174 results about "Phyllopodium" patented technology

The invention aims to provide a grafting method with minimum cut area for improving grafting survival rate, in particular a method for grafting different plants on the same plant at high survival rate and with simpleness, feasibility and wide application range. The grafting method comprises the following steps: (1) cion and parental stock selection, during which, an individual plant without disease and pest is selected as a parental stock and a wattle with the diameter similar to that of the parental stock is selected as a scion; (2) cion cutting, during which, 3 to 6 minutes of the scion is cut as a cion with one bud and one leaf (leafstalk only) left and the section perpendicular to the longitudinal direction of the wattle taken as a grafting surface; (3) parental stock cutting, during which, the parental stock is cut at the grafting height with the section perpendicular to the height direction of the parental stock taken as a grafting surface; (4) cion and parental stock combination, during which, the cion section and the parental stock section are tightly attached to align a cambium; and (5) interface binding and fixing, during which, an interface is fixed preliminarily by an adhesive tape and then the cion is tightly wrapped by a plastic film strip or a small-opening plastic bag so as to be fixed on the parental stock.

The invention discloses a method for establishing a lotus regeneration system. The method comprises the following steps: breaking shells of sterilized Guangchang white lotus seeds by using pruning shears; taking out mature lotus nuts (embryos) as explants to culture aseptic seedlings; cutting 2-3mm of stem tips and folded tender leaves (with short petiole) as explants for inducing callus from the aseptic seedlings after 2 months; inducing and differentiating the callus to develop buds after 2 months; after 1 month, cutting off the black callus at the bottom, and proliferating the differentiated seedlings; after 3 months, inducing the differentiated seedlings to develop roots; and strengthening, domesticating and transplanting the rooted tissue culture seedlings after 1 month. By adopting the method, technical basis is provided for transgenic technology of lotus in China, and the inductive callus rate of lotus is increased and the callus is large and stable in size compared with a conventional technique; the propagation coefficients of adventitious buds are increased; as tender leaves of aseptic seedlings of lotus are innovatively taken as explants for inductive callus and are successfully differentiated to obtain seedlings, the material selection of lotus inductive callus is widened, and material guarantee is provided.

The invention discloses a method for culturing golden larch callus by taking phyllopodium as an explant. The method comprises the following steps of: disinfecting buds of the golden larch; cutting the phyllopodium on the buds after being disinfected and taking the phyllopodium as the explant; culturing by implanting the phyllopodium into a callus induced culture medium: MS+6-BA 1.0-2.0mg / L+NAA 0.1-1.0 mg / L+IAA 0.1-0.5 mg / L+ cane sugar 20-50 g / L+ agar 5.0-7.0 g / L and a multiplication culture medium: MS+6-BA 0.5-1.0mg / L+NAA 0.1-1.0 mg / L+ cane sugar 20-50 g / L+ agar 5.0-7.0 g / L or MS+6-BA 0.5-1.0mg / L+IAA 0.1-1.0 mg / L+ cane sugar 20-50 g / L+ agar 5.0-7.0 g / L; and finally realizing the effective induction and quick multiplication of the golden larch callus. According to the method provided by the invention, the present problem of being short of golden larch medicinal materials is effectively solved.

The invention relates to a method for simply and efficiently producing pinellia ternate embryoid and tissue culture seedling. The method is characterized in that an MS (Murashige and Skoog) solid culture medium is used in a production process, lingfasu(LFS) with the concentration of 0.3mg / L (the concentration is effective in the range from 0.05 to 2.0) is added into the MS solid culture medium to be prepared into a 0.3mg / L MS+LFS single culture medium, pinellia ternate petiole slices (or blades or other separated organs) are taken as a successive propagation material, and all the related technical links such as 'callus induction', 'embryoid induction', 'mature seedling induction', 'multiplication and propagation', 'rooting' and the like can be completed in one step by using the single culture medium. The embryoid inductivity reaches 80-100%; one successive cycle comprises 30 days, multiplication times of the tissue culture seedling can reach 10-20 or higher. By applying the method, the production process of pinellia embryoid and tissue culture seedling is simplified, and the production efficiency is improved.

