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A method for rapidly breeding Hydrangea large-flowered Endless Summer with leaves of tissue-cultured seedlings

A technology of tissue culture seedlings and leaves, applied in the field of agricultural biology, can solve the problem of low callus induction rate, achieve the effects of increasing the reproduction coefficient, shortening the reproduction cycle, and increasing the value of scientific research and economic value

Active Publication Date: 2021-08-31
JIANGSU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to the above literature reports on inducing callus from hydrangea leaves, the inventor found through repeated experiments that the culture medium and culture conditions reported above are not suitable for Hydrangea grandiflora 'Endless Summer', and the leaves of tissue cultured seedlings of 'Endless Summer' were grown under the above conditions. lower callus induction rate

Method used

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  • A method for rapidly breeding Hydrangea large-flowered Endless Summer with leaves of tissue-cultured seedlings
  • A method for rapidly breeding Hydrangea large-flowered Endless Summer with leaves of tissue-cultured seedlings
  • A method for rapidly breeding Hydrangea large-flowered Endless Summer with leaves of tissue-cultured seedlings

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] 1, 'the endless summer' obtains a sterile tissue culture and proliferation

[0040] Clipping 'endless summer' 5 ~ 7cm long shoots emerging year (with 4-6 buds), wash with detergent and clean water, running water 2 to 3 hours. Under sterile conditions, first with 70% ethanol immersion for 30 seconds, and then 0.1% HgCl 2 Sterilized for 10 minutes, rinsed in sterile water 4-6 times, and inoculated onto MS medium, obtained after 30 days was cut about 2cm aseptic stem segments (with two lateral buds) is transferred to MS + 6 -BA propagated on 2.25mg / L + IBA0.1 mg / L media, then shoots 30 days after transfer to the proliferation MS + 6-BA1.0mg / L + IBA0.1 mg / L culture medium for seedlings.

[0041] 2, Leaves on callus induction of leaves bits

[0042] Step 1 were clipped 'endless summer' sterile tissue culture morphology 3 and 4, 5 and 6, 7 and 8, 9 and 10 blade, taken perpendicularly to the main cutting knife and vein to petioles and cutting leaves a slightly manner plate...

Embodiment 2

[0074] 1, 'Endless Summer', 'shy leaf' and 'Evita' get three large flower hydrangea varieties sterile tissue culture and proliferation

[0075] Respectively clipping 'endless summer', 'shy leaf' and 'Evita' 5 ~ 7cm long shoots emerging year (with 4-6 buds), wash with detergent and clean water, running water 2 to 3 hours. Under sterile conditions, first with 70% ethanol immersion for 30 seconds, and then 0.1% HgCl 2 Sterilized for 10 minutes, rinsed in sterile water 4-6 times, and inoculated onto MS medium, obtained after 30 days was cut about 2cm aseptic stem segments (with two lateral buds) is transferred to MS + 6 -BA the value-added 2.25mg / L + IBA 0.1mg / L medium, 30 days and then proliferation shoots transferred to the MS + 6-BA 1.0mg / L + IBA 0.1mg / L for seedling culture medium . Stem sections multiplication medium MS + 6-BA 2.25mg / L + IBA 0.1mg / L and seedling culture medium MS + 6-BA 1.0mg / L + IBA0.1 mg / L for three species of hydrangea flower ' endless summer '...

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PUM

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Abstract

The invention discloses a method for rapidly breeding hydrangea 'Endless Summer' by utilizing the leaves of tissue cultured seedlings, comprising: A) cutting the third to sixth leaves of the aseptic tissue cultured seedlings of 'Endless Summer', vertical to the leaves Cut a knife in the main vein and remove the petiole and leaf apex; B) place the leaves on the callus induction medium, and carry out light culture after dark culture; C) after light culture, transfer the callus leaves to callus proliferation and Carry out subculture on the adventitious bud induction medium to form clustered buds; D) cut the clustered shoots and transfer them to the adventitious bud growth medium for elongation and growth to form seedlings; E) cut the seedlings and transfer them to the seedling and On the rooting medium, the seedlings develop into complete regenerated plants with roots, stems and leaves; the present invention induces callus by adopting the leaves of the aseptic tissue cultured seedlings of 'Endless Summer', and differentiates adventitious buds from the callus, avoiding the During the tissue culture process of 'Endless Summer', the explants need to be sterilized and the contamination rate is high.

Description

Technical field [0001] The present invention relates to a method of using a sterile tissue culture callus differentiation blade by way of adventitious buds rapid propagation of large Hydrangea 'endless summer', which belongs to the field of agricultural biotechnology. Background technique [0002] Hydrangea [Hydrangea macrophylla (Thunb) Ser.], Also known as hydrangea, hydrangea, etc., as Saxifragaceae Hydrangea (Hydrangea genus that is) ornamental shrubs. Hydrangea native to Japan and China Sichuan area, now cultivated all over the world now, China is mainly distributed in the provinces south of the Yangtze River region. Hydrangeas is not only good shade and ornamental effect, both for flower cultivation, but also planted in gardens, parks, green spaces, the garden is an important ornamental plants in landscaping, beautification widely applied, the demand for large seedlings. At the same time the spent medical Hydrangea also has a high medicinal value, which was found hydrangea ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 孙晓波邓衍明苏家乐陈双双齐香玉梁丽建
Owner JIANGSU ACAD OF AGRI SCI
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