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109 results about "Duck embryo" patented technology
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Balut ( /bəˈluː/ bə-LOOT, /ˈbɑːluː/ BAH-loot; also spelled as balot) is a developing bird embryo (usually a duck) that is boiled and eaten from the shell. It originated from and is commonly sold as street food in the Philippines. The Tagalog and Malay word balot means "wrapped".
Method for preparing duck virus hepatitis divalent refined egg yolk antibody
ActiveCN102827275AImprove securityHigh protection rateEgg immunoglobulinsImmunoglobulins against virusesDuck hepatitis A virusFreeze thawing
The invention relates to a method for preparing a duck virus hepatitis divalent refined egg yolk antibody. The method is that vaccines of a duck virus hepatitis virus 1 type YBH1 strain and novel YBHX strain with good immunogenicity are selected from duck virus hepatitis superior prevalent strains to prepare strains, the strains are respectively inoculated to a chicken embryo and a duck embryo, a dead embryo body and the embryo juice are respectively obtained, the virus liquid is collected after grinding and freeze thawing, ultrafiltration concentration and inactivation with formaldehyde solution are carried out, and oil adjuvant is added to mix and emulsify to produce the vaccine. The vaccine is inoculated to a laying hen, the egg with high immunity is obtained, the yolk is separated, inactivated, extracted and refined to produce the duck virus hepatitis divalent refined egg yolk antibody which can prevent the duck virus hepatitis which is caused by the 1 type and novel duck hepatitis viruses, so the egg yolk has the advantages of high efficiency, good safety, high protection ratio and the like.
Owner:YEBIO BIOENG OF QINGDAO
Method for processing sauced marinated duck
The invention relates to a method for processing a sauced marinated duck, and the method comprises the steps of taking a fresh duck or a duck embryo as a raw material, carrying out rapid salinization for a certain time by adopting the high pressure method, boiling for 20-30 minutes in marinade which is prepared by soy sauce, fine salt, liquor and white sugar, further boiling for 100-120 minutes in the marinade juice which is prepared by adding ginger, five spice powder, pepper and dried chilli in the marinade, fishing out, draining, cooling and then adopting UV irradiation for sterilization. The sauced marinated duck is pure gold, bright, fresh, tender and delicious, thereby being applicable to the requirements of scale production.
Owner:JIANGXI HUANGSHANGHUANG GROUP FOOD
DHAV (duck hepatitis A virus) JS strain and application of DHAV JS strain in duck virus hepatitis prevention and cure
ActiveCN103013931AReduce the injection doseReduce stress responseMicroorganism based processesAntiviralsDuck hepatitis A virusDisease
The invention discloses a novel DHAV (duck hepatitis A virus) JS strain and application of the DHAV JS strain in duck virus hepatitis prevention and cure. The DHAV JS strain is obtained through the steps of separation culture, duck embryo neutralization test, PCR (polymerase chain reaction) detection of viruses and sequencing detection experiment of the viruses, and the strain is determined to be the DHAV serotype 3; and the microbial preservation number is CGMCCNo.6852. According to the invention, through taking the novel DHAV JS strain as the virus strain for producing vaccine, the novel DHAV inactivated vaccine is prepared through the steps of inoculating 10-day-old SPF (specific pathogen free) duck embryos, selecting the embryos died after incubating for 48 hours, collecting the embryo liquid, inactivating and emulsifying. The clinical application proves that the prepared inactivated vaccine is favorable in safety and conducive to prevention and control of the novel DHAV disease, can prevent the infection and outbreak of the novel DHAV in a targeted way, and can be in mass production and clinical application.
Owner:哈药集团生物疫苗有限公司
Preparation and application of efficient cell inactivation vaccine against new duck reovirus disease
PendingCN105854011ARaise antibody levelsFight infectionViral antigen ingredientsAntiviralsDiseaseAntigen
The invention relates to preparation and application of an efficient cell inactivation vaccine against new duck reovirus disease. The preparation method comprises the following steps: an antigen preparation step: inoculating a virus NDRV-TH11 strain with immortalized duck embryo fibroblast to obtain cytotoxicity with 80% of cytopathic effect, and performing cryopreservation at -20 DEG C; and a vaccine preparation step: inactivating the obtained cytotoxicity and mixing with a homemade nano adjuvant and stirring uniformly to obtain the efficient cell inactivation vaccine against new duck reovirus disease. After the duck is immunized by the efficient cell inactivation vaccine against new duck reovirus disease prepared in the invention, the duck can effectively resist the attack of a virulent strain of the new duck reovirus.
