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61results about How to "Conservation of Germplasm Resources" patented technology
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Method for promoting propagation of tricholoma matsutake artificially
InactiveCN101861795AProtect the environmentEffective sustainable developmentHorticultureTricholoma matsutakeSpore
The invention relates to a method for promoting the propagation of tricholoma matsutake artificially and belongs to the technical field of edible fungus germplasm resource protection and utilization, in particular to innovative technology which is used for realizing stable and high yield of the tricholoma matsutake by using 'tricholoma matsutake mountain' forestry management and adopting manual propagation promoting measures in a special environment needed for growing the tricholoma matsutake in order to improve the fact that the artificial culture of the tricholoma matsutake by using purely cultured stains is a goal difficult to fulfill for a long time. The technical scheme of the invention mainly comprises propagation promoting technology using tricholoma matsutake spores, mycorrhizae and hyphae as propagation materials, forestry management tricholoma matsutake propagation promoting technology and fungus shiro artificial tricholoma matsutake propagation promoting technology. The method not only is a tricholoma matsutake native area conservation and propagation promoting measure, but also can protect the shiro, promote the sound growth of the shiro and promote the yield and quality of tricholoma matsutake.
Owner:YUNNAN AGRICULTURAL UNIVERSITY
Tissue culture and propagation method and inductive culture mediums for Chongzuo camellia nitidissima
InactiveCN102972291ARapid and efficient reproductionPromote germinationHorticulture methodsPlant tissue cultureGermplasmCulture mediums
The invention discloses a tissue culture and propagation method and inductive culture mediums for Chongzuo camellia nitidissima. According to the method, the improved MS, 6-benayl aminopurine (6-BA) and 3-indoleacetic acid (IAA) are used as an inductive culture medium for the cluster buds of Chongzuo camellia nitidissima; and the combination of the two growth hormones can be used for activating acting enzymes in the body cells of Chongzuo camellia nitidissima, and promoting the germination and the growth of the explants of Chongzuo camellia nitidissima in a proper concentration and a weakly acidic environment; the thick and strong cluster buds can be formed after the culturing for 55 days, and the propagation rate is increased by 20 times. According to the method disclosed by the invention, 1 / 2 improved MS and indolebutyric acid (IBA) or 1 / 2 improved MS and napthaleneacetic acid (NAA) are further used as an inductive culture medium for rooting, and capable of effectively promoting the single seedlings of Chongzuo camellia nitidissima to root to form new plants. Via the tissue culture and propagation method applying the three inductive culture mediums for Chongzuo camellia nitidissima, Chongzuo camellia nitidissima can be rapidly and effectively propagated, thus solving the problems that wild resources are diminished gradually and the natural propagation rate of Chongzuo camellia nitidissima is low, and effectively protecting the precious plant genetic resource which is rare in the world.
Owner:GUANGXI FORESTRY RES INST
Method for producing selenized dendrobium huoshanense culture
ActiveCN102835317AConservation of Germplasm ResourcesLow costPlant tissue cultureHorticulture methodsDendrobium huoshanensePesticide residue
The invention discloses a selenized dendrobium huoshanense culture and a method for producing the selenized dendrobium huoshanense culture. The method comprises the steps of: inducing to obtain an aseptic seedling of dendrobium huoshanense, breeding to obtain callus of selenium-resistant dendrobium huoshanense by collaboration of dark and low-salt medium, domesticating the selenized dendrobium huoshanense callus by the low-salt medium assisted by blue light, and producing the selenized dendrobium huoshanense culture by illumination of a fluorescent lamp. The mass fraction of organic selenium in dried product of the selenized dendrobium huoshanense culture produced by the method described by the invention is not less than 50mug / g. According to the method provided by the invention, high-quality dendrobium huoshanense substitute without hormone and pesticide residue can be produced at low cost and large scale via standardization; the selenized dendrobium huoshanense culture product has activity quality of the dendrobium huoshanense and the organic selenium, and can be applied to medicines and health-care products.
