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Method for preparing inactivated vaccine of porcine reproductive and respiratory syndrome

A technology for respiratory syndrome and inactivated vaccine, which is applied in the field of preparation of inactivated vaccine for porcine reproductive and respiratory syndrome, can solve problems such as biological safety hazards, and achieve the effects of high toxicity, enhanced immune response and good stability

Inactive Publication Date: 2009-12-23
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, many vaccines have been developed at home and abroad to prevent and control porcine reproductive and respiratory syndrome. However, due to the potential biosafety hazards of using attenuated porcine reproductive and respiratory syndrome vaccines to prevent porcine reproductive and respiratory syndrome, veterinary scientific and technological workers and Most production personnel advocate the use of inactivated vaccines to prevent

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1: a kind of preparation method of porcine reproductive and respiratory syndrome inactivated vaccine, its preparation steps are as follows:

[0019] ①. Preparation of virus seeds for production: Pour out the growth medium from the Marc-145 cells that have grown into a monolayer, so as to contain 0.01TCID 50 Each cell was inoculated with porcine reproductive and respiratory syndrome virus CQ strain virus liquid, after 37°C adsorption for 1 hour, DMEM maintenance solution containing 2% newborn bovine serum was added, and cultured and observed in 37°C containing 5% CO2 incubator for 48 hours, Freeze and thaw once to harvest infected cell venom and put it in a sterilized container.

[0020] ②. Preparation of virus liquid for seedling preparation: Pour out the growth liquid from the well-grown single-layer Marc-145 cells, and follow the 0.01TCID 50 / Cell inoculation with porcine reproductive and respiratory syndrome virus CQ strain production virus seed, after 3...

Embodiment 2

[0022] Embodiment 2: a kind of preparation method of porcine reproductive and respiratory syndrome inactivated vaccine, its preparation steps are as follows:

[0023] ①. Preparation of virus seeds for production: Pour out the growth medium from the Marc-145 cells that have grown into a monolayer, so as to contain 0.01TCID 50 Each cell was inoculated with porcine reproductive and respiratory syndrome virus CQ strain virus liquid, after 37°C adsorption for 1 hour, DMEM maintenance solution containing 2% newborn bovine serum was added, and cultured and observed in 37°C containing 5% CO2 incubator for 48 hours, Freeze and thaw once to harvest infected cell venom and put it in a sterilized container.

[0024] ②. Preparation of virus liquid for seedling preparation: Pour out the growth liquid from the well-grown single-layer Marc-145 cells, and follow the 0.01TCID 50 / Cell inoculation with porcine reproductive and respiratory syndrome virus CQ strain production virus seed, after 3...

Embodiment 3

[0026] Embodiment 3: a kind of preparation method of porcine reproductive and respiratory syndrome inactivated vaccine, its preparation steps are as follows:

[0027] ①. Preparation of virus seeds for production: Pour out the growth medium from the Marc-145 cells that have grown into a monolayer, so as to contain 0.01TCID 50 Each cell was inoculated with porcine reproductive and respiratory syndrome virus CQ strain virus liquid, after 37°C adsorption for 1 hour, DMEM maintenance solution containing 2% newborn bovine serum was added, and cultured and observed in 37°C containing 5% CO2 incubator for 48 hours, Freeze and thaw once to harvest infected cell venom and put it in a sterilized container.

[0028] ②. Preparation of virus solution for seedling production: Pour off the growth solution of the well-grown single-layer Marc-145 cells, and follow the 0.01TCID 50 / Cell inoculation with porcine reproductive and respiratory syndrome virus CQ strain production virus seed, after ...

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Abstract

The invention discloses a method for preparing an inactivated vaccine of porcine reproductive and respiratory syndrome, which comprises the following steps: (1) preparation of virus seed for production; (2) preparation of virus liquid for preparing vaccine; and (3) preparation of the inactivated vaccine which comprises: inactivating the virus liquid with the virus content more than or equal to 10TCID50 / ml at 4 DEG C for 48 hours; adding 94 to 98 volume portions of inactivated virus liquid into 2 to 6 volume portions of tween-80 and mixing to prepare aqueous phase; mixing 94 volume portions of white oil for injection and 6 volume portions of span-80 to prepare oil phase; mixing the aqueous phase and the oil phase in a mixing ratio of 1:1.5-1:2.5, emulsifying the mixture to prepare the inactivated vaccine; and sub-packaging and entering warehouse. The method has the advantages of simple production process, good effect of the prepared inactivated vaccine of the porcine reproductive and the respiratory syndrome and the like.

Description

Technical field: [0001] The invention belongs to the technical field of new veterinary biomedicine, and in particular relates to a preparation method of an inactivated porcine reproductive and respiratory syndrome vaccine. Background technique: [0002] Porcine reproductive and respiratory syndrome is a reproductive disorder and respiratory infectious disease of pigs caused by porcine reproductive and respiratory syndrome virus (PRRSV). It is widely distributed in the world, causing huge economic losses and seriously restricting pig breeding The healthy development of the industry has become a hot spot in swine disease research. Since there is no effective treatment for porcine reproductive and respiratory syndrome, vaccination is an important means of preventing and controlling the disease. At present, many vaccines have been developed at home and abroad to prevent and control porcine reproductive and respiratory syndrome. However, due to the potential biosafety risks of u...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/12A61P31/14A61P11/00A61P15/08
Inventor 孙进忠乔荣岑习向锋荣骏弓闵亚杰孟照国杨海岳晋春霞
Owner PU LIKE BIO ENG
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