64results about How to "Increased risk of contamination" patented technology

The present invention provides a cell culture data and cell image capturing and remote monitoring system that includes an imaging sensor pod having one or more cameras to capture multiple color still and / or motion images of cell cultures and cells located on a support and / or in a liquid located in a culture vessel within an incubator. The images can be taken at varying magnifications, and from different discrete locations on the support or in the vessel round the clock. The captured data and images are wirelessly transmitted to a management control unit, and / or another wirelessly connected and remotely located data transmission device such as PDA, for a researcher to review and analyze to determine the health and viability of cells, and the status of the cell culture. The image capturing and remote monitoring system taught herein alleviates the need for a researcher to be in the physical presence of a biological sample in order to visually ascertain and analyze the health and viability of the sample and status of the cell culture.

A monolithic sputtering target assembly having a one piece assembly made from the same material is disclosed. Also disclosed are other sputtering target assemblies which have a backing plate and a sputtering target blank wherein the backing plate is made from or contains a metal, such as a valve metal, cobalt, titanium, or alloys thereof. Methods of recycling target assemblies are further disclosed as well as unique methods of providing target assemblies to fabricators.

A fluidized bed process for the production of polycrystalline silicon granules supplies, in addition to reaction gas, a gas containing 99.5 to 95 mol. percent hydrogen and 0.5 to 5 mol. percent gaseous silicon compounds, and the reactor wall is maintained at the same or a higher temperature than the reaction zone, such that the deposition of silicon on reactor internals is minimized.

A disposable face mask that changes color as an indicator of fever to provide hospitals with a quick and inexpensive method for triaging infected patients while limiting exposure to others.

Influenza viruses for use in preparing human vaccines have traditionally been grown on embryonated hen eggs, although more modern techniques grow the virus in mammalian cell culture e.g. on Vero, MDCK or PER.C6 cell lines. The inventor has realised that the conditions used for influenza virus culture can increase the risk that pathogens other than influenza virus may grow in the cell lines and have identified specific contamination risks. Suitable tests can thus be performed during manufacture in order to ensure safety and avoid iatrogenic infections.

The invention discloses a method for jointly stabilizing fly ash and an arsenic-containing waste and relates to a treatment process for jointly stabilizing two hazardous wastes. The method comprises the following steps of: sequentially adding the fly ash and the arsenic-containing waste into a kettle type reactor according to a dry basis mass ratio of 1:(20-40); then adding a regulator solution being 10-40 percent of the weight of solid; and mechanically stirring and reacting for 5-30 minutes at normal temperature. After reacting, the volume of the mixed wastes is reduced by 10-30 percent, and the stabilization features of the product are greatly improved. Arsenic in the arsenic-containing waste is stabilized by the utilizing the physical and chemical characteristics of small grain diameter, large specific surface area, high Ca content, strong alkalinity, gel activity, and the like of the fly ash, and meanwhile, the migratory capacities of heavy metals such as Pb, Se, Cd, Ni, and the like in the fly ash are also obviously reduced and conform to an arrival standard of a hazardous waste landfill site. The method treats wastes by using wastes, greatly reduces the treatment cost, has simple equipment and considerable economic, social and environmental benefits and can be widely popularized and applied to the treatment of the fly ash and the arsenic-containing waste so as to achieve the purpose of stabilization.

The invention relates to the field of medical instrument in-vitro diagnostic reagents, in particular to a cleaning solution or diluent thereof special for a full-automatic chemiluminescence tester anda preparation method thereof. The cleaning solution comprises an inorganic salt, a phosphate buffer system, a surfactant 1, a surfactant 2, a preservative and water; the inorganic salt consists of sodium chloride and potassium chloride; the phosphate buffer system is one of a disodium hydrogen phosphate-potassium dihydrogen phosphate system and sodium dihydrogen phosphate-dipotassium hydrogen phosphate; the surfactant 1 is one of tween-20 and tween-80; the surfactant 2 is one of sodium dodecyl sulfate and sodium laurate; and the preservative is composed of Proclin300 and Bronidox. The cleaning solution provided by the invention has the characteristics of simple preparation process and low cost, is suitable for full-automatic chemiluminescence testers of different manufacturers, at the same time is suitable for detection of items with different detection principles, and has good accuracy, high precision and good corrosion resistance.