Owner:安徽省华信生物药业股份有限公司
Method for producing taxol by utilizing Chinese yew seed endophyte
Owner:LIAOCHENG UNIV
Breeding technology for large-size parents of Eriocheir sinensis
ActiveCN103026989APromote steady developmentStable and sustainable useClimate change adaptationPisciculture and aquariaMatingSarcocheilichthys sinensis
The invention relates to a breeding technology for large-size parents of Eriocheir sinensis. The breeding technology comprises the step of selecting the athletic parents of the Eriocheir sinensis, wherein the size of the female crab is above 150g and the size of the male crab is above 200g; the breeding technology is characterized by comprising the following steps: from the last ten-day of October to the first ten-day of November per year, soaking the selected parents of the Eriocheir sinensis into fresh water with tiny running water and sufficient oxygen for 2-3 minutes, and then taking out; after 3-5 minutes, disinfecting by using potassium permanganate; temporarily breeding in an indoor glass reinforced plastic over-wintering pool; starting the over-wintering breeding for the parents of the Eriocheir sinensis in the middle ten days of December; at the beginning of March per year, at a mating moment, controlling the water temperature at 9-12 DEG C, wherein the male and female ratio is (1.5-2):1 and the mating density is 7-13 pieces / m<2>; after oogenesis, continuously aerating and increasing the inflating volume, wherein the breeding temperature for the larva of the Eriocheir sinensis is at 24-25 DEG C and the breeding seawater salinity is 1.8%; and feeding rotifera, fairy shrimp larva and fresh water cladocerans biological baits.
Owner:上海市水产研究所(上海市水产技术推广站)
Grafting cultivation method for zeldova serrata seedlings
InactiveCN105123410AImprove disease resistanceImprove survival rateCultivating equipmentsHorticultureSurface moistureHuman waste
The invention provides a grafting cultivation method for zeldova serrata seedlings. The method comprises the following steps that 1, in January to February of every year, annual strong branches of local wild zeldova serrata of more than 30 years old are selected as scions, and a solution containing a disinfectant is adopted to perform disinfection treatment when grafting is performed; 2, seeds are collected from zeldova serrata seed trees and soaked with a germination accelerating solution for 2 days before being sown, then the surface moisture of the seeds is aired to wait for being sown, and seedlings can be used as stocks when the ground diameter of the seedlings is more than 5 cm; 3, in the southern mountainous area in Anhui Province, a land parcel which is deep in soil and rich in humus is selected to be reclaimed, flattened and then covered with 20 centimeters of humus soil; 4, needle graft is adopted as a grafting method, the scions are fixed by winding insulating tape after being grafted, and short dendritic buds of the scions are exposed; 5, human wastes are additionally applied in time when the seedlings are survived and grow to 30 cm, a leaf surface sterilization solution is sprayed once a month until the seedlings grow to higher than 1 m, and weeding is performed without specific time limitation and performed as long as grass grows; 6, daily management is performed according to a conventional zeldova serrata management method.
Owner:郎溪县韵林苗木种植专业合作社
Method for inducing callus of lycoris radiate by using flower stalks
InactiveCN102405841AAlleviate the problem of high pollution rateHigh induction success rateHorticulture methodsPlant tissue cultureSaccharumSaccharophagus degradans
The invention discloses a method for callus of lycoris radiate by using flower stalks, comprising the following steps: taking the flower stalks of the lycoris radiate after being sterilized; inoculating in induction culture medium, cultivating for 28-32 d in sunshine, then moving to a dark environment, and cultivating for 88-92 d, wherein the induction culture medium comprises 2.0-2.5 mg / L of 2,4-D and 1.5-2.5 mg / LBA of MS culture medium, and the MS culture medium comprises sugarcane of which concentration is 30-60 g / L and agar of which concentration is 5-8 g / L and pH is 5.6-5.8. The invention adopts the flower stalks of the lycoris radiate as the exophyte, produces the callus of the lycoris radiate through cultivating in the induction culture medium, cannot be at the cost of consuming the bulbs, and effectively protects the genetic resources of the rare lycoris radiate which has good quality.
