The invention discloses a method for building a standard CYP450 (
cytochrome p450)
gene type
database. The method comprises the steps as follows: comparing a specific sequence corresponding to
mutation information of a CYP450
gene type with a standard human whole
genome sequence to obtain a corresponding relation between the specific sequence and the standard human whole
genome sequence; and converting the CYP450
gene type into a
gene type taking the standard human whole
genome sequence as a reference sequence according to the corresponding relation. The invention further discloses a
database built by the method and a CYP450 gene
typing and enzymatic activity identification method based on the
database. The database is convenient for research of CYP450; the gene
typing method provides unified standards for CYP450 gene
typing and provides more accurate judgment criterions for diseases, drugs or the like; unknown
mutation sites in CYP450 genes can be effectively detected; hundreds of samples can be detected at the same time; and the coverage range is comprehensive and wide.