The invention discloses an in vitro tissue cultivation method of potted anthurium andraeanum varieties. A leaf blade, a leaf stem, a flower head, a spathe blade or a lateral bud is taken as an explant material; after the explant material is sterilized, the explant material is inoculated to an appropriate culture medium and cultivated for 40-60 days, and then dedifferentiates to form a callus, and the callus rate can reach 87.5%; the material is continuously cultivated for 40-60 days and differentiates again to form an adventive bud, and the differentiation rate can reach 94.12%; and a large quantity of tube seedlings can be rapidly cultivated through multiplication, when the tube seedlings grow to certain heights, the tube seedlings are transferred into the culture medium and transplanted after the tube seedlings take root, and the survival rate can be larger than 95%. With the adoption of an in vitro tissue cultivation technology, a regeneration plant can be stably and efficiently obtained through the way that the callus induces the adventive bud, and rapid and mass propagation of the potted anthurium andraeanum varieties can be achieved; and according to the characteristics of different anthurium andraeanum varieties, the appropriate explant material and a culture medium condition are selected, and the method has a high application value in the production of seedlings of excellent anthurium andraeanum varieties.

The invention discloses a breeding method of a heat-resistant red-skin radish and belongs to the technical field of breeding of brassicaceous vegetables. The breeding method is technologically characterized in that the first generation of hybrid of heat-resistant red-skin white radish is obtained by using a sterile line of red-skin radish as a female parent and a red high-generation inbred line of a local variety red-skin white radish in Sichuan province as a male parent. The first generation of hybrid of heat-resistant red-skin white radish has the characteristics that the plant leaves are semi-erect and are plate leaves, blades are green, leaf stalks and middle stems are red, the leaf surface has seta, the leaves are inverted oval-shaped, the leaf margins are crenate, the leaf length is about 36cm, the fleshy root is of a long cylinder shape, the skin is smooth and red, the flesh is white, the root length of the product is 20-25cm, the transverse diameter is 5-6cm, and the single weight is about 450g. The heat-resistant red-skin radish is strong in disease resistance and heat resistance, good in commodity property, excellent in quality, not easy to be hollow, short in growth period, and suitable for outdoor cropping in various regions in summer, autumn and winter.

The invention relates to a rooting method of subprostrate sophora root tissue culture seedling leaf stalks. The method comprises the following steps that: (1) subprostrate sophora root tissue culture seedlings are inoculated into a Murashige and Skoog (MS) seedling strengthening culture medium for strengthening the seedling so that tissue culture seedling leaves are spread to obtain the complete high-lignification-degree compound leaves with leaf stalks; and (2) the compound leaves with leaf stalks are placed into an MS rooting culture medium to be cultured and inducted for rooting. When the method provided by the invention is adopted, a large number of root materials are obtained from the subprostrate sophora root tissue culture seedling leaf stalks, and a novel path is provided for subprostrate sophora root medical material resources and active ingredient extraction material sources.

The invention provides a bud grafting method of a precocity thin-skin walnut. The bud grafting method of the precocity thin-skin walnut comprises the following steps of (1) selecting a cut stem of a walnut seedling tree, and culturing 1-2 strong buds in the different directions for standby application after germination; (2) carrying out scion collection and preservation, wherein a developmental branch of the precocity thin-skin walnut is collected to serve as a scion in June or July of the current year, compound leaves and shoots need to be timely removed after the scion is picked, and a 1-2cm petiole is retained; (3) forming a grafting opening, wherein a rootstock notch is formed in the straight smooth portion of the lower half portion of a rootstock branch; (4) obtaining a bud tuber, wherein a bud tuber notch is formed in a full bud which is arranged on the lower half portion of the scion; (5) carrying out grafting, wherein the scion is embedded into the rootstock notch, and the bud tuber notch can be closely connected with the rootstock notch; (6) carrying out sealing on a connector, wherein a plastic strip is used for being tightly wound on the grafting portion from bottom to top, and the buds are exposed. The bud grafting method of the precocity thin-skin walnut can greatly short the breeding cycle, and improves correctness of variety breeding and breeding efficiency.

