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Duck virus hepatitis virus, duck virus hepatitis inactivated vaccine and preparation method of duck virus hepatitis inactivated vaccine

A duck viral hepatitis, inactivated vaccine technology, applied in antiviral immunoglobulin, biochemical equipment and methods, antiviral agents, etc., can solve the problem of unstable titer, unqualified duck embryo quality, duck embryo yolk antibody Production process hidden dangers and other problems, to achieve the effect of improving immune protection and facilitating full separation

Inactive Publication Date: 2014-06-04
北京中联康生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, there are many problems such as the unstable potency of duck viral hepatitis hyperimmune antibodies that have been marketed in China, duck embryos can vertically transmit certain homologous diseases, the quality of duck embryos is not up to standard, and there are hidden dangers in the production process of duck embryo egg yolk antibodies.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Duck Viral Hepatitis Virus

[0027] 1. Virus isolation

[0028] The liver was collected from a duck that was infected in a duck factory in Shandong, washed with sterilized normal saline, discarded more than the mucous membrane, cut into pieces, ground and mashed with sterilized equipment, and added 2 times the sterilized normal saline to make it blend with the tissue Mix, and then preliminarily filter with 100-mesh copper gauze, centrifuge at 3500rpm to get the supernatant, and finally sterilize it with a 0.22um filter membrane. Inoculate the allantoic cavity of 11-13-day-old SPF chicken embryos with virus extract, incubate at 37°C, discard dead chicken embryos within 48 hours, collect dead chicken embryos within 48-144 hours, collect allantoic fluid and amniotic fluid, Thus, duck viral hepatitis virus was isolated.

[0029] 2. Identification

[0030] Take the cases of suspected viral duck hepatitis that occurred in Shandong, after the tissue was homogenize...

Embodiment 2

[0031] Embodiment 2 prepares duck viral hepatitis inactivated vaccine

[0032] Using the allantoic fluid prepared in Example 1, 0.1% to 0.3% formaldehyde solution was added thereto, and airtightly inactivated at 37°C for 48 hours. After the inactivation is completed, prepare the oil phase part of the vaccine, the ratio of which is 94 parts of white oil, 806 parts of Siben, and 2 parts of aluminum stearate, heat and sterilize for later use; prepare the water phase part of the vaccine, the ratio of which is 96 parts of inactivated venom Parts, Tween 804 parts, so that it can fully dissolve completely. When emulsifying, the ratio of the water phase to the oil phase is 1:3, and the water-oil mixture is added to the emulsification machine, and emulsified to prepare the duck virus hepatitis inactivated vaccine.

Embodiment 3

[0033] Example 3 Antibody Preparation and Application

[0034] 1. The duck viral hepatitis inactivated vaccine antigen is prepared according to the method of Example 2, and is ready for use.

[0035] 2. Basic immunization: Inject the chickens to be immunized with the duck viral hepatitis inactivated vaccine antigen prepared above, specifically 0.5 ml per chicken subcutaneously in the neck.

[0036] 3. Intensive immunization: after 2 weeks of basic immunization, the second immunization is performed, specifically, 1.0ml per mouse is injected subcutaneously in the neck; the third enhanced immunization is performed after an interval of 2 weeks, specifically, 2.0ml is injected subcutaneously in the neck / bird; every 8 weeks thereafter, do a booster immunization, each neck subcutaneous injection of 2.0ml / bird.

[0037] 4. Collection of breeding eggs: 7 days after the second booster immunization, the egg yolks of high-free eggs were collected every three days, and the specific an...

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Abstract

The invention provides a duck virus hepatitis virus, a duck virus hepatitis inactivated vaccine and a preparation method of the duck virus hepatitis inactivated vaccine. The duck virus hepatitis virus is preserved in China General Microbiological Culture Collection Center with the preservation number of CGMCC No.7804. The duck virus hepatitis virus has strong toxicity, good immunogenicity, and high toxicity evaluation, and the vaccine prepared by the duck virus hepatitis virus has good safety and long immune protection period.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to duck viral hepatitis virus, duck viral hepatitis inactivated vaccine and a preparation method thereof. Background technique [0002] Duck viral hepatitis disease is an acute, septic, and contagious infectious disease that mainly affects ducklings. The disease mainly infects ducklings within 7 days of age. After the onset, it is an acute course. The incidence rate can reach 100%, and the mortality rate can be as high as 80-100%. Ducks over 10 days old were invisible infection, and the mortality rate decreased with increasing age. Duck viral hepatitis has caused huge economic losses to the duck industry, and has become the most harmful disease in the duck industry. [0003] The disease has outbreaks all over the world, and often causes large-scale epidemics, mainly harming ducklings within 20 days of age, and causing 100% of ducklings within 7 days of age to die. However, for duckli...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/00C07K16/10C07K16/02A61K39/29A61K39/42A61P31/14A61P1/16C12R1/93
Inventor 黄华张希娟刘延亭刘俊生郑杰贺云霞邓秋红
Owner 北京中联康生物科技股份有限公司
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