34results about How to "Excellent Germplasm Resources" patented technology

The invention provides a special cultivation substrate and an application method for solving the problems that root system recovering and reestablishment are difficult after ex-situ transplantation of alpine rose old tree stumps. The method comprises the preparation steps of A, self preparing leaf mold, wherein wood bits are added in collected dead leaves, the ratio of the wood bits to the collected dead leaves is 1:5, the mixture of the collected dead leaves and the wood bits are smashed, water is added in the mixture of the collected dead leaves and the wood bits to drench the mixture of the collected dead leaves and the wood bits, the mixture of the collected dead leaves and the wood bits are buried in the soil for one year and are sufficiently decomposed, and the leaf mold is prepared; B, using turfy soil, the self-prepared leaf mold and pine needles according to the volume ratio of 7:2:1 for preparing substrate raw materials; C, preparing mixed base fertilizers, wherein three kinds of controlled-release fertilizers with the ratios of N to P to K being 10:11:18, 15:9:11, and 9:14:19 respectively are selected, and the three kinds of release fertilizers are evenly mixed in the proportion of 1.5: 1: 2; D, evenly mixing the substrate raw materials, and then adding the prepared 1 kg/m<3> of base fertilizers in the mixed substrate raw materials; E, conducting water purification process; F, using the water processed in the step E for wetting the substrate, and evenly stirring the substrate again, wherein the substrate materials are held into a spherical shape by hands and immediately loosen if the hands let the substrates go, the proper moisture content is 80-90 %, and the standards of loosening, ventilation and water retention are met.

The invention discloses a method for breeding sexual triploids of colored zantedeschia by using 2n pollen, and belongs to the technical field of plant ploidy breeding. The method comprises the following steps of: (1) determining a 2n pollen induced appropriate development stage; (2) preparing styles to be treated; (3) inducing the 2n pollen by colchicine treatment; (4) identifying the 2n pollen; (5) collecting the 2n pollen; (6) performing sexual polyploidy hybridization; and (7) identifying sexual triploid descendants. By the method, the sexual triploids of the colored zantedeschia can be successfully obtained in one step.

The invention discloses a strain YAB-3 for degrading PFOA (perfluorooctanoic acid) and an application thereof. The strain for degrading PFOA is sphingomonas yanoikuyae, with the collection number of CCTCC (China Center for Type Culture Collection) NO:M2015281, the collection date of May 7, 2015 and the collection unit of CCTCC. A method for degrading PFOA-containing materials by utilizing the strain YAB-3 is characterized by comprising the following steps: firstly inoculating the single colony YAB-3 into an LB culture medium for 12-18 hours, then preparing bacterial suspension with certain OD600 with the culture medium, and finally treating the PFOA-containing materials with the bacterial suspension. The invention provides the strain YAB-3 for degrading PFOA and the method for treating the PFOA-containing materials by utilizing the strain. The strain has strong capability of degrading PFOA. The method for treating the PFOA-containing materials is simple and effective and can achieve the effect of obviously reducing the concentration of PFOA in the materials.

Provided is a method for screening a phosphorous high efficiency utilizing variety from multiple barley varieties. The method comprises the steps of selecting barley varieties with the same sizes of grains, putting the varieties into a culture dish, and germinating the varieties into seedlings with roots; selecting seedlings of which growth is consistent from the seedlings, dividing the seedling of which growth is consistent into two groups, and using a nutrient solution with different phosphorus concentration to pour on the seedlings until the barley is mature; measuring and calculating the relative plant height, the relative kernels per spike and the relative thousand seed weight of each variety, conducting comprehensive comparison, and selecting several barley varieties of which the three indexes are comprehensively the highest as phosphorus high efficiency utilizing varieties; germinating the barley varieties into after-screening seedlings with roots, selecting the after-screening seedlings of which the growth is consistent, dividing the after-screening seedlings into two groups, using the nutrient solution with different phosphorus concentration to conduct water planting, and measuring and calculating the relative dry matter weight, the relative phosphorus content and the relative chlorophyll content of each variety; comprehensively comparing, and selecting one barley variety of which the three indexes are comprehensively the highest as the variety of which the phosphorus utilizing efficiency is relatively the highest. According to the method for screening the phosphorous high efficiency utilizing variety from multiple barley varieties, the finally screened barley variety provides materials for further improving an existing variety and studying the biological characteristics of the existing variety and a physiology and biochemistry mechanism.

The invention discloses a cyclocarya paliurus non-tube rapid propagation method. According to the cyclocarya paliurus non-tube rapid propagation method, plant tissue culturing principles are adopted, pruning and sprouting promotion of seedlings of cyclocarya paliurus maternal plant plantations are carried out, light matrix non-woven fabric nutritional bags are used, rooting hormone is applied, bactericidal treatment is carried out, all sunlight intelligent breeding greenhouses are adopted, batch-type mist spray is controlled accurately, so that seedling period of cyclocarya paliurus is shortened, seedling surviving rate and yield of cyclocarya paliurus are increased, problems that formation of callus tissue is inhibited because cyclocarya paliurus is high in tannin content, and cottage processing ratio is low are solved, and a problem that cyclocarya paliurus genetic resources are scarce is solved effectively.

