Efficient cleft grafting method of dicotyledon seedlings and young branches

Abstract

The invention discloses an efficient cleft grafting method of dicotyledon seedlings and young branches. Rootstocks with 1-2 true leaves (only 2 cotyledons are reserved) or rootstocks with 4-6 true leaves (2 cotyledons and 1-2 true leaves are reserved) are adopted, and stems and the lower edges of petioles are vertically split in the middle along the central axes of the stems from the lower edges of the petioles of the cotyledons and the true leaves; the incisions of scions are cut into a V shape; the stems of the scions are perpendicularly inserted into the incisions of the rootstocks. The incisions of the rootstocks run through the lower sections of the petioles and the central axes of the stems at the same time, so that fixation of the scions is convenient, the scions are closely combined with the rootstocks naturally, nutrient substances produced by photosynthesis of the leaves are transported into the scions nearby through the petioles, and rapid healing of connectors is promoted. In combination with water retention and heat preservation in a recovery period, the survival rate of grafting can be stabilized at 98% or above, and the survival rate of grafting can reach 100% in an appropriate cooperation period of the rootstocks and the scions; according to the method, operation is easy and convenient, grafting is fast, and the survival rate is high.

Description

technical field [0001] The invention relates to a splitting method for plant seedlings and young shoots, in particular to a high-efficiency splitting method for dicotyledonous plant seedlings and young shoots, and belongs to the field of agricultural technology. Background technique [0002] Genetic engineering and cell engineering are important methods for genetic improvement of cotton today. Cotton tissue culture regeneration plants are the basis for the application of transgenic technology in cotton genetic improvement. However, most of the test-tube seedlings of transgenic regeneration cotton plants obtained through somatic embryogenesis have stunted root systems. It manifests as rootless seedlings, brown rooted seedlings, and deformed seedlings (such as clustered seedlings), etc., and the regenerated plants with normal root systems are transplanted into soil or vermiculite, the survival rate is extremely low (usually only 10-20%), and the growth is slow, which greatly li...

AI Technical Summary

Problems solved by technology

[0006] (1) The conventional "split grafting method" sticks to the cotyledon petiole to remove the head and remove the heart, the incision is perpendicular to the cotyledon petiole, the cotyledon will droop to both sides due to gravity, and it is difficult to insert and fix the scion

There are also some researchers who leave a section of epicotyl at the upper end of the cotyledon petiole and split it longitudinally, but this method is not convenient for rootstock blades to supply scion nutrition;

[0007] (2) The rootstock required by the "skin grafting method" is relatively large;

[0009] Therefore, the grafting technology of cotton still needs in-depth and systematic research, and a perfect cotton grafting technology system has yet to be established.

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