Owner:ZHEJIANG UNIV
Lepista sordida and separation and propagation method and soil covering cultivation method thereof
ActiveCN108293599AMaster the multiplication methodConservation of Germplasm ResourcesFungiMicroorganism based processesMicroorganismGermplasm
The invention discloses lepista sordida and a separation and propagation method and soil covering cultivation method thereof. N006# strains are obtained by tissue separation of wild lepista sordida fruiting bodies, and the lepista sordida N006# strains are preserved in China General Microbiological Culture Collection Center (CGMCC); the preservation date is June 1st, 2016; the preservation registration number is CGMCC NO. 12507. The lepista sordida N006# strains are obtained through tissue separation, a propagation method of the strains is mastered, and strain guarantees are provided for artificial cultivation of lepista sordida. The lepista sordida fruiting bodies are obtained through artificial soil covering cultivation, germplasm resources of this specie are protected, and a novel edible fungus variety is developed.
Owner:KUNMING INST OF EDIBLE FUNGI CHINA NAT SUPPLY & MARKETING GENERAL COOP
Rice yield increasing planting method
The invention belongs to the technical field of rice planting, in particular to a rice yield increasing planting method which increases the yield through intervarietal mixed cultivation. According to the rice yield increasing planting method, rice varieties with different resistance properties are fully combined to form a stable ecological system suitable for rice to survive, and the high yield of rice is realized through potential maximization. According to the rice yield increasing planting method, rice varieties with similar growth periods, similar agronomic traits and different resistance properties are selected for raise rice seedlings, and then the rice seedlings are mechanically transplanted at the same period. The different resistance properties of the rice varieties interact to give full play to the production potential of all the rice varieties; meanwhile, a three-dimensional community is built, so that stability of a field ecological system is improved, common diseases, pests and weeds are effectively controlled without applying pesticides or applying a small number of pesticides, and green production of rice is realized.
Owner:CHINA NAT RICE RES INST
Early maturing culture method for Tai lake water shield
InactiveCN105746148APromote maturityImprove mu benefitsPlant cultivationCultivating equipmentsChemical controlGermplasm
The invention discloses an early maturing culture method for Tai lake water shield. The method comprises the steps of site selection, field selection, greenhouse building, selection of varieties and seedlings, air temperature ventilation and water level management in the field, pest disease and weed prevention and control, harvesting and treating, wherein the pest disease and weed control is performed in a manner of combining agricultural control and chemical control. According to the culture method disclosed by the invention, the water shield can be picked in advance, benefits of the Tai lake water shield per mu can be increased, the planting enthusiasm of peasants can be improved, germplasm resources of the Tai lake water shield are protected, and sustainable development of the industry is protected; moreover, the pest disease and weed control is performed by a method of combining agricultural control and low-toxicity agent control, so that water body pollution is reduced, a green control aim is achieved, and the safety is high.
Owner:SUZHOU DONGSHAN AGRI DEV CO LTD
Cultivating method of Gymnocypris przewalskii spring fingerlings
InactiveCN102187835AConservation of Germplasm ResourcesGood economic and social environmental ecological benefitsClimate change adaptationPisciculture and aquariaPhacusDisease
The invention belongs to the technical field of cultivation of Gymnocypris przewalskii, and in particular relates to a cultivating method of Gymnocypris przewalskii spring fingerlings. The scheme of the method comprises the four steps of changing feeding points, managing water quality, using a feeding method and cultivating spring fingerlings. Until June, the cultivated spring fingerlings can reach 9-11 cm in size and 7.0-8.0 g in weight, are 1+ age Gymnocypris przewalskii spring fingerlings, can be purchased or proliferated for releasing and can also enter the 2+ age fingerling cultivation stage. The method provides powerful support for the cultivation of Gymnocypris przewalskii, the germ plasm resource protection of Gymnocypris przewalskii, satisfaction of the market demand of Gymnocypris przewalskii, and the cultivation of Gymnocypris przewalskii in fresh water of cold areas. The method has the advantages that: being different from the cultivation modes of spring fingerling breeding of other cultured fishes, cultivation in the original pond is adopted; the strongly-cultivated fingerlings have high survival rate and less diseases; and simpleness and convenience for operation and labor saving are achieved.
