Method for producing PRRS (Porcine Reproductive and Respiratory Syndrome) viruses
A production method and a syndrome technology are applied in the production field of porcine respiratory and reproductive disorder syndrome virus, which can solve the problems of contaminating vaccine quality, being easily polluted by the environment, high labor intensity, etc., achieving uniform product quality, reducing production costs, The effect of a high degree of automation
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[0029] The present invention will be further described below in conjunction with the accompanying drawings and specific embodiments.
[0030] (1) Select a torrent-perfusion bioreactor as the means of cultivation: 10L torrent-perfusion bioreactor.
[0031] (2) Select the paper carrier as the carrier for cell attachment and growth: polyester amide paper carrier.
[0032] (3) Pretreatment method of the paper carrier: 1) soak the paper carrier with sterile PBS overnight; 2) wash the paper carrier 3 times with sterile PBS; 3) soak the paper carrier with cell culture growth solution for 2 hours above.
[0033] (4) Marc-145 cells were selected as cells for seedling production.
[0034] (5) Subculture and cultivation of cells for seedling production.
[0035] The above-mentioned cells were digested and passaged with trypsinized solution, and continued to be cultured with a cell growth medium containing 10% bovine serum, 1% double antibody (mixed solution of penicillin and streptomy...
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