The invention discloses a large-scale production method for veterinary
pseudorabies attenuated live vaccines. The method comprises the steps of culturing
pseudorabies virus attenuated strains by using a
cell suspension process so that the density of BHK21-C13 cells subjected to
suspension culture in a reactor is more than or equal to 2*10<6> cells per milliliter, then culturing with a
virus maintenance solution containing
pseudorabies suspended
virus seeds, and performing clarification,
seedling, packaging and freeze-
drying on the harvested pseudorabies attenuated virus solution to obtain the veterinary pseudorabies attenuated live vaccines. By adopting the method,
mass multiplication of pseudorabies attenuated viruses can be realized, the
titer of viruses and the yield of
antigen are greatly improved, process
automation is realized, the quality of the vaccines is improved, the density of the BHK21-C13 cells can be improved, the optimal biochemical condition of
cell growth or
virus multiplication can be automatically monitored, the
titer of viruses is improved, large-scale production of the process is relatively easy, and the vaccines are stable in quality and low in batch difference and have broad production and application prospect.