221results about How to "Low instrument requirements" patented technology

The invention relates to a method for preparing 6- gingerol and 8- gingerol with fresh ginger. It is characterized in that it compries following steps: cutting fresh ginger into sheets, drying at low temperature, disintegrating, sifting with screen of 100 order, heating and refluxing and extracting with acetic ester or acetone, or extracting through immersion, extracting for three times, combining extract liquid, decompressing and recovering solvent, getting extract concretem dissloving it with acetic ester, treating it with chromatographic silica gel and washing it with benzinum purificatum acetic ester with gradient solvent, checking washing process by using TLC, decompressing to recover solvent and getting coarse 6- gingerol and 8- gingerol; washing coarse product with chloroform- carbinol solvent in Sephadex LH20 gel column, checking washing process with TLC, and getting washung liquid containing 6- gingerol and 8- gingerol with higher purity, decompressing to recover solvent and getting pure 6- gingerol and 8- gingerol, checking content with HPLC. The invention is characterized by simple operation, high productivity and production of two kinds of product at the same time.

A method for electrophoretic deposition preparation of the carbon nanotube film belongs to the field of the nano materials technology. The preparing method comprises the following steps: purification treatment and face finish for the carbon nanotube, the formation of the carbon nanotube stable suspension, electrophoretic deposition for the carbon nanotube film, film drying, and second electrophoretic deposition. The invention has the advantages of simple production equipment, rapid film forming speed, controllable film thickness, and unlimited substrate shape.

The invention discloses a method for preparing graphene by rotationally shearing in an electric field environment. Adopted experimental devices are a reaction container filled with electrolyte, high-purity graphite as a cathode, an inert electrode as an anode and a stirrer. The method comprises the following steps: switching on the cathode and the anode with a direct-current power supply, and stirring the electrolyte by using the stirrer; polarizing the cathode to strip the high-purity graphite under constant voltage, and simultaneously, stirring the electrolyte at a certain speed under the action of the stirrer, so that multi-layer graphene striped from the cathode is subjected to a shearing force again so as to prepare a solution containing graphene; cleaning the solution containing graphene for at least 5 times, ultrasonically dispersing, and centrifuging at a low speed; then, centrifuging a supernatant liquid of a prepared product at a high speed; and finally, drying to obtain graphene. The method disclosed by the invention is simple, convenient, rapid, safe, green and lower in cost; furthermore, natural graphene can be directly striped; due to the method, the traditional technology for preparing graphene by electrochemically striping is improved; and the yield and the quality for preparing a graphene film are increased.

The invention relates to a riemerella anatipestifer antibody indirect ELISA method detection kit and an application thereof; the kit comprises: a) an antibody detection plate, b) enzyme conjugate working fluid, c) a positive control, d) a negative control, e) a sample diluent, f) 10X concentrated washing liquid, g) a substrate coloured solution A, h) a substrate coloured solution B, and i) a stopping solution; the beneficial and positive effects of the invention are that: the kit is simple in operation; the requirements for apparatuses needed are not high; the kit can be operated by everyone, can meet the requirements with different levels such as epidemic disease monitoring, hygienic epidemic prevention, intensive culture, and individual culture, is suitable for large-scope popularization and application, and has wide market prospects.

The invention relates to a primer pairs for rapid detection of toxoplasmosis nucleic acid, a kit and a detection method. The primer pairs for rapid detection of toxoplasmosis nucleic acid comprises aforward primer, a reverse primer and a probe for detecting genome B1 gene sequence of toxoplasmosis. The kit comprises the following contents: a lysate, a diluent, a lyophozyme tube containing the primers, a positive quality control and a negative quality control. The detection includes the process of carrying out a normal-temperature nucleic acid amplification technology by using the kit. Throughmultiple enzymatic reactions of DNA helicase, single-stranded DNA binding protein, DNA polymerase and the like, target DNA can be amplified by millions of times under the condition of constant temperature 37-45 DEG C within 10-30 min; and with the cooperation of the fluorescence detection technique, rapid detection of DNA to be tested can be realized. The detection method of the invention has advantages of simple operation, short time and low requirement on equipment, and is very suitable for rapid diagnosis of pet infectious diseases.

