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A SNP molecular marker related with chicken carcass traits and application thereof

A carcass trait and molecular marker technology, applied in recombinant DNA technology, microbial determination/inspection, DNA/RNA fragments, etc., can solve problems such as low efficiency, accelerate genetic progress, save production costs, and have great economic application value and research value

Active Publication Date: 2019-01-11
SOUTH CHINA AGRI UNIV
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  • Claims
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AI Technical Summary

Problems solved by technology

At present, SNP search and screening is carried out by sanger sequencing or PCR-RFLP method. Due to the low efficiency of these technical methods, the rapid development of genechip technology and High-throughputsequencing in recent years can efficiently screen mutations at the genome level. site

Method used

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  • A SNP molecular marker related with chicken carcass traits and application thereof
  • A SNP molecular marker related with chicken carcass traits and application thereof
  • A SNP molecular marker related with chicken carcass traits and application thereof

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Embodiment

[0020] The chickens to be tested are F2 broiler chickens crossed by Xinghua chicken and recessive white rooque chicken. The number is 320. It is a fast and large broiler chicken, and Xinghua chicken is a broiler chicken native to Guangdong, China. Then the body weight (BW, Body Weight, in g) of the chicks was measured when they were 1 to 8 weeks old. Slaughter samples were taken at the age of 3 months, and their shin length, head width, chest width, chest depth, body length, chest horn width, slaughter weight, cortical thickness, visceral weight, whole half eviscerated weight, pectoralis leg were recorded respectively. Muscle weight, feather weight, abdominal fat weight, head and neck weight, heart weight, liver weight, stomach weight and small intestine length. The chest angle was measured with a thoracic horn; the subcutaneous fat thickness, shin length, fat width, head width, chest width, chest depth, and body length were measured with a vernier caliper; the genomic DNA of...

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Abstract

The invention discloses an SNP molecular marker related to chicken carcass traits and application thereof in breeding, wherein The SNP molecular marker corresponds to a mutation site in the gene sequence of the chicken let-7b precursor region. 526 G) A, and the genotype of the SNP site is AA, GA and GG. The SNP molecular marker of the present invention, that is, the mutation site in the gene sequence of the chicken let-7b precursor region g. 526 G)A is a new molecular marker associated with chicken carcass traits.. The early selection of carcass traits by determining the genotype of SNP locuscan save production cost and accelerate genetic progress. It can be used in chicken breeding and has great economic value and scientific research value.

Description

technical field [0001] The invention relates to the technical field of gene detection, in particular to a SNP molecular marker related to chicken carcass traits and its application. Background technique [0002] MicroRNA (miRNA) is a kind of endogenous non-coding single-stranded RNA with about 22 nucleotides. With the rapid development of molecular biology research techniques and methods, researchers have discovered more and more miRNAs. miRNA is produced by shearing a miRNA precursor (pre-miRNA) with a length of 70-80 nucleotides and containing a hairpin structure. MiRNA regulates the gene by complementing the 3' untranslated region (3'UTR) of its target gene mRNA, resulting in the inhibition of the translation of the mRNA. Although miRNA does not directly encode protein, the encoded RNA plays an important role in the whole life process of organisms. It has been found that miRNAs can participate in many regulatory pathways, including skeletal muscle development, virus de...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 罗文张细权吴静文陈庚华蒋明雅
Owner SOUTH CHINA AGRI UNIV
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