SNP (single nucleotide polymorphism) combination, detection method and kit for detecting liver damage susceptible genotype of antitubercular drug

Abstract

The present invention relates to an SNP (single nucleotide polymorphism) combination, detection method and kit for detecting liver damage susceptible genotype of an antitubercular drug and belongs to the technical field of medical molecular biological diagnosis; the SNP combination includes 7 SNP sites, and nucleotide sequences of the 7 SNP sites are shown sequentially as in SEQ ID NO. 1-7; the present invention also relates to an SNP detection method, comprising PCR (polymerase chain reaction) amplification and double-labeled probe melting curve analytical reaction, and primer pairs and double-labeled probe sequences for detection of the 7 SNP sites are shown as in SEQ ID NO. 8-20. The SNP site combination, detection method and kit provided herein enables quick, accurate, simple and high-throughput detection for a patient's genotype and prediction for the liver damage risk due to the patient using the antitubercular drug.

Description

technical field [0001] The invention relates to single nucleotide polymorphism (SNP) detection, in particular to a SNP combination, a detection method and a kit for detection of susceptibility genotypes of liver damage of anti-tuberculosis drugs, belonging to the technical field of medical molecular biology diagnosis. Background technique [0002] Tuberculosis remains a serious public health problem, especially in developing countries. According to WHO statistics, 1 / 3 of the world's population is infected with Mycobacterium tuberculosis (MTB), which is the second largest killer of infectious diseases in the world. my country is one of the 22 countries with a high burden of tuberculosis in the world, and the number of active tuberculosis patients ranks second in the world. At present, the infection rate of Mycobacterium tuberculosis in all age groups in my country is 44.5%, and about 550 million people in the country are infected by Mycobacterium tuberculosis. [0003] Curr...

AI Technical Summary

Problems solved by technology

The direct sequencing method is the "gold standard", but the operation is complicated, the cost is high, and the cycle is long; the chip method has high throughput and is suitable for the detection of large samples, but the accuracy is poor; the mass spectrometry method has high accuracy, but requires professional instruments and costs Higher; both Taqman method and high-resolution melting curve method are based on fluorescent quantitative PCR technology, which is simple to operate, but the throughput is low. Generally, only one SNP site can be detected in one reaction

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