The invention relates to a curcumin solid lipid nanoparticle with a P-gp inhibiting effect, and a preparation method and application thereof. The curcumin solid lipid nanoparticle comprises the following components according to mass ratio: 0.05-1% of curcumin, 5-15% of lipid material, 5-15% of an emulgator and the balance of water. According to the invention, the solid lipid nanoparticle technology is adopted to encapsulate curcumin, so that the stability of medicine is increased, the solubility of medicine is improved, the excretion function of small intestines is reduced, and the bioavailability of curcumin is improved. In addition, the preparation method of the curcumin solid lipid nanoparticle, disclosed by the invention, is the emulsification evaporation and low temperature solidification method which is simple and convenient and suitable for being used in a laboratory, and has low requirements for an apparatus.

The invention relates to a method for electrochemically depositing silver nanoparticles in a titanium dioxide nanotube array. The method comprises the following steps: (a) titanium sheet pretreatment: removing oil stain and impurities on the surface of a titanium sheet, burnishing the surface of the titanium sheet, carrying out polishing treatment, cleaning, and drying for later use; (b) preparing a TiO2 nanotube array on the surface of the titanium sheet by virtue of an anodic oxidation method; (c) deposition of silver nanoparticles: cleaning the titanium sheet on which the titanium dioxide nanotube array is formed on the surface for removing the impurities, then dipping the titanium sheet into mixed liquid of Ag ion-containing ethanol and water, connecting the titanium sheet to a cathode of a power supply, applying a voltage between the titanium sheet processed in the step (a) as an anode and the cathode, and electrochemically depositing silver; and (d) sample treatment: cleaning the sample prepared in the step (c), drying, and calcining. By virtue of the method, Ag nanoparticles can be deposited into a nanotube, so that the SPR effect of the silver nanoparticles can be effectively enhanced, and the photocatalytic performance of the TiO2 nanotube array can be further improved.

The invention relates to a method for preparing magnetic fluid for a ultrahigh vacuum sealing device, which uses the following steps: A) Fe2+ soluble salt and aqueous solution of Fe3+ soluble salt are mixed according to the molar ratio of Fe2+ and Fe3+, which is 1:2. B) obtained mixed solution is placed in 80 DEG C constant temperature water bath, excessive ammonia is added to generate Fe3O4 particles, oleic acid is added, thereby obtaining magnetic nano Fe3O4 particles which are coated with the oleic acid. C) the magnetic nano Fe3O4 particles which are coated with the oleic acid are dispersed in dioctyl sebacate and is placed in 100 DEG C constant temperature water bath, modified polyoxyethylene ether is added after being stirred for 30min, the temperature is increased to 150 DEG C, emulsification pretreatment is done for 60min, the temperature is reduced below 60 DEG C, thereby obtaining the magnetic fluid for the ultrahigh vacuum sealing device. The method for preparing the magnetic fluid for the ultrahigh vacuum sealing device has lower requirements for experiment instruments and operation conditions, the prepared magnetic fluid is very steady, the saturated vapor pressure is very low, and the startup torque is also low.

A method of measuring generation toxicity of medicine and personal nursing products using caenorhabditis elegans, relates to a method of measuring generation toxicity of medicine and personal nursing products (PPCPs) using caenorhabditis elegans. After being contaminated on different concentration gradients and exposure time by synchronized caenorhabditis elegans, life, hatching time, generation time, body size, body corner frequency, inverse motion and olfactory stimulation of caenorhabditis elegans in parental generation P0, first filial generation F1 and second fillial generation F2 are measured, and the result is compared. These indexes characterize toxic effect of PPCPs from different sides, to estimate generation toxicity. The invention is convenient in operation and storage, little in apparatus, provides instruction for measurement and forecast for PPCPs toxicity in real environment from two sides of exposure dose and exposure time, also provides fast estimating approach for procedure of PPCPs, actual effect judgement of bursting event handling, and zoology risk of the novel PPCps.

The invention discloses a nucleic acid aptamer probe and device for cancer cell detection and application of the nucleic acid aptamer probe and device for cancer cell detection. The nucleic acid aptamer probe comprises a fixing probe body and an identifying probe body capable of being combined with a cancer cell; the fixing probe body and the identifying probe body are hybridized and complemented; and the device comprises the nucleic acid aptamer probe and a carrier, and the fixing probe body of the nucleic acid aptamer probe is in cross linkage to the surface of the carrier in a covalent or non-covalent manner. The nucleic acid aptamer probe is low in instrument requirement, high in universality, low in cost, simple in operation and easy to popularize, and can be applied to cancer cell detection, tracing and killing.

