Production method of porcine reproductive and respiratory syndrome virus

Abstract

The invention discloses a production process of a porcine reproductive and respiratory syndrome virus (PRRSV) by using a bioreactor to culture a Marc-145 cell, which comprises the following technical steps of: (1) selecting a Marc-145 cell as a vaccine preparation cell; (2) culturing the vaccine preparation cell through passage and amplification; (3) reproducing seed virus for production; (4) culturing the microcarrier of the Marc-145 cell in the bioreactor; (5) producing an antigen of the PRRSV; and (6) treating the harvested virus antigen liquid. The invention can greatly reduce production cost and increase the input-output ratio by 5-10 times. Moreover, the invention has the advantages of short production period, small occupied land, easy and rapid expansion of production scale, less environment pollution, easy treatment, high automation degree, fewer people and easy realization of balanced and stable quality. The production cost can be obviously increased, and the yield and the quality of the vaccine can be increased.

Description

technical field [0001] The invention relates to a method for producing vaccines using bioreactor microcarrier cell culture technology, which can be used for industrial production of porcine respiratory and reproductive disorder syndrome vaccines and replaces the traditional production process of rotary bottle culture method. Background technique [0002] At present, the production of porcine respiratory and reproductive disorder syndrome vaccine in China mainly relies on the "cell spinner bottle culture method". The traditional process is labor-intensive, time-consuming, low in efficiency, and high in production cost; it is easy to be polluted by the environment; there are large differences between different production batches or between different bottles of the same production batch; it is difficult to expand production; it is easy to be contaminated by bacteria or other viruses The hidden dangers of vaccine quality caused by this problem involve biosafety and public health...

AI Technical Summary

Problems solved by technology

The traditional process is labor-intensive, time-consuming, low in efficiency, and high in production cost; it is easy to be polluted by the environment; there are large differences between different production batches or between different bottles of the same production batch; it is difficult to expand production; it is easy to be contaminated by bacteria or other viruses The hidden dangers of vaccine quality, involving biosafety and public health issues

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