Owner:史建全
Amplification primer and method of nassariidae mitochondria COI gene
InactiveCN104388571AEfficient amplificationThe amplification efficiency of the amplification primers is effectiveMicrobiological testing/measurementDNA/RNA fragmentationSpecies classificationToxin
The invention belongs to the field of molecular biology, and provides an amplification primer of a nassariidae mitochondria COI gene, and a method for amplifying the nassariidae mitochondria COI gene by using the primer. According to the amplification primer of the nassariidae mitochondria COI gene, the sequences are ZLCO1490F:5'-TTTCTACAAATCATAAAGACATTGG-3' and ZHCO2198R:5'-TATACCTCCGGGTGACCAAAAAATCA-3'. The amplification primer of the nassariidae mitochondria COI gene provided by the invention is capable of effectively amplifying COI sequences of different species of the nassariidae, and has great significance in research of species classification, system development, systematic geography, toxin tolerance and protection of nassariidae genetic resources by using the COI sequences.
Owner:NANJING AGRICULTURAL UNIVERSITY
Parabotia fasciata larva breeding method
ActiveCN105432524AConservation of Germplasm ResourcesGood for healthFood processingClimate change adaptationGermplasmWater quality
The invention provides a parabotia fasciata larva breeding method, and relates to a freshwater fish larva breeding technology. The method comprises the steps that parabotia fasciata larvae are bred in a larva breeding pool; artificial and feed domestication treatment is conducted on the larvae, water quality adjustment is conducted, breeding is conducted for 15 days, and parabotia fasciata larva fish with the body length ranging from 0.6 cm to 1.0 cm can be obtained; the fish are transferred to a fingerling breeding pool, bait is put into the fingerling breeding pool, water quality adjustment is conducted on breeding water, dissolved oxygen of the breeding water of the fingerling breeding pool is larger than 5 mg / L, the ammonia nitrogen content is smaller than 0.01 mg / L, breeding is conducted for three months, and parabotia fasciata fingerling with the body length ranging from 5 cm to 6 cm can be obtained. According to the parabotia fasciata larva breeding method, parabotia fasciata larva and fingerling breeding technical problems can be solved, batched production of the parabotia fasciata fingerling is achieved, parabotia fasciata germplasm resources are protected, the survival rate of the parabotia fasciata fingerling bred through the method reaches 80%-85%, and the fingerling survival rate reaches 60%-70%.
Owner:NEIJIANG NORMAL UNIV
In-vitro propagation method of plant Hydrocera triflora
ActiveCN108901845ASimple recipeWide range of ingredientsPlant tissue cultureHorticulture methodsAdditive ingredientBud
The invention belongs to a new plant or a method for obtaining a new plant and the technical field of plant regeneration by tissue culture technology, and discloses an in-vitro propagation method of plant Hydrocera triflora. The in-vitro propagation method of the plant Hydrocera triflora comprises seed collection and disinfection; seed germination and bud induction culture; bud proliferation subculture; rooting and strong seedling culture. The method has great advantages: 1. a small amount of seeds are needed; 2. the proliferation coefficient is large, and the bud proliferation coefficient reaches 8.00 in 30 days of culture; 3. rooting and strong seedlings are good, the rooting rate is 100%, and the number of roots is as high as 6.42; 4. a developed medium has a simple formula and a wide range of ingredients, and is easy to operate and formulate; 5. the breeding of seedlings can be produced annually without being limited by time and seasons.
Owner:海南省农业科学院热带园艺研究所
Three-grade branch pruning technology of fructus evodiae
InactiveCN104542038AIncrease productionImprove qualityCultivating equipmentsHorticulture methodsTetradium ruticarpumYoung tree
The invention discloses a three-grade branch pruning technology of fructus evodiae. The three-grade branch pruning technology comprises the following steps: carrying out tip pruning on branches near the ground of fructus evodiae young trees with the plant spacing of 3*4m and planted for 1-2 years; controlling the branches to be dwarfed so that primary braches are gradually divided into stem branches; carrying out tip pruning on the stem branches after autumn or spring to form secondary branches; finally, carrying out tip pruning to form tertiary branches; pruning the secondary branches and the tertiary branches; pruning dense branches, overlapped branches, downward branches, insect branches and the like; and continuously pruning and managing and enabling whole plants to form tree shapes which have short stems and low crowns, circular outsides and hollow insides and wide tree crowns, are ventilated and transparent, and have the tree heights controlled to be lower than 1.5m and the tree crown coverage of being more than 10 square meters within 3-4 years. According to the three-grade branch pruning technology, the fructus evodiae can be subjected to three grades of branch pruning so that the quality and yield of cultivation of the fructus evodiae are effectively improved and the labor cost is reduced; the germplasm resources of the fructus evodiae are effectively protected; the three-grade branch pruning technology has remarkable social and economic benefits and has a wide application prospect.