The invention discloses a method for preparing a high-yield SiC nanowire. The method comprises the followings steps of: performing mechanical mixing by taking 36.8 to 95.2 mass percent of industrial silicon powder or silicon dioxide powder and 4.8 to 63.2 mass percent of industrial expandable graphite as raw materials; filling a mixture into a graphite crucible and placing the graphite crucible into a high-temperature atmosphere furnace; filling argon as a protective gas after vacuumizing; raising a temperature to be between 1,300 and 1,700 DEG C at a speed of 2 to 15 DEG C / min; keeping the temperature for 1 to 8 hours; keeping the pressure intensity in the furnace lower than 1.0 MPa in an overall preparation process; naturally cooling to room temperature; and opening the furnace to obtain the SiC nanowire. The method has the characteristics of low-cost and easily-obtained raw materials, simple process without using a catalyst and productivity up to 65 percent, and is suitable for industrial production of the SiC nanowire.

The invention provides a method for improving surface bioactivity of polyether ether ketone (PEEK). The method is characterized in that in a PEEK preparation process or a PEEK re-formation process, a porous metal layer with excellent biocompatibility is embedded in the surface of PEEK, thereby obtaining a porous fiber / PEEK composite material; the structure of the composite material is that the surface is a bioactivity coating, and the base is a composite material of the metal porous layer and the PEEK. The method provided by the invention integrates PEEK surface modification and PEEK preparation; the method is simple and has low requirements on instruments. The prepared novel composite material has excellent performances, the bioactivity of the PEEK is improved, bone conduction and body fluid transmission are facilitated, the mechanical property and osteogenesis efficiency are improved, and the prepared composite material has stronger binding force between the porous metal layer and the PEEK so as to meet most application requirements, such as bone filling, bone repair, bone transplantation and bone fixation, and in particular the composite material can be applied to bone transplantation under loading conditions, such as interbody fusion cage, vertebral replacement, femoral head diseases, acetabular cup, fixing bolt and fixing plate.

The invention relates to a lily mottle virus (LMoV) double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) kit and a preparation method. The assay kit comprises a 96 micropore ELISA plate, an enzyme labeled antibody, a buffer solution, a developing solution and a stop solution, wherein the 96 micropore ELISA plate is coated with an LMoV specific polyclonal antibody, and the enzyme labeled antibody is an LMoV specific polyclonal antibody labeled by horse radish peroxidase. An immunogen used in the invention is a fusion recombinant antigen of LMoV coat protein and cytoplasmic inclusion protein, which is expressed by genetic engineering. The double-antibody sandwich enzyme-linked immunosorbent assay established by antibodies produced by the immunization can be used for detecting lily leaf specimens. Compared with other detection methods, the double-antibody sandwich enzyme-linked immunosorbent assay has high detection sensitivity and high accuracy, and ng-level viral antigenscan be detected. The preparation method of the kit is simple, convenient, quick, economical and practical.

The invention discloses a preparation method of Curdlan oligomers. The method comprises the following steps of: fully dissolving Curdlan polysaccharide into a solvent to obtain a homogeneous solution; adding acid at a temperature in a range of 80-140 DEG C, wherein a final concentration of the acid in the solution is 0.1-10 M; reacting for 30-500 min; adding organic solvent sediments which are 3-5 times as much as the volume of reaction liquid and meanwhile, stirring and filtering to obtain a milky solid; washing the solid by the organic solvent 1-4 times to obtain the Curdlan oligomers with an average molecular weight of 340-4000 Da, which is measured by an efficient liquid phase detection after drying. The degradation efficiency of the preparation method reaches more than 85%. The Curdlan oligomers are oligomers formed by connecting glucose molecules through beta-(1->3) glucosidic bonds; the degree of polymerization (Degree of Polymerization, DP) of the Curdlan oligomers is 2-24; a molecular formula of the Curdlan oligomers is (C6H12O6)n, wherein n is equal to 2-24. The average molecular weight of the Curdlan oligomers is 340-4000 Da. The preparation method provided by the invention has a simple and controllable preparation process, short reaction time and good degradation efficiency, and is environmentally friendly and suitable for industrial production.