The invention discloses a method for breeding michelia variety Yunrui. The method comprises the following steps of: hybridizing michelia yunnanensis serving as a female parent and michelia doltsopa serving as a male parent to obtain a filial generation; and after the filial generation flowers, hybridizing the filial generation serving as a female parent and michelia maudiae serving as a male parent to obtain the michelia variety Yunrui. The new variety 'Yunrui' integrates partial excellent traits of three parents: the shrub is branched, dense and glabrous throughout. The leaves are leathery and oblong to inversely oval long circle, the length is 16 to 18 centimeters, the width is 6 to 8 centimeters, the tip is acute, and the base is narrow and wedge-shaped; the leaves are deep green and glossy; and the petiole nearly has no stipule scars. The flowers are milky white and fragrant; the number of tepals is 9 to 10, the tepals are inversely oval, the length is 6 to 7 centimeters, the width is 3 to 4 centimeters, the base is light yellow, and the tip is short and small; the carpel is green, the style is purple red, the androeceum is light yellow, and the stamen of the base is petalled; and the flowers are expanded and broadly campanulate.

The invention discloses a method of breeding hills-of-snow Forever Summer quickly by using leaves of a tissue culture seedling. The method comprises the following steps: A) scissoring the third to sixth acrogenous leaves of the sterile tissue culture seedling of Forever Summer and cutting the leaves vertical to the main vein and removing petiole and the tip; B) putting the blades onto a callus induction culture medium for illuminating culture after dark culture; C) after illuminating culture, transferring the blades with callus to a callus proliferation and adventitious bud induction culture medium for subculture to form multiple shoots; D) slitting the multiple shoots, and transferring the multiple shoots to an adventitious bud growth culture medium for elongation growth to form a seedling; and E), cutting the seeding down, and transferring the seedling to a seedling growing and rooting culture medium, wherein the seedling develops an intact regenerated plant with roots, stem and leaves. By adopting the Forever Summer sterile tissue culture seedling leaves to induce callus and differentiating the adventitious bud by the callus, the problems that an explant needs to be sterilized,the pollution is high and the like in the Forever Summer tissue culture process are solved.

The invention discloses a monkshood-tuber tissue culture and rapid propagation method, which comprises the main steps of selection, disinfection and sterilization of an explant, inoculation and induction of callus, induction of cluster buds, root induction, hardening-seedling and transplanting and the like. By carrying out tissue culturing on stem tips, blades, roots, stem sections, leafstalks, seeds and the like of monkshood-tuber as the explants, and inducing a plant to regenerate and rapidly propagate monkshood-tuber seedlings, the inductivity, the differentiation rate and the rooting rate are higher, and the survival rate of the seedlings after being transplanted in culture soil is high. The method can effectively overcome the problems of poor disease resistance, low and unstable output, varietal complexity and the like of a traditional monkshood variety in the production of the monkshood-tuber, realizes the variety purification and rejuvenation through stem tip detoxification, tissue culture and other means, and provides excellent monkshood varieties, and lays a favorable foundation for the factory and scale production of large quantities of high-quality seedlings required by monkshood GAP (Good Agricultural Practices) bases and the like.

The invention discloses a method of fast breeding peony, comprising: a) getting axillary bud bulk tip or tender leaf stalk from plants and culturing them in the culture medium until the callus formation and germination, b) cutting off the clump bud from the callus and culturing them until the bud grows to a height of 3-5 cm; c) inserting the bud in step b) into solutions containing indolebutyric acid, then culturing in the MS solid culture medium added with indolebutyric acid, benzyl amino adenine, active carbon, saccharose and macronutrient whose concentration reduced halves to form small plants; d) replanting the small plants. The invention develops a method of mass fast breeding peony by employing technique of plant cell tissue and forms a mass production process which produces tube sprout of peony ten thousand plants per year.