Owner:湖南湘泉药业股份有限公司
Quick red bean local variety purifying and rejuvenating method
InactiveCN108124718ASpeed up the purification and rejuvenation processExcellent breedFabaceae cultivationResource protectionGermplasm
The invention discloses a quick red bean local variety purifying and rejuvenating method and relates to the technical field of crop variety resource protection and utilization. The method comprises the steps: performing large group selection to select single plants and performing plant laboratory test and forming plant rows; identifying and comparing multiple plant rows and forming plant lines through the plant laboratory test; comparing multiple plant lines to predict yield and selecting high-yield plant systems through the plant laboratory test; performing mixed line breeding and producing qualified seeds. The method disclosed by the invention widens selected groups, can perform plant laboratory test according to red bean representations, ensures population genetic stability, reduces gene loss generation, quickly increases population quantity and increases output quantity of breeder seeds; seed propagation generations are reduced, a mixing opportunity is reduced, and a natural selection effect is reduced; meanwhile, a red bean local variety purifying and rejuvenating process is quickened, excellent variety characteristics are kept, germplasm resources with local characteristics are effectively protected, and the breeder seeds can be directly applied to large field production or the breeder seeds can be utilized to directly produce seeds.
Owner:德州市农业科学研究院
Amplification primer of psammobiidae shellfish mitochondria COI (Cytochrome Oxidase I) gene
InactiveCN102776182AConservation of Germplasm ResourcesLow amplification efficiencyDNA/RNA fragmentationDesign softwarePsammobiidae
The invention relates to an amplification primer of a psammobiidae shellfish mitochondria COI (Cytochrome Oxidase I) gene. 64 psammobiidae sequences obtained by amplification of universal primers are subjected to comparative analysis by using CLUSTALW software; a plurality of pairs of primers are designed by using primer design software Primer Premier 5 in a determined conserved region; PCR (Polymerase Chain Reaction) reaction experiments are repeatedly performed by using 200 psammobiidae individuals under a 10 mu system; and finally an efficient amplification primer of a psammobiidae shellfish mitochondria COI gene sequence is sifted through detection of agarose gel electrophoresis. The amplification primer is characterized in that COIZGF is 5'-ACAAATCATTAAGGATATTGG-3'; and COIZGR is 5'-GTCCGAAGAATCAAAACAAATG-3'. With the adoption of the primer of the psammobiidae shellfish mitochondria COI, provided by the invention, target segments of different species of psammobiidae shellfishes can be effectively amplified, the amplification efficiency is more than 90%, the primer can satisfy the demands of genetic diversity research on psammobiidae, and plays an important role in the aspects of protection and utilization of psammobiidae resources, diversity assessment, genetic breeding and the like.
Owner:OCEAN UNIV OF CHINA
Lepista nuda strain and sporocarp cultivation method thereof
ActiveCN108770593ARich Variety ResourcesConservation of Germplasm ResourcesCultivating equipmentsMushroom cultivationEdible mushroomLepista
The invention discloses a lepista nuda strain and a sporocarp cultivation method thereof. The invention provides Lepista nuda(Lepista nuda DCH 560), and the deposit number is CGMCC No. 15376. The Lepista nuda DCH 560 (CGMCC No. 15376) is collected from a specimen isolated from the specimen of Dandong Kuandian Manchu Autonomous County, Liaoning Province, China. The strain can be obtained by artificial domestication. The sporocarp cap obtained by cultivation is purple, the flesh is thicker, the pleats are purple, and it has a strong aroma, which is consistent with the wild sporophore. The invention has obtained thesporophore of the lepista nuda and developed a new rare edible fungus, which is of great significance for protecting the germplasm resources of the species, enriching the edible mushroom variety resources, enriching the vegetable basket and the like. At the same time, the bacterium is a typical grass rot fungus and the crop stalks can be utilized, which has ecological significance.