The invention discloses a stable high-internal-phase Pickering emulsion of modified bacterial cellulose nanofibers and a preparation method of the stable high internal phase Pickering emulsion. The method comprises the following steps: 1) preparing a bacterial cellulose nanofiber solution; 2) dissolving soybean isolate protein into an ethanol solution; 3) adding the solution obtained in the step 1) into the solution obtained in the step 2), adjusting the mass ratio of the bacterial cellulose nanofibers to the soybean isolate protein to be 3:25-9:25, shearing, homogenizing and carrying out rotary evaporation; and 4) mixing the obtained concentrated solution with grease, shearing and emulsifying. According to the preparation method, a large amount of grease is embedded into the bacterial cellulose nanofibers modified by the soybean isolate protein to form the high-internal-phase Pickering emulsion, and the emulsion is high in stability and good in viscoelasticity; the method has low requirements on instruments, is simple and easy to operate, and has a good application prospect in the aspects of nutrient substance conveying carriers and food structure modification base materials.

The invention discloses a colloidal gold chromatography kit for detecting novel coronaviruses (2019-nCoV) and an application thereof. According to the kit, a collected sample is cracked by a cell lysis solution to release pathogen nucleic acid, and then under the action of reverse transcriptase and T7RNA polymerase, amplification of pathogen nucleic acid fragments is realized through reverse transcription and transcription processes. An amplified RNA product is recognized and captured by a specific probe in a detection solution to form an RNA amplification product-specific probe-gold probe compound, and the compound is fixed on an NC membrane through lateral flow chromatography to form a visible strip, so that the detection of the pathogen nucleic acid is realized. The kit has no RNA extraction process, does not need a special instrument, has difficulty in pollution in actual detection based on RNA isothermal amplification, has the advantages of high sensitivity, high specificity and simplicity in operation, and makes wide application of novel coronavirus (2019-nCoV) nucleic acid detection possible.

The invention discloses an infrared-imaging detection method of high-altitude cirrus, and belongs to the field of false-alarm-source and target detection in remote-sensing and infrared-image processing. After an infrared image is acquired, firstly, preprocessing of the image is carried out, and target enhancement processing is carried out on a preprocessing result; then a region growing method isutilized to divide an enhanced portion into mutually disjoint blocks to complete coarse segmentation of the image; then the number of the blocks of coarse segmentation is used as a class number of clustering, clustering centers and a fuzzy membership matrix are initialized, and then positions of pixels relative to a center in the image is used, by a KFCM clustering method, as a basis of determination of an initial membership matrix to carry out clustering iteration to complete fine segmentation of the image; and finally, scattered isolated points / blocks are cleared for completing final segmentation, positions of the cirrus are determined, and a detection result is output. The method solves the problem that according to existing infrared-imaging technology, cirrus is difficult to accuratelydetect, detection efficiency is low, and detection speed is low.

The present invention mainly relates to a method for preparing metal base oxide gauze by utilizing layered layer nano assembly technique. Said method uses the nano oxide glue as adsorption deposition liquor and uses metal gauze as matrix to assemble to assemble to nano oxide on the metal gauze by using layered layer method under the action of static attraction. If the altermative adsorption of different kinds of oxide colloids is utilzied, the heterocomplexed metal base oxide gauze can be obtained. The prepared lattice surface ultrathin oxide film can provide quick diffusion and high active position utilization rate. The high heat conductivity of metal basis is suitable for exothermal reaction, and its unique gauze structure more possesses superiority in mass transfer field, and is convenient for operation. Said invention method can be extensively used in catilisis, separation industry and environmental protection and preparation of sensor and modification of electrode.

The invention belongs to the field of medicines, and relates to an application of a method for degrading all carbohydrate chains in the blood to be monosaccharide and detecting the monosaccharide in cancer detection. A sample in the method for degrading the blood to obtain the monosaccharide and detecting the monosaccharide is the blood, and the cancer includes lung cancer, gastric cancer, ovarian cancer, penis cancer, esophagus cancer, oral cancer, biliary duct cancer, breast cancer, adenocarcinoma perampullaire, rectal cancer and bladder cancer. The method has the characteristics of simplicity and easiness in operation steps, easiness in popularization, short detection time, low requirement on the instrument, low detection cost, less consumption of blood and the like. The result displays that based on the content of eight monosaccharides in the blood, not only can a normal person and a cancer patient be distinguished, but also different cancers can be distinguished. The method for simplifying glycomics knowledge and applying in the detection of the biomarker of blood is first created in the world.

