The invention discloses an automatic detection method and an automatic detection device for a connection wire of an electrical experimental facility. The automatic detection device for the connection wire of the electrical experimental facility comprises a (Micro Processing Unit) MCU, an 8155 chip, 24 relays, 24 free-wheeling diodes, 24 triodes, 48 connection terminals, 24 current-limiting resistors, 24 pull-up resistors, a communication module, an alarming module and a keyboard module. The automatic detection device for the connection wire of the electrical experimental facility adopts the triodes to drive the relays, so that the circuit is simple in structure, the elements are less, the cost is low, the connection status among the terminals of the experimental facility can be automatically detected, the elements are protected effectively, and the reliability is high; the intelligent detection device is adopted, so that the workload of experiment advisors can be effectively reduced; once the detection device emits alarm information, students can find problems by themselves, so that the problem-solving ability of the students is effectively improved, and the quality of teaching is ensured. The automatic detection method and the automatic detection device for the connection wire of the electrical experimental facility are applicable to all electrical experimental facilities for teaching.