The invention provides a Chuanminshen violaceum tissue culture method. According to the method, the stem tip, tender leaves and fresh tender leaf stalks of the Chuanminshen violaceum plant with good characters in the vigorous growth period are utilized as explants, and the method comprises the following steps of: performing disinfection treatment and inoculation induction to form callus; performing callus multiplication, bud induction culture and rooting culture to obtain complete Chuanminshen violaceum plant seedling; and transplanting the complete seedling into a seedling culture medium, and culturing to obtain a Chuanminshen violaceum annual seedling. All the culture media are based on an MS culture medium in combination with components such as 6-benzyladenine, naphthylacetic acid, 2,4-dichlorphenoxyacetic acid, indolebutyric acid, sucrose an agar. The method provided by the invention can be used for realizing the tissue rapid propagation of Chuanminshen violaceum by use of a plant tissue culture technology, and has important application values in terms of annual seedling supply and improved-variety rapid expanding propagation of the industrialized cultivation of Chuanminshen violaceum.

The invention discloses a method for breeding a cold-resistant eucalypt Xiang eucalypt No.1 variety suitable for the north latitude 26 to 30 degrees. The method comprises the following steps of: determining Eucalyptus dunnii as a eucalypt variety, sterilizing a culture medium, sterilizing and disinfecting before inoculation, inoculating, subculturing, performing rooting culture, performing seedling culture, and culturing young seedling and sapling, wherein the inoculation is performed by the following steps of: (1) scissoring robust semi-lignified sprout branches, preserving heat with clean gauzes or sealing with clean gauzes for moisturizing, and bringing back to a room at a sunny noon or afternoon; (2) cutting away all leaves, leaving a small number of leaf stalks, soaking in 0.1 percent mercuric chloride solution for 3 to 12 minutes section by section according to the lignification degree of the branches, and washing with sterile water for 4 to 5 times; and (3) cutting stalk sections with sprouts, wherein the stalk sections are 1 to 2 centimeters and the branches of the upper branch sections of lateral buds are about 0.5 centimeters, inoculating on a culture medium of a 3 / 4ms+0.5 milligram / L IBA+0.3milligram / L NAA culture bottle, controlling the temperature to be 22 to 27 DEG C and illumination intensity to be 2,000 lx, and inducing the lateral buds to germinate.

The invention discloses a method for producing pinellia-ternate seed stems, and belongs to the field of traditional Chinese medicinal material planting. The method includes the steps of explant treating, in-vitro corm induction, greenhouse domestication and the like. Petioles are selected as explant for tissue culturing, pollution is low, the induction effect is good, the induction effect of in-vitro corm induction culturing is good, the petioles are subjected to induction culturing for 100 days to 120 days, and in-vitro corms with buds for greenhouse domestication can be obtained. By means of the method for producing the pinellia-ternate seed stems, it is achieved that the in-vitro corms are obtained through direct induction of the explant, the callus induction process, the embryoid induction process, the rooting induction process are reduced, one step is only needed, and the in-vitro corm induction step is greatly simplified. Soilless culturing is adopted in greenhouse domestication, infection of outside germs is reduced, disease is reduced, and the method has the high resistance. The in-vitro corms are sowed on a net with the certain specification, and the harvesting efficiency is improved. A large amount of high-quality pinellia-ternate seed stems can be harvested after domestication culturing is carried out for one season for supplying large-area cultivation of a field, the whole production technology is simple and easy to operate, operating is easy, and the method is largely suitable for larger-scale production.

The invention belongs to the technical field of agriculture and concretely relates to a simplified sweet potato vine seedling nursing method for edible sweet potatoes. In the method, strong sweet potato vines free of frost damage, diseases and mechanical damages are selected; three joints are remained upwardly from the root for each cirrus on the upper part of the sweet potato vine; laminas are removed and one centimeter of petiole is preserved; then the petiole is transplanted to a storage nursery garden for overwintering; after the cirrus on the sweet potato vine sprouts, new sweet potato vines are harvested 7 to 10 days before transplantation to the large field to work as germchits; laminas of the harvested new sweet potato cirrus are removed and one centimeter petiole is left; each two-joint is cut and planted in the ground in a greenhouse or the ground in a three-film double-arched shed and thoroughly watered until the depth of water is 5cm; the upper part of a sweet potato vine section gives out a small bud after 7 to 10days and the lower part germinates a certain root system; and effective germchits are cultured and can be transplanted to a production field. The simplified sweet potato vine seedling nursing method for edible sweet potatoes fully utilizes waste sweet potato vines for seedling nursing, so potato seeds are saved; edible sweet potato seed storage, overwinter and rot problems can be avoided; and sweet potato seedling nursing labor intensity can be reduced.

