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Preparation method of swine pseudorabies vaccine

A technology for porcine pseudorabies and inactivated vaccine, which is applied in the field of vaccine preparation, can solve the problems of inability to prevent virulent replication, excretion, formation of latent infection, and strong virulence, and achieves reduced cost burden, low price, and immunogenicity. Good results

Inactive Publication Date: 2011-03-30
SHANGHAI ELITE AGRI SCI TECH GROUP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The attenuated virus vaccine has good immunogenicity. Although it can prevent the emergence of clinical symptoms after vaccination, it cannot prevent the virulent virus from replicating, excreting and forming latent infection in the infected animals. Whether it can cause the virulence to become stronger remains to be further studied.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A preparation method of porcine pseudorabies inactivated vaccine, the method may further comprise the steps:

[0030] (1) pig testis (ST) cells are placed in the growth solution of newborn bovine serum MEM with a concentration of 8% by weight to prepare ST cells, and receive poison when the cells are full of monolayers;

[0031] (2) isolate porcine pseudorabies virus (PRV-sh strain) from sick pigs suffering from porcine pseudorabies, and its virulence TCID 50 ≥10 8.0 / ml, the virus strain is diluted to a concentration of 0.1% by weight, inoculated into the well-growing porcine testis cell monolayer obtained in step (1), at 37°C, the porcine testis cells are controlled to adsorb porcine pseudorabies virus for 1h, and then The cell maintenance solution placed in neonatal calf serum MEM with a concentration of 2% by weight is controlled at a temperature of 37° C. for static culture;

[0032] (3) Observing twice a day, recording the cell lesions, when more than 80% of the...

Embodiment 2

[0051] A preparation method of porcine pseudorabies inactivated vaccine, the method may further comprise the steps:

[0052] (1) Pig testis (ST) cells are placed in the growth solution of newborn bovine serum MEM with a concentration of 5% by weight to prepare ST cells, and receive poison when the cells are full of monolayers;

[0053] (2) isolate porcine pseudorabies virus (PRV-sh strain) from sick pigs suffering from porcine pseudorabies, and its virulence TCID 50 ≥10 8.0 / ml, the virus strain is diluted to a concentration of 0.1% by weight, inoculated into the well-growing porcine testis cell monolayer obtained in step (1), at 37°C, the porcine testis cells are controlled to adsorb porcine pseudorabies virus for 1h, and then The cell maintenance solution placed in neonatal bovine serum MEM with a concentration of 2% by weight is controlled at a temperature of 37°C for rotary culture;

[0054] (3) Observing twice a day, recording the cell lesions, when more than 80% of the...

Embodiment 3

[0059] A preparation method of porcine pseudorabies inactivated vaccine, the method may further comprise the steps:

[0060] (1) pig testis (ST) cells are placed in the growth solution of newborn calf serum MEM with a concentration of 5% by weight to prepare ST cells, and receive poison when the cells are full of monolayers;

[0061] (2) isolate porcine pseudorabies virus (PRV-sh strain) from sick pigs suffering from porcine pseudorabies, and its virulence TCID 50 ≥10 8.0 / ml, the virus strain is diluted to a concentration of 0.05% by weight, inoculated into the well-growth pig testicular cell monolayer obtained in step (1), at 35° C. to control the adsorption of porcine pseudorabies virus by the pig testicular cells for 0.5h, and then This is placed in the cell maintenance solution of newborn bovine serum MEM with a concentration of 0.5% by weight, and the temperature is controlled to be 35° C. for static culture;

[0062] (3) Observing twice a day, recording the cell lesio...

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Abstract

The invention relates to a preparation method of a swine pseudorabies vaccine, which comprises the following steps of: culturing a virus by using a swine testicle cell, and when one layer of cells grows, inoculating a swine pseudorabies virus; then adding into a cell maintenance medium, statically or rotatably culturing, when the cell suffers from more than 80 percent of pathological changes, harvesting a cell culture, repeatedly freeze-thawing to obtain a cell venom containing supernate, and mixing the cell venom qualified in toxic valence detection with formaldehyde for inactivating; and mixing with an emulsifying agent for emulsifying to obtain the swine pseudorabies vaccine. Compared with the prior art, a strain used for preparing the vaccine has the advantages of stronger toxicity and high virus valence; the swine pseudorabies vaccine has good immunogenicity and long immunization period; and the preparation method has the advantages of reasonable process and lower cost, thereby greatly lowering the cost load of the fish breeding and poultry raising industry.

Description

technical field [0001] The invention relates to a preparation method of a vaccine, in particular to a preparation method of an inactivated porcine pseudorabies vaccine. Background technique [0002] Pseudorabies (PR) is an infectious disease that occurs in domestic animals and wild mammals with fever, itching (except pigs) and encephalomyelitis as the main symptoms. The disease is a typical natural foci disease, pigs are its main host and source of infection, and the economic losses caused to the pig industry are second only to swine fever. In other animals except pigs, the disease mostly occurs in a sporadic form, and after the disease occurs, it all ends in death. [0003] Due to the loss caused by pseudorabies is second only to foot-and-mouth disease and swine fever, it has become one of the swine diseases that some European countries focus on epidemic prevention. PR distribution is worldwide. In the middle of the 20th century, PR was widespread in Eastern Europe and the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/245A61P31/20
Inventor 彭丽英倪建平李春华邹勇张婉华蒋凤英朱永军赵本进苏万国陈晓清
Owner SHANGHAI ELITE AGRI SCI TECH GROUP
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