A kind of I group 11d type poultry adenovirus strain, inactivated vaccine and preparation method thereof
A technology of avian adenovirus and inactivated vaccine, which is applied in the field of inactivated vaccine and its preparation, group I 11d avian adenovirus strain, virus strain and inactivated vaccine, and can solve the problem that the strain cannot provide good protection and no cross protection. Insufficient prevention and control of vaccines, etc., to achieve the effects of rapid immune response, high safety, and increased virus titer titers
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Embodiment 1
[0037] Example 1 Isolation and identification of avian adenovirus RC18 strain
[0038] 1. Epidemiological investigation
[0039] Since 2017, the inventor has discovered a disease characterized by hepatomegaly and hemorrhage from some white-feathered broiler chickens and laying hens in Hebei and other areas of Shandong Province. After clinical investigation and laboratory testing, the initial diagnosis is inclusion bodies caused by group I adenovirus Hepatitis, so the liver, spleen, lung and other diseased tissues of chickens with typical symptoms were collected and stored in a -70°C refrigerator for later use.
[0040] 2. Virus isolation
[0041] After taking the liver of a dead chicken and grinding it up, add sterilized saline at a ratio of 1:5 to make a suspension; after repeated freezing and thawing three times, centrifuge at 12000r / min for 10min, and take the supernatant; add penicillin and streptomycin 10000IU / ml, filtered through a 0.22μm microporous filter, and stored...
Embodiment 2
[0056] Embodiment 2 uses SPF chicken embryo to prepare poultry adenovirus inactivated vaccine
[0057] The method for preparing poultry adenovirus inactivated vaccine according to the present invention covers the following steps: preparation of antigen for making vaccines, inspection of semi-finished products, preparation of vaccine, safety inspection and efficacy inspection, the specific steps are as follows:
[0058] 1. Preparation of antigen solution
[0059] 1.1 Incubation of eggs:
[0060] SPF chicken embryos (purchased from Beijing Meria Weitong Experimental Animal Technology Co., Ltd.) were selected and placed on 42 egg trays, and the air chamber must be placed upwards in the incubator for incubation.
[0061] 1.2 Virus inoculation and harvest:
[0062] The isolated virus species (avian adenovirus RC18) is properly diluted with sterilized physiological saline, press 10 3.0 ~10 4.0 ELD 50 / 0.1ml to inoculate 7-day-old chicken embryos via the chorioallantoic membrane...
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