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Riemerlla anatipestifer bivalent inactivated vaccine and preparation method thereof

A technology of Ribella and inactivated vaccines, applied in the direction of antibacterial drugs, bacterial antigen components, etc., can solve problems such as difficult to remove, irrational drug regimens, high morbidity and mortality, and achieve saving production and injection costs, health Provides stable development and reduces stress response effects

Inactive Publication Date: 2009-08-19
INST OF ANIMAL HUSBANDRY & VETERINARY FUJIAN ACADEMY OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Riemerellaan atipestifer (RA) disease is currently one of the most serious infectious diseases to the duck industry. It often causes fibrinous pericarditis, perihepatitis, air sac inflammation and caseous salpingitis in ducklings clinically. , conjunctivitis, arthritis, etc., the morbidity and mortality are high. The pathogenic bacteria of the disease is R. anatipestifer. Some strains of this bacteria are sensitive to some antibacterial drugs, but it is difficult to remove them, especially in duck plague. Drugs struggle to reach after Bacillus invades air sacs
At present, due to unreasonable drug regimens, the phenomenon of drug resistance of R. anatipestifer is widespread, and the drug resistance rate is high and widespread. At the same time, it has caused certain problems such as drug residues.
[0003] An important means of preventing and controlling the disease is to inoculate the R. anatipestifer vaccine, but there is currently no commercial vaccine available for the disease in China
Moreover, there are many serotypes of R. anatipestifer, and there are as many as 21 kinds reported in the world, and the cross protection between types is relatively poor, so it brings great difficulties to the prevention and treatment of this disease.

Method used

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  • Riemerlla anatipestifer bivalent inactivated vaccine and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The preparation of embodiment 1 R. anatipestifer inactivated vaccine

[0022] 1. Seed Preparation for Production

[0023] (1) Preparation of first-class seeds: Dissolve the freeze-dried strains of R. anatipestifer in LB medium respectively, inoculate them on tryptone soy agar (TSA) plates containing 2% fetal bovine serum, and place candles Incubate at 37°C for 24 hours in a cylinder. Observe the smooth surface of the bacterial lawn with the naked eye, and select 5 to 10 colonies that are round, slightly protruding, creamy, and slightly blue and translucent;

[0024] The selected colonies were mixed and inoculated with TSA slant containing 2% fetal bovine serum, CO 2 After culturing in an incubator at 37°C for 24 hours, take them out, and observe the pure ones as primary seeds. The primary seeds are stored at 2-8°C.

[0025] (2) Propagation and inspection of secondary seeds: Take primary seeds, wash the bacterial lawn with about 5mL of LB, inoculate in 400mL of LB li...

Embodiment 2

[0036] The preparation of embodiment 2 R. anatipestifer inactivated vaccines

[0037] 1. Seed preparation for production is the same as in Example 1;

[0038] 2. Preparation of bacterial solution for seedling production

[0039] Culture medium is prepared with embodiment 1;

[0040] (1) Bacterial liquid culture: Use a culture tank for ventilated culture, load 70% of the medium and peanut oil defoamer according to the volume, sterilize at 116°C for 40 minutes, and then inoculate the secondary seed liquid according to 5% of the total amount of the medium to gradually increase Large ventilation method, culture at 37°C for 22 hours, type I and type II respectively;

[0041] (2) Pure inspection: After the bacterial culture is completed, take samples for pure inspection;

[0042] (3) Viable bacteria count: Measure the OD525 value, dilute the bacterial solution to an OD525 value of 1, and multiply the dilution factor by 5×10 9 CFU counts viable bacteria, and the counting results ...

Embodiment 3

[0048] Embodiment 3 Ribella anatipestifer inactivated vaccine effectiveness test

[0049](1) Antivirus protection test

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Abstract

The invention relates to a Riemerella anatipestifer bivalent inactivated vaccine and a preparation method thereof, which belongs to the technical field of preparing livestock biological preparations and controlling poultry diseases. The antigens of the vaccine are a Riemerella anatipestifer serum I strain and a serum II strain, and the proportion of the strain antigens is proper in fit. The immune period can be as long as more than 70 days through one injection of the vaccine, and the immune protection efficiency to serum I and serum II reaches more than 90 percent. The vaccine has the advantages of simplifying immunization procedures, reducing the stress reaction of inoculate animals and saving cost for production and injection. The vaccine used for commercial production can end the reality that the control on duck infectious serositis in China excessively depends on drugs, and can provide strong technical support and material guarantee for the healthy stable development of the duck breeding industry.

Description

technical field [0001] The invention relates to a bivalent inactivated vaccine of R. anatipestifer and a preparation method thereof, belonging to the technical field of preparation of veterinary biological preparations and poultry disease prevention and control. Background technique [0002] Riemerellaan atipestifer (RA) disease is currently one of the most serious infectious diseases to the duck industry. It often causes fibrinous pericarditis, perihepatitis, air sac inflammation and caseous salpingitis in ducklings clinically. , conjunctivitis, arthritis, etc., the morbidity and mortality are high. The pathogenic bacteria of the disease is R. anatipestifer. Some strains of this bacteria are sensitive to some antibacterial drugs, but it is difficult to remove them, especially in duck plague. After Bacillus invades the air sac, it is difficult for the drug to reach. At present, due to unreasonable drug regimens, the phenomenon of drug resistance of R. anatipestifer is wides...

Claims

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Application Information

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IPC IPC(8): A61K39/116A61P31/04
Inventor 赵亚荣陈义锋赵建增张渊魁
Owner INST OF ANIMAL HUSBANDRY & VETERINARY FUJIAN ACADEMY OF AGRI SCI
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