Method for detecting generation toxicity of medicament and personal cure by Caenorhabditis elegans

Abstract

A method of measuring generation toxicity of medicine and personal nursing products using caenorhabditis elegans, relates to a method of measuring generation toxicity of medicine and personal nursing products (PPCPs) using caenorhabditis elegans. After being contaminated on different concentration gradients and exposure time by synchronized caenorhabditis elegans, life, hatching time, generation time, body size, body corner frequency, inverse motion and olfactory stimulation of caenorhabditis elegans in parental generation P0, first filial generation F1 and second fillial generation F2 are measured, and the result is compared. These indexes characterize toxic effect of PPCPs from different sides, to estimate generation toxicity. The invention is convenient in operation and storage, little in apparatus, provides instruction for measurement and forecast for PPCPs toxicity in real environment from two sides of exposure dose and exposure time, also provides fast estimating approach for procedure of PPCPs, actual effect judgement of bursting event handling, and zoology risk of the novel PPCps.

Description

technical field [0001] The method for measuring generational toxicity of pharmaceuticals and personal care products by using Caenorhabditis elegans relates to a method for measuring generational toxicity of pharmaceuticals and personal care products (PPCPs) by using Caenorhabditis elegans. It belongs to the technical fields of environmental protection and ecological risk assessment. Background technique [0002] Caenorhabditis elegans belongs to the phylum Nematodes and the class Nematodes. It lives in the soil all over the world, feeds on bacteria, is easy to breed artificially, has no harm to humans, animals and plants, is non-parasitic, and has high safety. It senses smells and tastes and responds to light and temperature. The complete genome sequence of Caenorhabditis elegans was completed at the end of 1998, and it is the first multicellular animal whose complete genome sequence is known. Now, the known nematode genome is 97Mbp, equivalent to 1 / 30 of the human genome....

AI Technical Summary

Problems solved by technology

Although the common acute toxicity test method has the advantage of being fast, it cannot judge the toxic effect of PPCPs in the real environment because the concentration selection is significantly higher than the concentration of PPCPs detected in the environment, so it is impossible to judge the toxicity of the PPCPs in the real environment. Ecological risks arising from PPCPs

Ordinary chronic toxicity, which detects the toxic effects of PPCPs during a certain period of life of the selected test organisms (such as mice, etc.), cannot characterize the effects of PPCPs on organisms, biological offspring and their offspring. Toxic effects of long-term effects on progeny, etc.