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Primer composition for discriminating green-shelled-egg chicken genotype and use thereof

A green-shell laying hen and genotyping technology, which is applied in the field of primer composition identification of green-shell laying hens, can solve the problems of low breeding accuracy and elimination

Inactive Publication Date: 2015-08-12
SICHUAN AGRI UNIV
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AI Technical Summary

Problems solved by technology

The green-shell egg genotype (LS) is dominant relative to the non-green-shell egg genotype (CC), so the phenotype of the green-shell egg can appear as long as a chromosome single strand of the SLCO1B3 gene is contained in the somatic cells of the hen, and if If the rooster is heterozygous, theoretically half of the offspring will contain non-green egg genotypes, but this part cannot be eliminated in phenotypic breeding, so the selection accuracy is not high, so it is necessary to select molecular markers

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  • Primer composition for discriminating green-shelled-egg chicken genotype and use thereof
  • Primer composition for discriminating green-shelled-egg chicken genotype and use thereof
  • Primer composition for discriminating green-shelled-egg chicken genotype and use thereof

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Embodiment Construction

[0016] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention and the accompanying drawings. Obviously, the described embodiments are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0017] The experimental methods used in the following examples are conventional methods unless otherwise specified.

[0018] The materials and reagents used in the following examples can be obtained from commercial sources unless otherwise specified.

[0019] Using the chicken whole genome DNA containing the SLCO1B3 gene as a template, design a specific primer pair to amplify the EAV-HP insert sequence of the chicken SLCO1B3 gene, and perform the ...

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Abstract

The invention discloses a primer composition for discriminating a green-shelled-egg chicken genotype and a use thereof. The primer composition comprises two primer pairs, one primer pair comprises SLCO1B3 gene inserted EAV-HP sequence primers respectively shown in the formulas of SEQ ID NO. 1 and 2, and the other primer pair comprises SLCO1B3 gene non-inserted sequence primers respectively shown in the formulas of SEQ ID NO. 1 and 3. A DNA genome of a chicken to be detected is used as a template and is subjected to PCR amplification under the action of the primer pairs so that a PCR amplification product is obtained. Through detection of the amplified 209bp and 312bp bands, it is determined if the chicken to be detected can produce green-shelled eggs. The primer composition can discriminate the green-shelled-egg chicken genotype.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a primer composition for identifying green-shell layer hens and an application thereof. Background technique [0002] Molecular marker assisted selection (MAS, marker assisted selection) is a new technology produced with the rapid development of modern molecular biology technology. Selection, molecular breeding. At present, the application of MAS technology mainly focuses on gene pyramiding, gene transgression, and the construction of gene lines according to breeding programs. In poultry breeding, molecular auxiliary marker selection is widely used. For example, the phenotypic selection of feather speed, shank length, and bare neck traits is very successful, and it is widely used and produced, and the corresponding fast and slow feather genes (Kk), sex-linked dwarf genes (dwarf, dw) and bare neck genes as molecular The technology of marking for MAS has also been gradually applied ...

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Application Information

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IPC IPC(8): C12N15/11C12Q1/68
Inventor 赵小玲李思辰舒刚朱庆张露崔璨尹华东王彦刘凌斌竭航
Owner SICHUAN AGRI UNIV
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