Owner:SHANGHAI VETERINARY RES INST CHINESE ACAD OF AGRI SCI
Duck plague vaccines and special strains therefor
InactiveCN101200710AImprove securityEasy to useInactivation/attenuationAntiviralsActive componentVirus strain
The present invention discloses a duck plague vaccine and a special virus strain. The active component of the duck plague vaccine is inactivated duck plague bleb virus AV1221 strain duck embryo adapted virus. The duck plague vaccine of the present invention is made from inactivated virus, which has good safety and is convenient for storing, transporting (2 DEG C to 8 DEG C) and using, and the immunity effect is stable.
Owner:CHINA INST OF VETERINARY DRUG CONTROL
Method for preparing triple inactivated vaccine for preventing chicken Newcastle disease, infectious bronchitis and egg drop syndrome
InactiveCN101099863AImprove securityReduce the number of injectionsViral antigen ingredientsRespiratory disorderDiseaseInfectious bronchitis
The invention is concerned with a kind of preparation method of inactivated vaccine to prevent new castle disease, infectious bronchitis and egg drop syndrome vaccine. The selected genus is Newcastle Disease virus La Sota individual plant, infectious bronchitis virus M41 individual plant, HN99 kidney type of aberrance individual plant and EDS76 Jing 911 individual plant. Dilute three kinds of virus and inoculate in the allantoic cavity of SPF chick embryo and affectable duck embryo. Collect liquid of embryo to alive and dead embryos as seed. Dilute and inoculate in allantoic cavity of affectable chick embryo or duck embryo to get liquid of virus embryo. Concentrate with equipment and add with formaldehyde solution to prepare bacterin through emulsion process. This invention can prevent Newcastle Disease, chick kidney type and breathing type infectious bronchitis, and egg drop syndrome vaccine. It can reduce the times of injection and the cost of epidemic prevention for easy using and practicality.
Owner:HENAN AGRICULTURAL UNIVERSITY
Preparation methods for duck hepatitis virus immunogen and hyperimmune serum and application of duck hepatitis virus hyperimmune serum
InactiveCN104926939AThe preparation method requires low conditionsEasy to operateSerum immunoglobulinsImmunoglobulins against virusesDuck hepatitis A virusSerum ige
The invention provides preparation methods for duck hepatitis virus immunogen and hyperimmune serum and application of the duck hepatitis virus hyperimmune serum. According to the preparation methods and the application of the duck hepatitis virus hyperimmune serum, the duck hepatitis virus immunogen is obtained through inoculating a serum 1 type duck hepatitis virus CH60 strain DHAV-1 (Duck Hepatitis A Virus type 1) or a serum 3 type duck hepatitis virus CH1 strain DHAV-3 (Duck Hepatitis A Virus type 3) to an allantoic cavity of a chick embryo of 9-10 days old or a duck embryo of 10-12 days old and carrying out proliferation and treatment, and the hyperimmune serum is obtained through mixing the duck hepatitis virus immunogen with a Freund's complete adjuvant or Freund's incomplete adjuvant to prepare solutions of different concentrations, carrying out repeated immunization on immune animals and then sampling and collecting blood and can be applied to the diagnosis and detection on a duck hepatitis virus. The preparation methods provided by the invention have the advantages that the conditional requirements are low, the operation is simple, and the obtained immunogen can meet the requirements on the preparation of specific antiserum.
Owner:SICHUAN AGRI UNIV
Propolis injection for preventing duck hemorrhagic oophoritis and preparation method thereof
InactiveCN102228482AHigh protection rateExtended protection periodAnthropod material medical ingredientsViral antigen ingredientsDiseaseTembusu virus
The invention relates to the field of animal prevention medicine, particularly to a preparation method for an injection for preventing animal epidemic diseases from occurring, wherein the propolis injection for preventing duck hemorrhagic oophoritis is composed of a propolis aqueous solution, a duck embryo homogenated tissue inoculated with duck flavivirus-Tembusu virus and dead by infection, andan inactivation solution; the preparation method comprises the step of adding the inactivation solution in the mixed solution of the duck embryo homogenated tissue and propolis to obtain the propolisinjection for preventing duck hemorrhagic oophoritis; the propolis injection for preventing duck hemorrhagic oophoritis prepared by the preparation method of the invention has a remarkable effect to prevent duck hemorrhagic oophoritis (duck Tembusu virus disease); the protection ratio is increased to 90-100%, the protection period is prolonged to 6-12 months, the action time is shortened to 5 days, and only once injection can achieve an expected effect.