Owner:INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
Novel method for breeding kelp seedlings with gametophyte cloning method
InactiveCN102487820AShorten the growth cycleQuality improvementPlant tissue cultureHorticulture methodsSeparation technologyFemale Gametophytes
The invention provides a novel method for breeding kelp seedlings with a gametophyte cloning method, comprising the following steps of: respectively separating a female cell on a female gametophyte and a male cell on a male gametophyte under a microscope by using a capillary tube separation technology; then respectively cloning the female cell and the male cell by using a cloning vegetative propagation technology to respectively grow the female cell and the male cell into female and male gametophytes; continually breeding the gametophytes; when the gametophytes are bred to a fixed amount, damping a formed cell cluster by a cell stamping machine; mixing the female and male gametophytes and respectively releasing ova and sperms by the female and male gametophytes to spontaneously fertilize;putting fertilized zygotes into nutritive salt to be cultured; and after 10 weeks, obtaining seedlings with the height of about 1.5 cm. According to the invention, a fertilization process and the control of temperature and illumination are realized by manual operation, so that the growth period of offspring seeds is greatly shortened; meanwhile, the female cell and the male cell are separated from the gametophytes by using the capillary tube separation technology to clone and culture, so that the quality and the yield of the seedlings are improved, the cultivation of the kelp can be satisfied; and by utilizing the novel method, the cost for breeding the kelp seedlings is low.
Owner:YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
Industrialized production method for tricholoma matsutake edible fungi
InactiveCN108293614ARealize artificial domesticationConservation of Germplasm ResourcesCultivating equipmentsMushroom cultivationBiotechnologySocial benefits
The invention relates to an industrialized production method for edible fungi, belongs to the field of agricultural biotechnologies, and particularly relates to an industrialized production method fortricholoma matsutake edible fungi. The method comprises the following steps: strain production, material preparation, material steaming, cooling, inoculation, bagging, management in a fungus growth period, management in the process of producing the edible fungi, and performing harvesting. The method provided by the invention realizes artificial domestication of tricholoma matsutake mother strains, completely changes a current situation that tricholoma matsutake production completely depends on nature, industrially produces the edible fungi, and realizes stable production, a high yield and excellent quality of tricholoma matsutake products; the method effectively protects the tricholoma matsutake germplasm resources and a living ecological environment on which the tricholoma matsutake germplasm resources depend, and provides an effective way for sustainable development of tricholoma matsutake production; and the method has outstanding economic and social benefits.
Owner:陈晓红
Method for long-term storage of berberis heteropoda seeds
The invention discloses a method for long-term storage of berberis heteropoda seeds. The method comprises the following steps that the seeds are sterilized, disinfected and aired, and the dried berberis heteropoda seeds are placed under a 2-5 DEG C condition and soaked in a mixed solution comprising 15% of ethanediol, 10% of dimethyl sulfoxide (MSO) and 10% of cane sugar for 45-60 minutes; prefrozen berberis heteropoda seeds are placed into an absorbent gauze bag, and the berberis heteropoda seeds and the gauze bag are placed into a dryer with the lower layer being made of silica gel for drying; and the dried seeds are uniformly smeared with ceresin, packaged with a tinfoil bag airtightly, placed into a low-temperature environment with the temperature being 25-40 DEG C to be stored for 30-60 minutes, and then packed into a sealed bag which is filled with liquid nitrogen and has the temperature of -196 DEG C for cryopreservation. By adoption of the method, the activity of the seeds can be preserved in a long term, the rate of emergence of the seeds is guaranteed and increased, and the seedling establishment capability is improved.
