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Method of breeding hills-of-snow Forever Summer quickly by using leaves of tissue culture seedling

A technology of tissue culture seedlings and leaves, applied in the field of agricultural biology, can solve the problem of low callus induction rate, achieve the effect of improving reproduction coefficient, great scientific research value and economic value, and shortening the reproduction cycle

Active Publication Date: 2019-03-19
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to the above literature reports on inducing callus from hydrangea leaves, the inventor found through repeated experiments that the culture medium and culture conditions reported above are not suitable for Hydrangea grandiflora 'Endless Summer', and the leaves of tissue cultured seedlings of 'Endless Summer' were grown under the above conditions. lower callus induction rate

Method used

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  • Method of breeding hills-of-snow Forever Summer quickly by using leaves of tissue culture seedling
  • Method of breeding hills-of-snow Forever Summer quickly by using leaves of tissue culture seedling
  • Method of breeding hills-of-snow Forever Summer quickly by using leaves of tissue culture seedling

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Experimental program
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Effect test

Embodiment 1

[0040] 1. Obtaining and multiplying sterile tissue culture seedlings of "Endless Summer"

[0041] Cut the 5-7cm-long new branches of "Endless Summer" (with 4-6 side buds), wash them with washing powder, and rinse them with running water for 2 to 3 hours. Under aseptic conditions, first soak in 70% alcohol for 30 seconds, then use 0.1% HgCl 2 Sterilize for 10 minutes, rinse with sterile water 4-6 times, and inoculate it on MS solid medium. After 30 days, cut the obtained sterile seedlings into 2cm (with 2 lateral buds) stems and transfer them to MS+6 -BA 2.25 mg / L+IBA 0.1mg / L medium for proliferation, 30 days later, transfer the proliferated buds to MS+6-BA 1.0mg / L+IBA 0.1mg / L medium for vigorous seedling .

[0042] 2. The effect of leaf position on leaf callus induction

[0043] Cut the 3rd and 4th, 5th and 6th, 7th and 8th, 9th and 10th leaves of the sterile tissue cultured seedlings in step 1 of the "Wan Jin Xia" aseptic tissue culture seedlings, cut a knife perpendicular to the ...

Embodiment 2

[0075] 1. Obtaining and multiplication of aseptic tissue culture seedlings of three large-flowered hydrangea varieties: "Endless Summer", "Shy Ye" and "Ivy Tower"

[0076] Cut out the 5~7cm-long new branches of the year in "Endless Summer", "Shy Leaves" and "Ivy Tower" (with 4-6 side buds), and rinse them with washing powder and water for 2 to 3 hours. Under aseptic conditions, first soak in 70% alcohol for 30 seconds, then use 0.1% HgCl 2 Sterilize for 10 minutes, rinse with sterile water 4-6 times, and inoculate it on MS solid medium. After 30 days, cut the obtained sterile seedlings into stems of about 2cm (with 2 lateral buds) and transfer them to MS+6 -BA 2.25mg / L+IBA 0.1mg / L medium for growth, 30 days later, transfer the proliferated buds to MS+6-BA 1.0mg / L+IBA 0.1mg / L medium for vigorous seedling . The stem proliferation medium MS+6-BA 2.25mg / L+IBA 0.1mg / L and the strong seedling medium MS+6-BA 1.0mg / L+IBA 0.1mg / L are for the three varieties of hydrangea' endless The ste...

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Abstract

The invention discloses a method of breeding hills-of-snow Forever Summer quickly by using leaves of a tissue culture seedling. The method comprises the following steps: A) scissoring the third to sixth acrogenous leaves of the sterile tissue culture seedling of Forever Summer and cutting the leaves vertical to the main vein and removing petiole and the tip; B) putting the blades onto a callus induction culture medium for illuminating culture after dark culture; C) after illuminating culture, transferring the blades with callus to a callus proliferation and adventitious bud induction culture medium for subculture to form multiple shoots; D) slitting the multiple shoots, and transferring the multiple shoots to an adventitious bud growth culture medium for elongation growth to form a seedling; and E), cutting the seeding down, and transferring the seedling to a seedling growing and rooting culture medium, wherein the seedling develops an intact regenerated plant with roots, stem and leaves. By adopting the Forever Summer sterile tissue culture seedling leaves to induce callus and differentiating the adventitious bud by the callus, the problems that an explant needs to be sterilized,the pollution is high and the like in the Forever Summer tissue culture process are solved.

Description

Technical field [0001] The invention relates to a method for rapidly propagating large-flowered hydrangea "endless summer" by using sterile tissue cultured seedling leaves through a callus differentiation adventitious bud approach, and belongs to the field of agricultural biotechnology. Background technique [0002] Hydrangea [Hydrangea macrophylla (Thunb) Ser.], also known as hydrangea, hydrangea, etc., is an ornamental shrub of the genus Hydrangea in the family Saxifraga. Hydrangea is native to Japan and Sichuan, China, and is now cultivated all over the world. China is mainly distributed in provinces and regions south of the Yangtze River. Hydrangea not only has good ornamental effects but is also shade-tolerant. It can be cultivated as potted flowers, but also can be planted in courtyards, parks, and green spaces. It is an important ornamental plant in gardens. It is widely used in landscaping and beautification and has a large demand for seedlings. At the same time, hydrang...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 孙晓波邓衍明苏家乐陈双双齐香玉梁丽建
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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