The present invention relates to a ventilating device for providing a zone (1) of clean air between the ventilating device and a workplace region (2), which ventilating device comprises air supply units (3) adapted to generating laminar air flows intended to constitute said clean air zone. The ventilating device comprises at least three air supply units (3) disposed in a closed pattern and air stop and guide units (4) which prevent or hinder air with bacteria-bearing and other pollutant particles which surrounds the clean air zone (1) from being drawn in between the air supply units and into the clean air zone. The air stop and guide units (4) are also configured to guide parts of the air flows from adjoining air supply units (3) outwards from the centre of the clean air zone instead of towards the air supply units, and other parts of the air flows from adjoining air supply units in towards the centre of the clean air zone instead of towards the air supply units, thereby minimizing the increased downward velocity which occurs when the air flows from mutually adjacent air supply units meet in an uncontrolled manner.

A monolithic sputtering target assembly having a one piece assembly made from the same material is disclosed. Also disclosed are other sputtering target assemblies which have a backing plate and a sputtering target blank wherein the backing plate is made from or contains a metal, such as a valve metal, cobalt, titanium, or alloys thereof. Methods of recycling target assemblies are further disclosed as well as unique methods of providing target assemblies to fabricators.

A method of cleaning a vessel having deposits on an interior surface includes removably bonding an ultrasonic transducer to an external wall of the vessel and using the ultrasonic transducer to produce ultrasonic energy coupled into the vessel wall such that at least a portion of the ultrasonic energy is transmitted to the interior surface.

Method of manufacturing a microscale nozzle, comprising the steps of forming a microscale channel in the top surface of a substrate, said microscale channel comprising an inlet end and a nozzle-end, depositing a nozzle-forming layer in a section of the microscale channel, and removing material from the substrate at the nozzle-end of the microscale channel to expose at least a portion of said nozzle-forming layer. The manufactured microscale nozzle may be used for transferring a liquid sample form a microchip fluidic system into an external analytical device.

The invention discloses a mmethod for obtaining analogous retinal tissues rich in cone and rod cells by using human induced pluripotent stem cells. The method comprises the following steps: digestinghiPSCs to obtain cell pellets, and then carrying out suspension cultivation on the cell pellets to obtain an embryoid body; reinoculating the embryoid body into a culture dish enveloped by Matrigel, and carrying out induced differentiation inside an induced cultivation solution to obtain nerve retina (NR) and RPE; stirring up the NR and the RPE, carrying out suspension cultivation to obtain 3D analogous retina comprising NR tissues, then carrying out continuous suspension cultivation inside an optimized culture solution without retinoic acid, carrying out NR differentiation on all retinal cells, comprising highly matured photoreceptor cells, Rhodopsin positive rod cells, L / M OPSIN positive red and green cone cells and S-OPSIN positive blue cone cells, and thus particularly obtaining the analogous retinal tissues rich in red and green cone and rod cells.

A mirror for reflecting extreme ultraviolet light (EUV) comprising: a substrate layer; and an upper layer above the substrate layer, that reflects EUV wavelengths and refracts longer wavelengths, said upper layer being dense and hard carbon having an Sp2 to Sp3 carbon bond ratio of 0 to about 3 and a normal incidence EUV mirror comprising an optical coating on an uppermost surface which permits transmission of EUV and protects the surface from environmental degradation, said coating being dense and hard and having an Sp2 carbon bond ratio of 0 to about 3 and a thickness of 0.1 to about 5 nanometers. The invention also includes EUV mirror systems protected by a dense carbon layer and includes a multilayer EUV reflecting system having an out of band absorbing layer.

A composition containing a macrophage inhibitor may be administered in an effective amount to prevent, inhibit or reduce restenosis, thrombus or aneurysm formation in implanted polymeric vascular grafts. The composition may be administered prior to vascular graft implantation, at the same time as vascular graft implantation, following vascular graft implantation, or any combination thereof. Examplary macrophage inhibitors include bisphosphonates, anti-folate drugs and antibodies, preferably in a controlled release or liposomal formulation.

One embodiment of the invention is directed to a genetically enhanced cyanobacterium for the production of a first chemical compound, comprising at least one first recombinant gene encoding a first biocatalyst for the production of the first chemical compound, wherein the gene is under the transcriptional control of a Co2+ or Zn2+-inducible promoter. Such a cyanobacterium can provide a tighter control of the production of the first chemical compound.

The invention relates to a cell culture medium with the anti-pollution function, and belongs to the field of biotechnology. The cell culture medium disclosed by the invention aims to solve the problem that the current cell culture medium, especially a fluid culture medium cannot prevent contamination. In order to solve the problem, a method of adding 10-20 mg of ethyl ciprofloxacin to per liter of the culture medium is adopted, so that the newly prepared fluid culture medium has continuous anti-pollution capacity.