Owner:FOREST ECOLOGICAL RES INST XINJIANG ACADEMY OF FORESTRY
Accelerated breeding method for licorice seeds
InactiveCN105557285AImprove germination ratePromote germinationPlant cultivationCultivating equipmentsDrip irrigationGermplasm
Provided is an accelerated breeding method for licorice seeds. The licorice fruit bearing period is shortened, and enough seeds are provided for artificially planting licorice. The accelerated breeding method for licorice seeds comprises the steps that triennial licorice principle roots are taken as licorice seedlings; a land parcel with irrigation arable soil or sandy soil is selected; the licorice seedlings are planted in a wide and narrow row spacing planting method; after the licorice seedlings are planted, a drip irrigation zone is laid beside a licorice seedling zone, and covered with a black mulching film; in the key breeding period of the licorice seedlings, drip irrigation water is applied once every 20 days, the drip irrigation amount is 20 m<3> each mu each time, and the number of total drip irrigation times in a year is 8-10; after 80% licorice pods in the licorice seed land are mature, a hay mower is used for harvesting at a time, and threshing and airing are carried out in time. By means of the breeding method, the breeding period of licorice can be 2-3 years earlier, the seed yield is increased by 2 times or above, the worm damage rate of the seeds is controlled to be within 5% and is reduced by 15% or above compared with that of wild seeds, the seed germination rate is greatly increased, and the seed germination potential is greatly improved. Meanwhile, the pressure that licorice seeds rely on wild seeds for a long time can be relieved, the wild licorice genetic resource can be protected, and artificial destruction on the environment is controlled.
Owner:甘肃巨龙供销(集团)股份有限公司 +1
Fish breeding and catching method for ecological circulation type fishery
InactiveCN110663594AConservation of Germplasm ResourcesImprove survival rateClimate change adaptationPisciculture and aquariaEutrophicationGermplasm
The invention discloses a fish breeding and catching method for ecological circulation type fishery, and belongs to the field of fishery; the fish breeding and catching method for ecological circulation type fishery comprises the steps, for example: selecting water areas, arranging fish blocking facilities, cultivating fingerlings, discharging the fingerlings out of ponds, breeding the fingerlingson water and recapturing the bred fingerlings. The survival rate of the fingerlings can be effectively increased after the fingerlings leave the ponds and enter the field environment; the fishery resources can be restored, the fish germplasm resources can be protected, and stable income is created for fishermen; the method has the advantage of simultaneous protection and utilization. According tothe method, the degraded fish resources of the lakes can be updated, the fish population structures are enriched, the total amount of the fish resources is increased, the sustainable utilization of the commodity fish resources is guaranteed, the eutrophication level of the lakes is reduced, and the ecological circular development of fishery is facilitated.
Owner:HUNAN UNIV OF ARTS & SCI
Cymbidium sinense 'labellum and petal sepalody' character-related functional molecular marker and identification method thereof
ActiveCN106755526AEfficient identificationAccurate identificationMicrobiological testing/measurementDNA/RNA fragmentationNucleotideGermplasm
The invention provides a cymbidium sinense 'labellum and petal sepalody' character-related functional molecular marker. The functional molecular marker is M4wt or M4m, the nucleotide sequence of the M4wt is shown in SEQ ID NO: 1, and the nucleotide sequence of the M4m is shown in SEQ ID NO: 2. By separating and comparing related gene sequences of multiple varieties of cymbidium sinense, a functional specificity site, which is different from other varieties of cymbidium sinense, in 'labellum and petal sepalody' varieties 'Lvyun' and 'Taiyanghua' is obtained. Based on difference of the site, three specific primers M4F1, M4F2 and M4R are designed. A primer pair of M4F1 and M4R and a primer pair of M4F2 and M4R are utilized to amplify the total DNA of the genome of the cymbidium sinense, then agarose gel electrophoresis detection is performed, and thus the 'labellum and petal sepalody' varieties can be quickly identified. The method provided by the invention has great value and meaning in identification and protection of germplasm resources of cymbidium sinense, molecular marker-assisted breeding and molecular design breeding.
