151 results about "Riemerella anatipestifer" patented technology

The invention relates to a riemerella anatipestifer antibody indirect ELISA method detection kit and an application thereof; the kit comprises: a) an antibody detection plate, b) enzyme conjugate working fluid, c) a positive control, d) a negative control, e) a sample diluent, f) 10X concentrated washing liquid, g) a substrate coloured solution A, h) a substrate coloured solution B, and i) a stopping solution; the beneficial and positive effects of the invention are that: the kit is simple in operation; the requirements for apparatuses needed are not high; the kit can be operated by everyone, can meet the requirements with different levels such as epidemic disease monitoring, hygienic epidemic prevention, intensive culture, and individual culture, is suitable for large-scope popularization and application, and has wide market prospects.

The invention belongs to the technical field of biology and particularly relates to a riemerella anatipestifer-escherichia coli shuttle expression vector as well as a construction method and an application thereof. The riemerella anatipestifer-escherichia coli shuttle expression vector disclosed by the invention has a nucleotide sequence of SEQ ID NO:1. The invention further discloses a construction method and the application of the riemerella anatipestifer-escherichia coli shuttle expression vector. The riemerella anatipestifer-escherichia coli shuttle expression vector pRES constructed by the construction method can stably exist and can be copied in riemerella anatipestifer and escherichia coli; an exogenous gene carried by the riemerella anatipestifer-escherichia coli shuttle expression vector can be stably expressed in the riemerella anatipestifer; the riemerella anatipestifer-escherichia coli shuttle expression vector can be used for complementary assay of riemerella anatipestifer deletion mutation strains, research of recombinant riemerella anatipestifer vaccines and the like and has a wide application prospect.

The invention relates to the field of animal medicine, in particular to a method for detecting duck plague virus antibody. The method particularly comprises the steps of the preparation of solid phase antigen, primary antibody combination, secondary antibody combination, developing, detection, judgment and the like. The method is an indirect enzyme-linked immuno sorbent assay (ELISA) method established based on purified recombinant UL51 protein, which has good specificity, and when duck hepatitis virus (DHV), Riemerella anatipestifer (RA), duck Escherichia coli (E.coli) are detected, the detection results are all positive; intra batch or batch-to-batch repeated tests show that variation coefficients are all less than 10%, and duck plague virus (DPV) vaccine immunized duck positive sera diluted at ratio of 1:3200 can be detected.

The invention discloses a riemerella anatipestifer infection attenuated live virus vaccine and a preparation method thereof. The riemerella anatipestifer infection attenuated live virus vaccine disclosed by the invention contains a riemerella anatipestifer GD strain freeze-drying protection agent and a riemerella anatipestifer GD strain is preserved in China Center for Type Culture Collection (CCTCC) on April 1, 2016, with the preservation number of CCTCC NO: M2016161. The riemerella anatipestifer infection attenuated live virus vaccine disclosed by the invention has good safety and does not have any adverse effect on ducks; the riemerella anatipestifer infection attenuated live virus vaccine has a good immune effect, can be used for remarkably improving the resistance of young ducks on riemerella anatipestifer, can be used for clinically preventing riemerella anatipestifer infection and has important economic and social benefits.

The invention discloses a culture medium for production of a riemerella anatipestifer vaccine, which comprises a basic culture medium and an additive. The basic culture medium comprises the following components in percentage: 1.5 to 1.9 percent of tryptone, 0.2 to 0.7 percent of yeast extract, 0.2 to 0.7 percent of beef extract, 0.2 to 0.7 percent of hydrolytic albumin, 0.1 to 0.4 percent of glucose, 0.5 percent of NaCl, 0.1 to 0.7 percent of monopotassium phosphate, 0.5 to 0.9 percent of Na2HPO4.12H2O, 0.1 to 0.2 percent of (NH4)2SO4 and 0.01 to 0.03 percent of NH4Cl, wherein the percentage of each component is weight / volume percentage. The additive is sterilely extracted infected-area strong cattle blood through a jugular vein blood taking or carotid artery blood bleeding method, is subjected to sterile fiber removal, is subjected to freeze thawing at the temperature of 20 DEG C below zero and at room temperature for three times, is preserved for later use at the temperature of 20 DEG C below zero, or is directly preserved at the temperature of 20 DEG C below zero after the blood is extracted and subjected to sterile fiber removal, and is subjected to freeze thawing for three times before use. The culture medium has the advantages of capacities of reducing cost, increasing nutrition, overcoming the defects of complex operation and high labor cost during culture by using live animals or embryos, and use for large-scale fermentation production of the riemerella anatipestifer vaccines.

The invention discloses a virulent phage sensitive to riemerella anatipestifer and application thereof. The virulent phage sensitive to riemerella anatipestifer is named RAP37 and is preserved in China General Microbiological Culture Collection Center with preservation date of March-2-2009 and preservation number of CGMCC No.2908, the diameter thereof is about 1-2mm, the periphery is not provided with halo, the phage is composed of one polyhedral head and lathy tail, the face profile of the head is hexagon and the diameter is about 60nm, the tail is about 200nm long and 8nm wide and the genome thereof is double-stranded DNA. The virulent phage sensitive to riemerella anatipestifer is named RAP37, can decompose the riemerella anatipestifer, and can treat and prevent riemerella anatipestifer disease caused by leading pathogen of the riemerella anatipestifer which endangers duck breeding industry by adopting biotherapy.