems and leaves of Amaranthus reflexae began to recover, but the growth was poor, and it had no effect on the protected plants.

Embodiment 2

[0028] Example 2: Cassia

[0029] Spraying fungicides at the stage of cassia seed germination to the fourth round of true leaves, the dose is 500L / ha, shows a good control effect. After 2 days, the new leaves began to wilt, and after 3 days, some old leaves began to wilt, and after 7 days, the leaves basically withered, and the control effect reached more than 85%. After 3 weeks, the control effect declined, and a small part of the stems and leaves of Cassia Cinnamomi began to recover, but the growth was extremely poor, and did not affect the protected plants.

Embodiment 3

[0030] Example 3: Small quinoa

[0031] The antibacterial agent was sprayed at the stage of budding and 4 true leaves of the seeds of quinoa, with a dose of 500L / ha, and the control effect was good. After 5 days, the leaves basically withered, and the control effect reached more than 85%. After 3 weeks, the control effect decreased slightly, but it had been controlled below the economic threshold.

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Abstract

An efficient bioherbiciding xanthomonas campestris pv. retroflexus is prepared through sampling the soil near the root of weeds such as crabgrass herb, caper euphorbia, cassia, etc and their stem and leaves, separating with NPC culture medium and chlorella pyrenoidosa and glutamine synthetase depressants, and screening the bacterial strains with high herbiciding activity and broad spectrum. Its advantage is high herbiciding effect.

Description

technical field [0001] The invention relates to a biological herbicide Xanthomonas campestris pv. retroflexus and a breeding method thereof. Background technique [0002] Chemical herbicides can effectively control many harmful weeds, but their extensive use has also caused a series of environmental problems, and the development of new chemical herbicides is difficult, with low success rate and high cost. In contrast, microbial herbicides have low toxicity, easy degradation, and good environmental compatibility, and have great development potential. [0003] The research on microbial herbicides began in the 1960s, and a number of commercialized fungal herbicides were launched successively. my country’s Shandong Academy of Agricultural Sciences developed the culture of “Lubao No. 1” Cuscuta spores to control soybean fields. Cuscuta: These fungal herbicides are all spore preparations, with strict requirements for action conditions, high requirements i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/00
Inventor 李永泉庄晓峰孙自玲徐凌李明智
Owner ZHEJIANG UNIV
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