The invention discloses an efficient cleft grafting method of dicotyledon seedlings and young branches. Rootstocks with 1-2 true leaves (only 2 cotyledons are reserved) or rootstocks with 4-6 true leaves (2 cotyledons and 1-2 true leaves are reserved) are adopted, and stems and the lower edges of petioles are vertically split in the middle along the central axes of the stems from the lower edges of the petioles of the cotyledons and the true leaves; the incisions of scions are cut into a V shape; the stems of the scions are perpendicularly inserted into the incisions of the rootstocks. The incisions of the rootstocks run through the lower sections of the petioles and the central axes of the stems at the same time, so that fixation of the scions is convenient, the scions are closely combined with the rootstocks naturally, nutrient substances produced by photosynthesis of the leaves are transported into the scions nearby through the petioles, and rapid healing of connectors is promoted. In combination with water retention and heat preservation in a recovery period, the survival rate of grafting can be stabilized at 98% or above, and the survival rate of grafting can reach 100% in an appropriate cooperation period of the rootstocks and the scions; according to the method, operation is easy and convenient, grafting is fast, and the survival rate is high.

The invention relates to the technology of plant tissue culture, and in particular relates to a tissue culture method for inducing populus yunnanensis dode tetraploid in combination with colchicine. The method taking petiole of populus yunnanensis dode as a tissue culture material in the process of tissue culture is characterized by comprising the following steps of: adding colchicine into a populus yunnanensis dode petiole differential medium to induce populus yunnanensis dode to generate polyploidy, wherein the treatment time is 20-30 days; carrying out differentiation culture again in a populus yunnanensis dode petiole differential medium in which colchicine is not added after the treatment; cutting the part which is obvious in variation on the differentiated cluster buds; repeatedly cultivating on the differential medium for 5-6 times, so that the populus yunnanensis dode petiole differential medium is stable in polyploidization variation; and performing rooting on the clump seedlings which are stably varied, so that the tissue culture seedlings of the tetraploid populus yunnanensis dode can be obtained. The method provided by the invention is the tissue culture method for inducing the populus yunnanensis dode tetraploid in combination with the colchicine, which is easy and simple to operate and can effectively reduce the chimera.

A production method of a germplasm resource of ornamental flowering Chinese cabbages is characterized by comprising steps as follows: a conventional variety of common cabbages, that is, Suzhongqing Q8, used as a female parent and Guangdong cabbage selfing line G1 used as a male parent are hybridized to form first hybrid generation seeds F1; first hybrid generation seeds F1 have selfing to form F2; single plants with shortest petioles are selected from F2 to have selfing to form F3; single plants with compact plant types, short petioles and small leaves are selected from F3 to have selfing to form F4; single plants with compact plant types, short petioles, small leaves and glossy dark green cabbage color are selected to have selfing to form F5; single plants with compact plant types, short petioles, small leaves, glossy dark green cabbage color and short joints are selected to have selfing to form F6; single plants with compact plant types, short petioles, small leaves, glossy dark green cabbage color, short joints and upright leaves are selected to have selfing to form F7; single plants with lotus-throne-shaped leaf clusters, unobvious base jointing during cabbage drawing, short stems, long flowering period and large flower amount are selected to has selfing to form F8. The ornamental period of the germplasm leaves and flowers of the ornamental flowering Chinese cabbages is 40-60 days, leaves and flowers can be enjoyed during the New Year's Day and the Spring Festival, the flower amount is large, and the ornamental effect is good.

The intention discloses a method for consistent hole tray seedling cutting for photinia fraseri, and belongs to the field of seedling cultivation. The method includes that fresh and vigorous photinia fraseri branches are harvested and subjected to strict grading to guarantee the same to be consistent in size, cuts of seedlings are preferably 3-3.5cm in length, and each seedling reserves one leaf and one bud; an upper cut is located 2mm above an axillary bud of the reserved leaf to ensure vitality of the axillary bud, and a lower cut is selectively arranged 1mm below a leaf stalk or a stipule scar so as to facilitate rooting; roots of the seedlings are treated by being quickly dipped in hormone, and the seedlings are cut into a hole tray full of matrix according to 40cm X 35cm in space between rows, normal roots are watered sufficiently, and management is followed. By strict cutting control, high-quality consistent seedlings are harvested in large-scale cutting cultivation of the photinia fraseri. Special treatment is given to mother branch collecting, the cuts of the seedlings and biological hormone dipping, and the seedlings are subjected to the management after the seedling cutting, so that large-scale cutting propagation of the photinia fraseri is realized, and high-quality products are obtained.