Owner:SUN YAT SEN UNIV
Technique for processing plant under low temperature by polygonum cuspidatum callus
InactiveCN106342685AConservation of Germplasm ResourcesExcellent Germplasm ResourcesDead plant preservationPlant tissue cultureGermplasmPOLYGONUM CUSPIDATUM
The invention discloses a technique for processing plant under low temperature by polygonum cuspidatum callus. A callus obtained by induction of a polygonum cuspidatum seed is used as a material, an in-vitro ultra-low temperature storage technique system of polygonum cuspidatum is set up, thus high-quality germplasm resource of polygonum cuspidatum is effectively protected. Through callus induction, pre-cultivation, loading, dehydration, cooling, washing, re-cultivation and regeneration, and other processes, the ultra-low temperature storage of the polygonum cuspidatum callus is realized; the technique can be used as a method for stably storing germplasm resources for a long term, so as to reduce the economic cost for storage, and effectively protect rare germplasm resources.
Owner:李志勇
A method for producing paclitaxel by using yew seed endophyte
The invention provides a method for producing taxol by utilizing Chinese yew seed endophyte. The method for producing the taxol by utilizing the Chinese yew seed endophyte comprises the following steps: sterilizing and inoculating while Chinese yew seeds are taken as explants, then carrying out secondary sterilization, culturing for a period of time, picking endophytic fungi at the periphery of the Chinese yew seeds, placing the endophytic fungi into a PDA solid culture medium, culturing, then picking part of bacterial plaque, placing the picked bacterial plaque into PDA liquid and culturing; filtering ferment with gauze, and extracting filtrate with ethyl acetate; carrying out reduced-pressure distillation at the temperature of 35 DEG C after filtering by adopting a rotary evaporator, and dissolving in a small amount of methyl alcohol, so that a methanol solution containing taxol is obtained. The method for producing the taxol by utilizing the Chinese yew seed endophyte has the advantages that the problems that Chinese yew plants grow slowly and chemical synthesis of the taxol is complex while a fungus culturing technique is applied can be solved, a foundation is provided for industrial production of the taxol by fungal fermentation, the method for producing the taxol by utilizing the Chinese yew seed endophyte is environment-friendly, raw materials are rich, and the problem of high supply shortage of the taxol can be well relieved.
Owner:LIAOCHENG UNIV
Method for inducing callus of lycoris radiate by using flower stalks
InactiveCN102405841BAlleviate the problem of high pollution rateHigh induction success ratePlant tissue cultureHorticulture methodsSaccharumGermplasm
Owner:ZHEJIANG UNIV
How to liberate eyebrow crabs
ActiveCN108124804BConservation of Germplasm ResourcesImprove survival rateClimate change adaptationAgricultural fishingFisheryWater quality
Owner:日照市海洋与渔业研究所
A kind of in vitro propagation method of plant water horn
ActiveCN108901845BSimple recipeWide range of ingredientsHorticulture methodsPlant tissue cultureSeedlingCulture mediums
Owner:海南省农业科学院热带园艺研究所
A device and method for large-scale establishment of families of piriform snails
ActiveCN108617559BImprove reproductive efficiencyQuality improvementClimate change adaptationPisciculture and aquariaAnimal scienceFishery
The invention relates to a device and method for establishing a bellamya purificata family on a large scale. The device comprises bellamya purificata climbing rods, supporting surfaces and bellamya purificata cages; and the bellamya purificata climbing rods are used as stand columns to support the whole device, supporting surfaces are connected with the bellamya purificata climbing rods in a sleeving manner, the upper and lower ends of the bellamya purificata climbing rods are fixed, the bellamya purificata cages are mutually overlapped and placed between adjacent supporting surfaces, and theupper and lower ends of each bellamya purificata cage combination separately abut against the adjacent supporting surfaces. According to needs, each group of device is equipped with M bellamya purificata cages, N groups of devices are arranged to construct M*N families, and offsprings of each female bellamya purificata are raised in one same bellamya purificata cage. According to the device and method disclosed by the invention, a plurality of bellamya purificata families can simultaneously constructed according to needs, the device occupies less land and has a high space utilization rate, young bellamya purificata has a high survival rate and grows well, and the requirements for large-scale establishment of the bellamya purificata family are met; and meanwhile, female individuals and maleindividuals are raised separately, mating frequency of the male individuals is controlled, so that consumption of excellent male individuals is reduced, precious germplasm resources are protected, and the reproductive efficiency and offspring quality of the bellamya purificata are improved.
Owner:FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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