The invention belongs to the technical field of chicken molecule mark preparation, and in particular relates to and builds a method for identifying molecules of recessive white
feather genes of chicken. In the method for identifying the molecules of the recessive white
feather genes of the chicken,
nucleotide sequences of recessive white
feather control genes of the chicken (TYR genes inserted with retroviruses) and
colored feather control genes TYR genes are utilized to design a primer pair 1 and a primer pair 2 respectively, wherein the primer pair 1 is used for detecting recessive white feather allelic genes; and the primer pair 2 is used for detecting
colored feather allelic genes. The method comprises the following steps: extracting
genomic DNA of the chicken to be identified and performing PCR amplification by using the primer pairs 1 and 2; performing
electrophoresis detection on amplification products; judging the type of the feather color genes of the chicken according to
electrophoresis results; if the amplification products are produced by only the primer pair 1, determining the feather color genes are recessive white feather homozygote (the
phenotype is a white feather); if the amplification products are produced by only the primer pair 2, determining the feather color genes are non-recessive white feather homozygote (the
phenotype is either a
colored feather or the white feather); and if the amplification products are produced by both of the primer pairs 1 and 2, determining the feather color genes are recessive white feather heterozygote (the
phenotype is either a colored feather or the white feather). The invention provides the quick and convenient method for identifying the molecules of the recessive white feather genes of the chicken.