The present invention relates to a ventilating device for providing a zone (1) of clean air between the ventilating device and a workplace region (2), which ventilating device comprises air supply units (3) adapted to generating laminar air flows intended to constitute said clean air zone. The ventilating device comprises at least three air supply units (3) disposed in a closed pattern and air stop and guide units (4) which prevent or hinder air with bacteria-bearing and other pollutant particles which surrounds the clean air zone (1) from being drawn in between the air supply units and into the clean air zone. The air stop and guide units (4) are also configured to guide parts of the air flows from adjoining air supply units (3) outwards from the centre of the clean air zone instead of towards the air supply units, and other parts of the air flows from adjoining air supply units in towards the centre of the clean air zone instead of towards the air supply units, thereby minimizing the increased downward velocity which occurs when the air flows from mutually adjacent air supply units meet in an uncontrolled manner.

The invention relates to a method for tendering buffalo. The method comprises the following steps of: uniformly injecting exogenous enzyme liquid into buffalo according to an injection enzyme using amount of 10-80 U in 1g of buffalo and an injection volume of 0.01-0.08 mL in 1g of buffalo; standing for 3 minutes; rolling for 2 minutes; after leaching or wiping the enzyme liquid discharged to the surface, hermetically packaging with food plastic packaging bags made of polyethylene or polyvinylidene chloride; tendering for 6-8 hours under the environment of 6-12 DEG C; after tendering is finished, cooking and processing directly or storing under the condition of 0-4 DEG C for standby within 24-48 hours. According to the method, the tendering efficiency is high, the effect is obvious, and the shearing force of buffalo after being tendered is reduced by more than 45%. According to the method, influence on appearance of buffalo and product yield is slight, the freshness of the buffalo is well kept, safety and non-toxicity without bad flavor can be realized, the industrialized operability is high, and the method is suitable for tendering treatment of buffalo for cooking or deep processing.

The invention discloses a carbon emission data verification method of a single building, and relates to the technical field of carbon emission data verification. The method comprises the steps: acquiring the fuel purchase interval duration and the product gas type number of each single building, dividing the monitoring time into a plurality of time periods, collecting the greenhouse gas emission amount of each single building in each time period, and judging whether the emission amount exceeds the standard or not and the standard exceeding time period; if the number of the corresponding single buildings in the same standard exceeding time period exceeds the standard, adjusting the single buildings in the corresponding standard exceeding time period in batches; otherwise, monitoring the single building in the corresponding standard exceeding time period; collecting the peripheral vegetation area of each single building, the average daily traffic flow of the peripheral road and the treatment duration of the corresponding discharged gas, and acquiring the gas influence coefficient of each single building; if the gas influence coefficient exceeds the standard, performing carbon emission data verification on the corresponding building; and otherwise, counting the carbon emission data of the corresponding building.

Methods for increasing the patency of biodegradable, synthetic vascular grafts are provided. The methods include administering one or more cytokines and / or chemokines that promote outward tissue remodeling of the vascular grafts and vascular neotissue formation. The disclosed methods do not require cell seeding of the vascular grafts, thus avoiding many problems associated with cell seeding. Biodegradable, polymeric vascular grafts which provide controlled release of cytokines and / or chemokines at the site of vascular graft implantation are also provided.

Methods for treating or preventing neointima stenosis are disclosed. The methods generally involve the use of a TGFβ inhibitor, a SMAD2 inhibitor, an FGF Receptor agonist, a Let-7 agonist, or a combination thereof, to inhibit endothelial-to-mesenchymal transition (Endo-MT) of vascular endothelial cells into smooth muscle cells (SMC) at sites of endothelial damage. The disclosed methods can therefore be used to prevent or inhibit neointimal stenosis or restenosis, e.g., after angioplasty, vascular graft, or stent. Also disclosed are methods for increasing the patency of biodegradable, synthetic vascular grafts using a composition that inhibits Endo-MT. A cell-free tissue engineered vascular graft (TEVG) produced by this method is also disclosed.

