40results about How to "High poison price" patented technology

The invention discloses a method for preparing a classical swine fever (CSF) vaccine by using a cell microcarrier suspension culture system, which comprises the following steps of: (1) inoculating cells for preparing the vaccine to a carrier tank containing culture solution and a microcarrier, and uniformly mixing the cells and the microcarrier to make the cells attached to the microcarrier; (2) when the concentration after cell proliferation is 5 to 40 times of the initial inoculation concentration, inoculating CSF virus (lapinized virus) to the cells according to multiplicity of infection (M.O.I.) of the virus of 0.01-1 and reproducing the virus; and (3) mixing prepared virus liquid, adding an appropriate freeze-drying protective agent, fully and uniformly mixing, quantitatively packaging, and freeze-drying to obtain the CSF vaccine. The CSF vaccine produced by the method has the advantages of high density of cultured cells, continuous culture, high yield of the virus, high immune effect, high safety, complete immune protection on attack of violent CSF, and the like.

The invention discloses a method for preparing virus of porcine reproductive and respiratory syndrome on a large scale. In the method, the virus of the porcine reproductive and respiratory syndrome is prepared in a cell microcarrier suspension culture system by a bioreactor. The method comprises the following steps of: inoculating host cells for preparing the virus to a carrier tank containing culture solution and a microcarrier, and mixing the cells and the microcarrier uniformly to ensure that the cells are attached to the microcarrier; providing sufficient nutrients and appropriate gas environment for the cells under the appropriate culture environment to ensure that the cells are grown until the cells are in an amount which are 10 to 20 times of the inoculation concentration on the microcarrier; preparing virus suspension from the virus of the porcine reproductive and respiratory syndrome by using cell maintenance culture solution to ensure that the suspension is adsorbed to the cells; culturing the virus under the appropriate culture environment; culturing continuously for 2 to 3 days to obtain virus solution; and after the virus solution passes inspection, performing freeze thawing on the virus solution twice at the temperature of -20 DEG C, and inactivating and purifying to prepare an inactivated vaccine of the porcine reproductive and respiratory syndrome or adding a freeze-drying protective agent for freeze drying to prepare a live vaccine of the porcine reproductive and respiratory syndrome. The method has large production scale, high yield of single batch and low production cost.

The invention relates to a production method of hog cholera live vaccine, comprising the following technical steps: (1) cloning and identifying a hog cholera lapinized low virulent strain; (2) breeding cloned strain seed virus; (3) breeding cells for production; (4) breeding virus liquid for production; and (5) matching vaccine, carrying out split charging and freeze-drying. The method provided by the invention has the characteristics of simple and stable production technology, good virus immunogenicity and genetic stability, small risk of exogenous virus pollution, low production cost and the like, and is convenient for inspection. The hog cholera live vaccine produced by the production method has the advantages of good genetic stability, high purity and strong immunogenicity.

The invention discloses a method for preparing porcine circovirus through basket reactor fermentation. The method for preparing the porcine circovirus through the basket reactor fermentation is characterized by comprising the following steps: (1), preparing the host cell species; (2), preparing a basket reactor before inoculation; (3), performing host cell propagating culture; and (4), performing virus proliferating culture: emptying cell growing liquid in the reactor, supplementing cell maintaining liquid with the volume equal to that of the emptied cell growing liquid, inoculating the porcine circovirus, 36-48h after inoculation, harvesting a virus solution for the first time, after harvesting the virus solution, adding the cell maintaining liquid with the volume equal to that of the harvested virus solution into the reactor, propagating, reproducing, and harvesting the virus again, circularly repeating for multiple times until the sugar consumption of cells in the virus solution to be harvested is detected to be reduced down to 0.5g / L / day, and harvesting the last porcine circovirus solution in the batch. The method is simple and feasible; and by the method, the production cost can be greatly reduced, the unit titer of a porcine circovirus antigen is significantly improved, and the harvest of the virus solution antigen in each fermentation batch is increased.

The invention relates to the field of veterinary medicine, in particular to an attenuated strain of canine parainfluenza virus, an application thereof and a vaccine. The present invention proposes an attenuated strain of canine parainfluenza virus, and the deposit number is CGMCC No.19993. The canine parainfluenza virus attenuated strain of the invention has strong pertinence, can be used for dogs of various breeds and ages, and can also be used for pregnant dogs, with high antibody production and long immune period.

The invention discloses an I group 11d serotype fowl adenovirus strain, an inactivated vaccine and a preparation method of the inactivated vaccine. The I group 11d serotype fowl adenovirus strain is named as fowl adenovirus RC18, and the virus strain is preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC No. 17404. Experiments prove that the I group 11d serotype fowl adenovirus RC18 strain isolated by the invention has a high titer, strong proliferation ability, good immunogenicity, and matches with epidemic strains. an inactivated vaccine prepared from the I group 11d serotype fowl adenovirus RC18 strain is used to immunize experimental chickens, the experimental chickens can resist infection of the chicken flock caused by clinical epidemic I group fowl adenovirus (11d serotype), the immune response is generated fast, the attack protection rate is high, and no local or systemic adverse reactions caused by the vaccine do not occur, and the safety and stability are good. The invention provides an effective technical means for the prevention and treatment of chicken inclusion body hepatitis.

The invention belongs to the technical field of veterinary biological products, and in particular relates to a BHK-21 cell line BHK-21-BP-2 clone and its application in full suspension cell culture. The present invention provides BHK-21-BP-2 clone strain, which is preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee, and the preservation number is CGMCC No.17588. After the clone is inoculated with pseudorabies virus in full suspension cell culture, the cell changes rapidly, and the virus produced has a high virulence, and the virus can be harvested within 24h to 48h; The process is simple; the total protein content of the produced virus antigen solution is low, the obtained effective virus antigen has high integrity, the antigen quality is good, and has excellent immunogenicity and safety; the prepared pseudorabies virus vaccine has high stability and batch-to-batch The advantages of small difference, good immune effect and high safety.