245 results about "Dicotyledon" patented technology

The present invention is related to a process based on a source-sink principle, for producing products of interest from crushed or uncrushed germinating dicot seeds comprising an expression system, which is induced or can be induced during germination. The product is either a seed derived composition comprising one or more gene products. Alternatively, it is a product of interest obtained by placing the composition in contact with a substrate, containing a substance capable of being transformed by the seed derived composition as such, dried or in down-stream processed form.

The invention relates to application of Arabidopsis transcription factor in breeding drought-resistant salt-tolerant rice. Nucleotide sequences of the Arabidopsis transcription factor MYB44 are shown in SEQ ID NO.1. Encoded protein sequences of the Arabidopsis transcription factor MYB44 are shown in SEQ ID NO.2. Conditions that are met for the nucleotide sequences and the encoded protein sequences include: first, DNA (deoxyribonucleic acid) sequences shown on the 88th site to the 1005th site in the sequence table SEQ ID NO.1, or the sequences highly homologous to the DNA sequences shown on the 88th site to the 1005th site in the SEQ ID NO.1; second, other encodable sequencecs with the same protein as the DNA sequences shown in the sequence table SEQ ID NO.2; third, the sequences the same functional as the DNA sequences shown on the 88th site to the 1005th site in the SEQ ID NO.1, or sub-segments contained in the highly homologous DNA sequences shown on the 88th site to the 1005th site in the SEQ ID NO.1. The Arabidopsis transcription factor is applied to breeding of the drought-resistant salt-tolerant rice. An expression vector of the MYB44 gene can be introduced into plant cells by biotechnology. Transforming hosts, available to use the expression vector containing the MYB44 gene, can be monocotyledons such as rice, corn and wheat. The Arabidopsis transcription factor is also applicable to dicotyledons such as tobacco and soybean. The Arabidopsis transcription factor is used to breed drought-resistant salt-tolerant plant varieties.

The invention discloses a special expressing promoter of plant endosperm and appliance, which is characterized by the following: 1) possessing DNA sequence of sequence 1 in sequence table; 2) possessing 70% or above 70% homologous property with limited DNA sequence of sequence 1 in sequence table; possessing DNA sequence with same function; 3) possessing crossing nucleic acid sequence with limited NDA sequence of sequence 1 in sequence table under high strict condition. This promoter can start special expression of external source gene in plant endosperm, which is fit for any plant with endosperm such as monocotyledon or dicotyledon.

The invention relates to an alfalfa stress response gene MsNAC3 and an application thereof. A novel NAC (N-Acetyl Cysteine) family related gene is cloned and authenticated from an alfalfa genome by using an RT-PCR (Reverse Transcription-Polymerase Chain Reaction) technology and is named as MsNAC3 with a nucleotide sequence as shown in SEQ ID No.11, and a fusion expression vector containing an enhanced green fluorescent protein (EGFP) gene is constructed. The expression pattern of the gene MsNAC3 in alfalfa and the relation between the gene and adversity stress (cold damage, salt damage and drought) are analyzed by using a fluorescent quantitative PCR technology, in such a way, people find that the cold resistance, drought resistance and salt resistance of a transgenic tobacco plant can be improved through the overexpression of the gene in tobacco; the gene can be used for genetically transforming other monocotyledons and dicotyledons and improving the stress resistance of the monocotyledons and the dicotyledons.

The disclosure describes a transgenic dicot or monocot plant having bovine milk protein(s) and methods of producing the transgenic dicot or monocot plant containing bovine milk protein(s). These transgenic dicot or monocot plants can express and produce bovine milk protein(s). The methods involve introducing a recombinant DNA construct expressing a bovine milk protein into a dicot or monocot plant, obtaining the dicot or monocot plant containing the bovine milk protein(s) from a recombinant DNA construct, cultivating and harvesting the transgenic dicot or monocot plant, and extracting and purifying the bovine milk protein(s) from transgenic dicot or monocotyledonous plants.

The invention discloses a soybean zinc finger protein gene SCTF-1 which is a C2H2-type zinc finger protein gene which is discovered in soybean and is relevant with low temperature. The experiment shows that the protein coded by SCTF-1 can be located to a cell nucleus; after being excessively expressed, the gene can obviously improve cold resistance of transgenic plants, and has a function of improving plant comprehensive stress tolerance. The gene provided by the invention is derived from soybean, and comprises an excellent codon of dicotyledon, such as soybean and the like; and the gene engineering receptor of the gene is mainly suitable for dicotyledon, such as soybean, tobacco and cotton and the like, and also suitable for monocotyledon, such as rice, wheat and corn and the like.

The invention discloses a method for cultivating anti-cyanosis transgenic cotton and a special expression vector thereof. The method for cultivating the anti-cyanosis transgenic cotton comprises the following steps: introducing the protein-coding genes of an SPGAFP1 protein into a target plant to obtain a transgenic plant with the cyanosis resistance higher than that of the target plant, wherein the amino acid sequence of the SPGAFP1 protein is sequence 2 in a sequence table; the sequence of the protein-coding genes of the SPGAFP1 protein can be sequence 1 in the sequence table; and the target plant and the transgenic plant are dicotyledon, i.e. cotton, preferably the CCRI 24. Experiments prove that the SPGAFP1 cotton obtained by introducing the expression vector into the cotton can effectively resist fungi initiating cyanosis, thus indicating that the existence of signal peptide greatly enhances the disease resistance of the transgenic plant.

The invention belongs to the technical field of biology, and particularly discloses a gene editing vector system based on barley stripe mosaic virus. The gene editing vector system comprises artificial plasmids respectively containing RNA[alpha], RNA[beta] and RNA[gamma] of the barley stripe mosaic viruses; and in the RNA[beta] or the RNA[gamma], a desired sgRNA sequence is integrated. The gene editing vector system based on the barley stripe mosaic virus can perform efficient gene editing on genomes of dicotyledons such as nicotiana benthamiana and monocotyledons such as wheat and corn.

The invention provides a bionic microfluidic cell culture chip. The bionic microfluidic cell culture chip is based on the moisture conveying characteristic in a dicotyledon reticular vein, and comprises a main channel (6) and a cell culture cavity (9) located in the main channel. The cell culture cavity (9) is isolated from the main channel through a micro-column array (8) with the shape consistent with the shape of the culture cavity. The width of slits of the micro-column array (8) is determined by the size of cultured cells. Cell culture fluid is conveyed to the cell culture cavity through the slits of the micro-column array (8) in a convection diffusion mode, direct impact of the cell culture fluid on the surfaces of cells is avoided, influences of the cell culture fluid on cells are reduced, a stable microenvironment is provided for cell culture in vitro, and cell research accuracy is ensured.

The invention relates to a promoter derived from powdery mildew of rubber trees and application of the promoter. The invention provides a novel promoter derived from powdery mildew of rubber trees, and the promoter has a nucleotide sequence as shown in SEQ ID NO:1, or a variant with functions of the promoter. The invention further relates to a nucleic acid structure, a vector, a recombinant cell,a transgenic plant, explants and calluses with the promoter. The invention further relates to application of the promoter to regulation and control of target gene expression in fungi. The promoter canbe used for regulating and controlling expression of exogenous target genes in dicotyledon and monocotyledon, and a completely novel tool and option are provided for gene expression of transgenic plants.