An Apium graveolens L. var dulce celery plant with a hollow petiole suitable for use as a straw for consuming beverages or as a food product where other food products are capable of being stuffed inside the hollow celery tube. The edible, hollow celery is mild in taste and more resistant to rupturing than celeriac and leaf celery with hollow petioles.

The invention discloses a single-leaf cutting propagation method of panicled fameflower roots. The single-leaf cutting propagation method comprises the following steps of: watering soil thoroughly to form muddy soil, and flattening; collecting fresh single leaves of panicled fameflower roots, inserting 1 / 5 to 1 / 3 length of tip end parts of the single leaves into soil, enabling the upper surfaces of the leaves to point upwards and partial lower surfaces to contact with the soil, enabling basic parts of leaf stalks to contact with the soil and expose in air, performing the root cutting at a certain density, keeping the temperature and the humidity, illuminating for 10-14 hours every day and placing the single leaves in darkness for 10-14 hours, and keeping the soil wet; after the single leaves take roots and sprout, and more than three new leaves are sprouted, spraying leaf fertilizer for once; when seedlings grow to 0.05 m high, transplanting, wherein the planting percent is up to 90%, even higher. In the whole process, panicled fameflower root plants obtained through single-leaf cutting can bloom in 3 months. The single-leaf cutting propagation method of panicled fameflower roots is convenient and simple in operation, low in cost and short in propagation period, and can propagate panicled fameflower roots in different seasons.

The present invention provides an in vitro resveratrol-rich callus tissue of Vitis thunbergii Sieb. et Zucc. which is a callus tissue developed from a tissue culture system containing one or more plant growth regulators (PGRs) and cultured from a stem or a petiole tissue explant of a wild type of V. thunbergii or a cultivated plantlet of V. thunbergii. The cultivated plantlet is in turn derived from a shoot of the wild type of V. thunbergii cultivated in a plantlet culture system containing no PGRs. The in vitro resveratrol-rich callus tissue of V. thunbergii is characterized by its containing at least about 1,000 to 10,000 mg / kg of dried weight of resveratrol, predominantly in the form of trans-resveratrol and / or resveratrol-O-glucoside, and being ready for harvest or subculture in about 30 days. The present invention further provides a method for cultivating the in vitro resveratrol-rich callus tissue, a method for extracting the resveratrol from the in vitro resveratrol-rich callus tissue, and a method for determining the resveratrol amount in the in vitro resveratrol-rich callus tissue by HPLC.

The invention discloses organic fertilizer capable of promoting potato growth. The organic fertilizer is prepared from raw materials in parts by weight as follows: 17-20 parts of sweet potato straw, 18-20 parts of carrot slices, 18-20 parts of boiled soybeans, 17-20 parts of rape seed cakes, 16-20 parts of cottonseed cakes, 10-12 parts of plant ash, 4-6 parts of chicken manure, 3-5 parts of sheep manure, 2-3 parts of rabbit manure, 4-6 parts of cow dung, 5-7 parts of earthworm soil, 1-2 parts of an EM agent and 2-5 parts of a deodorant. The organic fertilizer can improve the biochemical activity of soil effectively, increase the effective fertilizer absorptivity, reduce the soil hardening degree and reduce environmental pollution of the fertilizer. The organic fertilizer can enable potatoes to have developed root systems, strong petioles, large leaves with deep color and thick and large tuberous stems, so that the single yield of the potatoes is increased greatly, and various nutrient elements needed by a human body are increased in the potatoes.