The invention relates to an LIBS and Raman spectrum aerosol online detection device based on a single particle. An aerodynamic lens is used for focusing an atmospheric aerosol particle sample into a collimated particle beam and screening a particle size, and an oscilloscope, two continuous lasers and corresponding photomultipliers jointly form a double-beam diameter measuring system. And a first Nd: YAG laser and a second Nd: YAG laser act with particulate matters to excite a Raman spectrum and an LIBS spectrum. Element composition information in aerosol particles can be detected in real timethrough the LIBS spectrum, characteristic spectral lines of all elements belong to atomic spectral lines, and overlapping interference is avoided. The Raman spectrum studies vibration and rotation spectrum of molecules to obtain structure information of aerosol particles, and obtains lattice vibration information of a particle sample. The device is simple and compact in structure and low in instrument requirement, background noise interference is eliminated only through a low-vacuum environment, and cost of the detection device is reduced. The device is short in detection process, and an analysis result can be obtained after a single pulse laser.

The invention discloses a green and practical method for synthesizing nitrile from aldoxime. According to the invention, oxime is adopted as a raw material. Under the catalysis of a copper-manganese-phosphor composite catalyst, oxime is dehydrated into nitrile. Compared to a traditional synthesizing method, a dehydration agent with equivalent weight is not required, and no harmful waste is produced. The method is simple and is easy to operate. The raw materials are easy to obtain. The method is suitable to be used in medicine synthesizing and productions of fine chemical intermediates.

The invention discloses an H6 subtype avian influenza virus RT-LAMP (reverse transcription loop-mediated isothermal amplification) kit. A reverse transcription loop-mediated isothermal amplification (RT-LAMP) technology is adopted; and six specific primers of LAMP are designed according to eight loci in a conserved region of an H6 subtype AIV (avian influenza virus) hemagglutinin (HA) gene sequence. Due to the application of the H6 subtype avian influenza virus RT-LAMP kit and a detection method established by the invention, the defects of long detection period, low sensitivity, poor specificity, complex operation, high instrument requirement in the prior art are overcome. The H6 subtype avian influenza virus RT-LAMP kit is particularly suitable for the requirement on field detection of the H6 subtype avian influenza virus in the primary-level areas and frontier ports and has important significance for preventing and controlling the H6 subtype avian influenza virus.

The invention relates to a method and a system for supervising fish and quality of a raw meat product of the fish based on DNA (deoxyribonucleic acid) bar code technique and a system. The method includes the steps: (1) extracting DNA of the fish and raw meat product of the fish, and acquiring molecular bar code sequences, namely, mitochondrion COI homologous sequences; (2) performing species identification by a DNA bar code database, converting molecular identification results and product information into a two-dimensional code image, and pasting the two-dimensional code image on the surface of a package; (3) acquiring information such as production places, kinds, processing records and product detection by a consumer by scanning a single two-dimensional code of a commodity through intelligent devices in the circulation process of the fish and raw meat product of the fish. According to the method, automatically supervising of fish products in commercial circulation is achieved by the aid of molecular biology bar code and two-dimensional bar code technology.

The invention discloses a method for testing cable main insulator water tree phenomenon initial points. The invention comprises starting from an angel of a condition that conductible ions are precipitated in a cable semi conductive shielding layer, imitating practical working hot and humid environment of the cable semi conductive shielding layer, characterizing the cable main insulator water tree phenomenon initial points. After sample hot water is cooked, a mathematical model is established, the mathematical model analyzed, a density distribution of the conductible ions which are in a t time sample is obtained, a precipitating condition of the conductible ions in the cable semi conductive shielding layer can be described well, which is used to analyze effects of the cable semi conductive shielding layer to the water tree phenomenon initial points which is produced through a cable main insulating layer. The testing method has the advantages that the requirement for needing equipment is low, tests can be advanced under normal lab conditions, and testing cost can be lowered by 10%. After data base is built successfully, the invention can be moved out immediately if the invention is needed without testing surface smoothness, which saves testing time.

The invention relates to a method for preparing 6- gingerol and 8- gingerol with fresh ginger. It is characterized in that it compries following steps: cutting fresh ginger into sheets, drying at lowtemperature, disintegrating, sifting with screen of 100 order, heating and refluxing and extracting with acetic ester or acetone, or extracting through immersion, extracting for three times, combiningextract liquid, decompressing and recovering solvent, getting extract concretem dissloving it with acetic ester, treating it with chromatographic silica gel and washing it with benzinum purificatum acetic ester with gradient solvent, checking washing process by using TLC, decompressing to recover solvent and getting coarse 6- gingerol and 8- gingerol; washing coarse product with chloroform- carbinol solvent in Sephadex LH20 gel column, checking washing process with TLC, and getting washung liquid containing 6- gingerol and 8- gingerol with higher purity, decompressing to recover solvent and getting pure 6- gingerol and 8- gingerol, checking content with HPLC. The invention is characterized by simple operation, high productivity and production of two kinds of product at the same time.