The invention provides a method for protecting and restoring germplasm of citrus-Hunglongbing-infected citrus according to a two-step method. The method comprises the steps of soaking seeds into alcohol with concentration of 70% for one minute, soaking the seeds into NaOH with concentration of 5% for eliminating pectin, then soaking the seeds into sodium hypochlorite solution with concentration of 3% for 20 minutes, cleaning with sterile water by 2-3 times, then immersing the seeds in normal-temperature water for 5-10 hours, then performing thermal treatment on the seeds by means of wet-and-hot air with temperature of 50-55 DEG C for 40-60 minutes, then removing seed shells, sowing the seeds into an MS medium in which enrofloxacin sodium and sulfadimethoxine sodium antibiotic are added, transplanting seedlings into disinfected nutrient soil after the seedlings are grown to a height of 5cm, and grafting the newly grown branch from a nursery stock as a scion on a stock seedling which is grown from a stock seedling which is processed in the same way. According to the method of the invention, the antibiotic composition which comprises the enrofloxacin sodium and the sulfadimethoxine sodium antibiotic is utilized for reasonable mixing; and a two-step method which comprises thermal treatment and adding the antibiotic is firstly utilized for performing sterilization on the citrus-Hunglongbing-infected citrus, thereby realizing higher sterilization effect.

The invention discloses a breeding method of a high-temperature-resistant stichopus japonicus strain and belongs to the field of genetic breeding of aquatic products. Stichopus japonicus surviving after natural high temperature elimination in summer in different regions is selected as a parent, families are constructed through breeding after sexual maturity, heat shock at the temperature of 28 DEG C is performed at the auricularia stage of each family, 20% of families are reserved according to the survival rate and the malformation rate, the reserved families are mixed and bred until the average weight of individuals is larger than 50 g, high-temperature stress is performed at 32 DEG C to remove dead and damaged individuals, less than 25% of individuals are reserved and continuously bred until sexual maturity, heat shock selection and breeding of three generations are performed continuously in the same principle, and the new stichopus japonicus strain with the high-temperature-resistant character is obtained. The original parent of stichopus japonicus better conforms to the tolerance characteristic of the actual breeding environment; high-temperature domestication screening of the families in the larva period and high-temperature stress screening in the breeding period are performed, family breeding and group breeding are combined, and the selection efficiency is improved; breeding of three generations is performed continuously, the high-temperature-resistant character is stabilized, and the breeding method has good economic benefit and broad breeding prospect.

The invention discloses brassica napus L promoter pBnUnng0942890 and application thereof. By cloning a promoter of a gene pBnUnng0942890 in the brassica napus L, a plant expression vector of a reporter gene GUS regulated by the promoter is constructed; by using an agrobacterium-mediated inflorescence impregnating method to transform arabidopsis, GUS histochemical staining is carried out on screened positive transgenic strains; the result shows that the pBnUnng0942890 has the functions of driving preferential expression of downstream genes in roots, flowers and seeds, and not expressing or realizing low expression in other tissues and organs. The promoter has good application potential in the aspects of improving crop quality by rapeseed transgene, artificially creating germplasm resourcesand the like.

The invention belongs to the field of sorghum breeding, and particularly relates to a sorghum dwarfing mutation breeding method. The method comprises the steps of conducting pretreatment after seed selection; conducting physical mutation, wherein sorghum seeds are put into a plastic bag for irradiation treatment; conducting chemical mutation, wherein the seeds subjected to irradiation treatment are soaked in a chemical mutation agent for treatment, and chemical mutation seeds are obtained; conducting mutation aftertreatment; screening dwarfing sorghum mutants; planting M1-generation seeds harvested from a single plant in the next year to form M2-generation strains, selecting untreated seeds as a contrast, comparing the flowering period with the contrast, observing the stability of the characters of the flowering period in the strains, conducting mixed harvesting and mixed threshing on the seeds which tend to be stable, harvesting M3-generation seeds, and obtaining a dwarf variant sorghum variety; and planting the strains with the characters continuously separated, continuing to plant and harvest the single plant, harvesting M3-generation seeds, and continuing to conduct identification and selection on the next system in the M4 generation till the dwarf variant sorghum variety is obtained.

The invention discloses a molecular marker AhyWptc closely linked with white seed coats of peanuts and application of the molecular marker AhyWptc, and relates to the technical field of agricultural biology. A specific primer pair of the molecular marker AhyWptc comprises a forward primer as shown in SEQ ID NO: 1 and a reverse primer as shown in SEQ ID NO: 2. By utilizing the molecular marker AhyWptc provided by the invention, the seed coat color of a next-generation harvested material can be determined in advance by detecting the DNA of a cotyledon, and the molecular marker AhyWptc is applied to molecular breeding and peanut variety improvement, so that the breeding time can be greatly shortened, the breeding efficiency can be improved, and more excellent peanut germplasm resources can be obtained within shorter time.

The invention provides a method for acquiring asparagus homozygous diploid by culturing anther. The method comprises the following steps: acquiring asparagus anther, pre-treating the asparagus anther,performing callus induction for the asparagus anther, performing sub-generation differentiation for the asparagus anther, rooting a test-tube seedling, and multiplying haploid chromosome. By adoptingthe method for acquiring the asparagus homozygous diploid by culturing anther, the induction rate of callus with hard and compact texture of the asparagus anther is 7.2 to 17 percent; and the callusinduction rate of the variety ''champion'' with hard and compact texture is 15.8 to 17 percent or higher. According to the method for acquiring asparagus homozygous diploid by culturing anther, the haploid differentiation rate is 2.2 to 7.2 percent, the success rate for obtaining the homozygous diploid and multiploid by inducing the haploid is 45.86 to 49.73 percent, and the ratio of the homozygous diploid is 1.6 to 2.9 percent.

The invention provides Raoultella sp. and application thereof in pyrene-Cr(VI) composite pollution co-detoxication in a tolerant environment. Concretely, the Raoultella sp. has the effect of degradingpyrene and reducing Cr(VI) in the tolerant environment.