The invention relates to a natural-hybrid seed production method for triploid salvia which takes the purple genes as marker traits; the method takes tetraploid white salvia as the female parent and diploid purple salvia as the male parent; in the field, the male parent and the female parent are planted according to a proportion of 1 to 1; pollination is done by wild hornet and domesticated bees; 2 / 3 of the seeds produced on the tetraploid white salvia female parent plant are triploid salvia, while the remaining 1 / 3 are tetraploid white salvia; the hybrids with purple flowers are triploid salvia, while the hybrids with white flowers are tetraploid salvia; the triploid salvia is slightly purple at stems, leaves and petioles at seedling stage; the invention does not need manual castration, thereby reducing the labor intensity in seed production, saving the labor and decreasing the production cost; besides, the parental seeds can be accurately separated from the hybrid seeds, and the produced seeds have high purity. The invention can be applied to larger-area hybrid seed production and is easy to promote.

The invention provides a method for water culture of zamioculcas zamiifolia. The method is to shear a leafstalk from a mature zamioculcas zamiifolia and directly insert the leafstalk into water for culture to ensure that the leafstalk takes root to obtain a small plant of the zamioculcas zamiifolia; and the method comprises the steps of material selection, pre-cure, water culture and storage. Compared with the prior art, the method has the advantages that the method is clean and sanitary, and has simple and convenient operation, and the small plant of the zamioculcas zamiifolia has long storage time under an anhydrous state.

The invention relates to a transplanting method for osmanthus trees, and relates to the technical field of transplanting of greening tree species. The method is characterized in that the method comprises the following steps: selecting the transplanting season before winter, wherein the air temperature is from 5 to 15 DGE C; digging tree pits: the tree pits in a new transplanting land are dug well about one week before transplanting; trimming: the leaves of the osmanthus are trimmed, the leaves are trimmed by a pair of scissors, leaf stalks are left, auxiliary buds are protected, and the number of the remained leaves is one third of that of the original leaves; lifting seedlings: the osmanthus trees to be transplanted are watered thoroughly before one week, so that the roots can absorb enough water; transplanting: before the transplanting, the previously dug tree pits are backfilled with the original soil transported with the osmanthus trees to 10 to 15 cm; and subsequently managing. The method has the advantages of moisture absorption and water retention. Besides, fertilizers can be increased to the osmanthus trees, the growth of plants is promoted, the survival rate can reach over 85%, and saving water resources and labor cost is facilitated.

The invention belongs to the technical field of flower culture, in particular to a cutting propagation method of peperomia magnolifolia. The method comprises the following steps of: taking matured thriving peperomia magnolifolia blades as cuttings, adopting entire leaf cutting and inserting an inserted leaf with a leaf stem of 0.7-1.4 cm to one third position of each blade; inserting the blades in 72 hollow aperture trays, and cutting 3 blades in each hole, wherein a cutting substrate is a mixture of river sand and turfy soil in a volume ratio of 1:3 to 1:4 as a cutting substrate; spraying the cutting substrate with water, keeping the temperature of 20-25 DEG C and controlling the air moisture to be 85-95 percent during cutting; only watering with prepared water of a pH value of 6.8-7.2 without pouring fertilizer, keeping the temperature of 20-25 DEG C and controlling the air moisture to be 85-95 percent within half a month of cutting propagation.

The invention discloses a method for acquiring transgene cottons. The method comprises the following steps of: (1) cutting cotyledons, leaf stalks and a part of hypocotyl of cotton cotyledon seedlings and keeping r 1-3 cm of the hypocoty to obtain tissues to be infected at 25-30 DEG C and with the relative humidity 40-60%, and culturing for 2-3 days under dark environment; (2) scratching double surfaces from the top of a stem tip to the base part of the hypocotyl with the cut of 0.8-1.2 mm in depth; (3) dip-dyeing for 15-45 min in solution A which contains a recombination agrobacterium carrying exogenous genes; (4) co-culturing for 2-4 days and then carrying out resistance screening to obtain the resistant cotton seedling; and (5) grafting the resistant cotton seedling on a cotton rootstock to obtain the transgene cotton. The invention improves the conversion efficiency through adjusting the growth states of the stem tip, determining the appropriate depth of the agrobacterium-converted stem tip infection, adding acetosyringone and adjusting the proper pH value. Transgene plants can be quickly obtained by using the method, and the higher transgene efficiency is higher.