The invention belongs to the field of medicines, and relates to an application of a method for degrading all carbohydrate chains in the blood to be monosaccharide and detecting the monosaccharide in cancer detection. A sample in the method for degrading the blood to obtain the monosaccharide and detecting the monosaccharide is the blood, and the cancer includes lung cancer, gastric cancer, ovarian cancer, penis cancer, esophagus cancer, oral cancer, biliary duct cancer, breast cancer, adenocarcinoma perampullaire, rectal cancer and bladder cancer. The method has the characteristics of simplicity and easiness in operation steps, easiness in popularization, short detection time, low requirement on the instrument, low detection cost, less consumption of blood and the like. The result displays that based on the content of eight monosaccharides in the blood, not only can a normal person and a cancer patient be distinguished, but also different cancers can be distinguished. The method for simplifying glycomics knowledge and applying in the detection of the biomarker of blood is first created in the world.

The invention provides a phosphorus-doped bimetallic selenide electrocatalyst material with an adjustable monometallic element electronic structure as well as a preparation method and application of the phosphorus-doped bimetallic selenide electrocatalyst material. The bimetallic selenide electrocatalyst material is obtained by carrying out hydrothermal treatment on the surface of foamed nickel to grow nickel-iron hydrotalcite nanosheets and then carrying out selenylation and phosphorus doping. According to the method, introduced phosphorus atoms can independently regulate and control the electronic structure of the metal element iron on the premise that the electronic structure of nickel is not changed, so the electrocatalytic activity of the material is further influenced, and it is indirectly proved that the electronic structure of the element iron has great influence on the catalytic activity. In addition, a material source is wide, a synthesis method is simple and convenient, and requirements on equipment are low; and the obtained material is used for an electrochemical oxygen evolution reaction, and has electrochemical properties such as ultrahigh stability and ultralow overpotential.

The invention discloses a fluorescence probe and a synthetic method thereof and an application of the fluorescence probe in circulating tumor cell detection. The fluorescence probe comprises a fluorescence group, a cathepsin B substrate and folic acid molecules, the fluorescence group is connected with the cathepsin B substrate through a self-degradation bond, and then is connected with the folic acid molecules; the fluorescence group is excited by the excitation light under a dissociation state, and then is enable covalent connection with other molecules for quenching. The fluorescence probe is the switching-mode fluorescence probe having a spontaneous cascade amplification function, can be used for avoiding complex operation steps of antibody or nucleic acid detection during a circulating tumor cell detection process, is used for an automatic biochemical analyzer as a matching reagent, is in favor of promoting automation detection of tumor cells, and has the advantages of simple and convenient operation, short time consuming, and low apparatus requirement. The fluorescence probe can be used for positioning minimal tumor cells and tubercle in clinical operation, and has wide application prospect and large market value.

The invention discloses LAMP (loop-mediated isothermal amplification) primers, a kit and a method for detecting murine klebsiella pneumoniae. A visual detection method of murine klebsiella pneumonia which is suitable for clinical sample detection and has strong specificity and high sensitivity is established by designing the LAMP primers by virtue of a klebsiella pneumoniae tyrB gene sequence. Whether klebsiella pneumoniae exists or not can be detected quickly and accurately by observing whether green fluorescence exists or not, and the lowest detectable limit is 5.6 copy number / reaction. According to identification of a specific sequence region of a target sequence by four primers designed and screened, high specificity of LAMP diffusion is guaranteed, so that the LAMP primers are suitable for field application at basic level.

The invention provides a detection method of transesterification activity of non-aqueous phase lipase. The method comprises the following steps of: taking p-nitrophenol ester as shown in formula (I) and alkyl alcohol as shown in formula (II) as substrates, taking the lipase as a catalyst for performing transesterification in an organic solvent to generate p-nitrophenol and alkyl ester as shown in formula (III), performing organic dissolution and dilution, and then detecting the variation of an ultraviolet absorption value of a reaction solution at 310nm to determine the transesterification activity of the lipase. The beneficial effects of the detection method of the transesterification activity of the non-aqueous phase lipase, disclosed by the invention, are mainly represented as follows: the detection method is simple and feasible, the cost of raw materials is low, the time consumption in detection is low, the requirements for instruments are not high, and the detection method can be used for fast determination of esterification activity of the lipase, and is suitable for being applied to conventional biochemical laboratories.