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Biological weeding organic fertilizer, and preparation method and application thereof

An organic fertilizer and biological technology, applied in the field of biological weeding organic fertilizer and its preparation, can solve problems such as pollution, hazards, and water quality degradation, and achieve the effects of reducing environmental pollution, reducing costs, and reducing pollution

Active Publication Date: 2016-02-17
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Chemical herbicides have been used to effectively control many weeds in the past century, but the extensive use of chemical agents has also caused a series of problems, such as the emergence of herbicide-resistant weed plants, soil pollution, water quality degradation, and the The harm of weed organisms (especially humans and animals), etc.

Method used

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  • Biological weeding organic fertilizer, and preparation method and application thereof
  • Biological weeding organic fertilizer, and preparation method and application thereof
  • Biological weeding organic fertilizer, and preparation method and application thereof

Examples

Experimental program
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Effect test

preparation example Construction

[0036] (1) Preparation of Sclerotinia rosenbergii SC64 mycelium: inoculate Sclerotinia rosenbergii strain SC64 in sterilized PDA solid medium, activate at 28°C for 5 days, and inoculate the above-mentioned Put 10 pieces of activated Sclerotinia rosenbergii SC64 bacterium cakes in potato liquid medium (potato liquid medium is prepared by the following method: 200g fresh peeled diced potato juice, 20g sucrose, add distilled water to 1L, after configuration Adjust its pH value to 5.0, conventional high-pressure steam sterilization (25min), culture at 30°C, 110rpm for 5 days, centrifuge at 9000rpm for 18min in a high-speed centrifuge, pour the supernatant, and obtain mycelium. Quality as the benchmark, the effective concentration of Sclerotinia rosenbergii Sclerotiorum SC64 in mycelia is 2.86×10 5 cfu / g.

[0037] (2) Raw material crushing: garden waste or crop straw is used as a solid matrix, and it is crushed into fine powder by a straw crusher, passed through a 20-mesh sieve, p...

Embodiment 1

[0040] Compounding of embodiment 1 garden waste and organic nitrogen fertilizer

[0041] Take the crushed garden waste and organic nitrogen fertilizer (soybean cake fertilizer), mix the garden waste and organic nitrogen fertilizer in different proportions, then add glutinous rice flour and water to mix, make granules in a granulator, and dry in an oven Afterwards, put it in an autoclave for sterilization (121°C, 25min), and use it as the base grain for later use. The mass percentage of organic nitrogen fertilizer (soybean cake fertilizer) in the base grain is shown in Table 1, and the percentage of glutinous rice flour in the base grain The mass percentage content is 15%, and the mass percentage content of water in the basal particles is 10%; then, in the granulator, the mycelia of Sclerotinia rosenbergii SC64 is wrapped in the system according to the mass ratio of 1.8:1. The surface of the formed base particle is prepared to obtain a solid granule with a particle size greater...

Embodiment 2

[0043] The bio-herbicide organic fertilizer containing mycelium prepared in Example 1 was detected. Among them, the pathogenicity is detected by the necrotic lesions caused by herbicide particles with a diameter of 3.8 mm in the center of mature Eupatorium adenophorum leaves (5 to 6 true leaves from the top down) at 30°C for 72 hours. Area is an indicator. The freshly cultured mycelium-containing bio-herbicide organic fertilizer is placed in the center of the Eupatorium adenophorum leaf in a 9cm petri dish covered with moist double-layer filter paper in advance, with 2 leaves per dish, Parafilm (AmericanNationalCan, Greenwich, CT, USA) Sealing is carried out, and the culture dish is placed in a 30°C, 12hL / D light incubator. Each experimental treatment was repeated 4 times. Set the granule-coated bacteria without adding organic nitrogen fertilizer as the blank control (CK). The results are shown in Table 1.

[0044] Table 1 The effect of garden waste and organic nitrogen fe...

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Abstract

The invention discloses a biological weeding organic fertilizer. The biological weeding organic fertilizer is a solid granule prepared through wrapping a base granule with Sclerotium rolfsii Sacc. SC64 mycelia; a mass ratio of the Sclerotium rolfsii Sacc. SC64 mycelia to the base granule s 1.7-2.0:1; and the base granule is prepared from, by mass, 5-45% of an organic fertilizer, 1.3-28.6% of an adhesive, 10-15% of water, and the balance of a solid matrix. The invention also discloses a preparation method of the biological weeding organic fertilizer, and an application of the biological weeding organic fertilizer in control of dicotyledon weeds. The biological weeding organic fertilizer simultaneously has the advantages of a traditional organic fertilizer and a biological herbicide, so garden wastes and crop straws are fully used to realize changing of wastes into valuables and reduce environment pollution caused by straw burning, and the use of chemical fertilizers and pesticides is reduced to enhance the stress resistance of crops and improve the quality of the crops.

Description

technical field [0001] The invention belongs to the field of herbicide preparations, and relates to a biological herbicide organic fertilizer and its preparation method and application. Background technique [0002] In recent years, with the large-scale use of chemical fertilizers, the effect of increasing agricultural production is obvious. However, the extensive use of chemical fertilizers has also caused environmental pollution problems such as the increase of heavy metals and toxic elements in the soil, the reduction of microbial activity, nutrient imbalance, and increased acidification. Under the background that the world calls for the sustainable development of agriculture, organic agriculture came into being. Studies have shown that organic fertilizers are not only nutritionally balanced and comprehensive, but also activate potential nutrients in the soil, greatly improving soil biodiversity and soil biological activity, improving soil physical and chemical propertie...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/00A01G1/00A01G13/00
Inventor 强胜张雅丽李俊平李玲燕
Owner NANJING AGRICULTURAL UNIVERSITY
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