The invention relates to an environment-friendly silver-loaded antibacterial agent based on plant waste powder and a preparation method of the environment-friendly silver-loaded antibacterial agent. The preparation method comprises the following steps of adding the plant waste powder, silver salt and a silane coupling agent according to a certain ratio, performing uniform mixing, and controlling the reaction temperature and time to obtain the environment-friendly silver-loaded antibacterial agent. Silver ions are uniformly adsorbed under the action of porous structures by utilizing reducing groups and the porous structures which are rich in plant wastes, nano-silver grows through in-situ reduction under the action of the reducing groups, and meanwhile, the nano-silver is uniformly anchoredinside and on the surfaces of plant tissues by virtue of the porous structures, so that preparation of the silver-loaded antibacterial agent is realized in one step in a drying process of the plant waste powder. The preparation method solves the problems of high cost, easy agglomeration, easy dissolution of silver ions and weak interaction with a high polymer material for the nano-silver; the plant waste powder is taken as a substrate, is environment-friendly, easy to obtain and low in cost, and conforms to the concept of sustainable development; the preparation process is simple; and extra use of special equipment and reagent, especially a reducing agent is avoided.

The invention relates to packaging (10) for solid yeast products, such as: a supporting element (12); solid yeast products contained in one or more packages (22), supported by the supporting element (12); and an outer cover (14) retaining all of the solid yeast products contained in the packages (22) on the supporting element (12). According to the invention, all of the packages (22) located between the solid yeast products and the outer cover (14) may have a surface density no greater than 200 g / m2. The invention also relates to a method for packaging solid yeast products and to a method for preserving and using solid yeast products thus packaged.

The invention relates to a CRISPR-Cas system, in particular to a CRISPR-Cas system for diagnosing spinal muscular atrophy and application thereof. The CRISPR-Cas system for diagnosing spinal muscularatrophy disclosed by the invention comprises Cas14a1, crRNA, ssDNA and FQ-probe. A method based on combination of CRISPR / Cas14a1 and asymmetric PCR constructed by the invention can perform gene detection for SMA patients, and can be used for specifically detecting the mutation condition of SMN1 gene. Different from an existing CRISPR / Cas12a SMA diagnosis technology, the kit can also distinguish SMN2 gene interference so as to judge whether a detected person suffers from the spinal muscular atrophy or not, and has the characteristics of simple and convenient operation, high specificity and lowcost.

A container (300) adapted to store a quantity of metal powder (101, 301), the container (300) comprising a container body having an opening and a sensing device (111, 121) for sensing the temperature of the powder (101, 301) and / or the humidity level inside the container (300), wherein a first reading received from the sensing device (111, 121) is compared to a second reading received from a second sensing device configured to sense the temperature and / or humidity level of an environment outside of the container, and based on the first reading and the second reading, a user is provided within an indication about whether the container can be opened.

The invention provides fully-enclosed flow-stabilized and pressure-superposed energy storage water supply equipment. According to the fully-enclosed flow-stabilized and pressure-superposed energy storage water supply equipment, the problem that a common pressure-superposed water supply product has no water storage capacity or has extremely small water storage capacity is solved, and dual functionsof water storage and energy storage are introduced into a pressure-superposed water supply system; the water contention degree of a plurality of secondary water supply stations to a municipal primarywater supply pipe network is greatly reduced, the reactive power loss of water supply is greatly eliminated, the water supply efficiency is improved, and continuous, safe and high-quality water supply for users can still be realized under the condition that the flow quantity and pressure of a municipal tap water pipe network cannot meet user requirements continuously; even under the condition that the water supply of the municipal tap water pipe network is completely cut off, the water supply still can continue for a number of minutes to a number of hours; positive pressure full enclosing ofwater storage facilities can be realized, the purposes of chlorine maintaining, self cleaning and no cleaning are achieved, and the fully-enclosed sanitary safety requirement in the Chinese water supply standard is fully met; and the blank that no secondary water supply products of other forms exists between pressure-superposed water supply and water tank (pool) water storage water supply in the secondary water supply products for many years is filled up.

One embodiment of the invention is directed to a genetically enhanced cyanobacterium for the production of a first chemical compound, comprising at least one first recombinant gene encoding a first biocatalyst for the production of the first chemical compound, wherein the gene is under the transcriptional control of a Co2+ or Zn2+-inducible promoter. Such a cyanobacterium can provide a tighter control of the production of the first chemical compound.

The invention discloses a novel PCV (porcine circovirus) mutant strain and a porcine kidney cell strain co-culturing with the novel PCV mutant strain. Cells infected by the novel PCV mutant strain have visible minimal lesions, but cell death is not caused. The sieved porcine kidney cell strain carries a PCV2, so that stable passage can be realized, a large number of viruses are generated, and the prepared viruses can be used for preventing and treating the PCV.