Owner:CHONGQING ACAD OF ANIMAL SCI
Serum type 3 duck hepatitis A virus live vaccine and preparation method thereof
InactiveCN104726414AInactivation/attenuationMicroorganism based processesDuck hepatitis A virusLiver tissue
The invention relates to a serum type 3 duck hepatitis A virus (DHAV-3) live vaccine and a preparation method thereof. The serum type 3 duck hepatitis A virus (DHAV-3) can only be separated and cultured by virtue of duck embryos generally at present, which creates a very big obstacle for the research of the virus and the research and manufacturing of a serum type 3 duck hepatitis live vaccine. According to the preparation method provided by the invention, a chick embryo yolk sac inoculation way is adopted, the serum type 3 duck hepatitis A virus is obtained by separating liver tissues of a duck with duck hepatitis, and an attenuated HuB60 strain (CGMCC No.10307) is obtained by virtue of chick embryo continuous passage culture, wherein the attenuated strain can be used for preparing the serum type 3 duck hepatitis A virus (DHAV-3) live vaccine, and can also be mixed with a serum type 1 duck hepatitis attenuated strain (such as A66 strain) according to an appropriate proportion to prepare a duck hepatitis A bivalent live vaccine.
Owner:兆丰华生物科技(南京)有限公司
Application of chicken liver cancer cell system as duck plague virus host
ActiveCN105420198APromote growthMicroorganism based processesDsDNA virusesCancer cellCell culture media
The invention belongs to the technical field of duck plague virus culturing, particularly relates to a new host cell of the duck plague virus, and discloses application of a chicken liver cancer cell system as a duck plague virus host. A traditional method for culturing the duck plague virus through the duck embryo fibroblast primary cell is limited by duck embryo supply and the hatching day age, and wastes time and labor during preparation. The established method for culturing the duck plague virus chicken liver cancer cell system is good in virus growth. The method for culturing the virus through a continuous cell line is not influenced by duck embryo supply, and has the advantages of being instantly available and capable of saving time and labor. The duck plague virus can grow and reproduce on a chicken liver cancer cell system continuous cell culture medium, and by means of a newly-found virus copying host cell, a new technical approach, method and material are provided for research of related fields.
Owner:JINLING INST OF TECH
Duck parvovirus strain and live vaccine thereof
ActiveCN105154410ASafe infectionInfection Prevention and ControlMicroorganism based processesAntiviralsDiseasePathogenicity
The invention relates to the field of animal virology and provides a duck parvovirus strain, live vaccine thereof and a preparing method. The strain is a duck parvovirus and specifically named GT2015v, and the biology preservation serial number of the strain is CCTCC No:V201528; the vaccine prepared by utilizing the strain effectively solves the problem that the disease can not be prevented at present in China, by means of continuous passage of duck embryos, a medium virulence isolate strain is attenuated and made to lose pathogenicity to ducks, good immunogenicity is kept, duck parvovirus infection can be prevented and controlled safely and effectively, cultivation risks are reduced, and sound development of duck breeding industry is guaranteed.
Owner:SHANDONG AGRICULTURAL UNIVERSITY
Quadruple inactivated vaccine for preventing chicken diseases
ActiveCN102166355AEasy to prepareGood effectAntiviralsAntibody medical ingredientsDiseaseInfectious bronchitis
The invention discloses a quadruple inactivated vaccine for preventing chicken diseases, which is prepared by respectively vaccinating Newcastle disease virus ZM10 strain, infectious bronchitis virus M41 strain and H9 subtype avian influenza virus S2 strain in a susceptible chicken embryo, and collecting an infected chicken embryo solution; vaccinating egg drop syndrome virus AV-127 strain in a susceptible duck embryo, and collecting an infected duck embryo solution; and concentrating the collected virus solutions, inactivating, blending with an oil adjuvant, and emulsifying. The quadruple vaccine provided by the invention can be used for preventing diseases caused by Newcastle disease viruses, infectious bronchitis viruses, egg drop syndrome viruses and avian influenza (H9 subtype) viruses to achieve the effect of preventing four diseases simultaneously and reduce immune cost and immune stress reaction.
Owner:乾元浩生物股份有限公司
Process for producing salted duck with soup juice
The invention provides a process for producing salted duck with soup juice, and relates to the technology field of foodstuff processing method, which is characterized in that the method comprises the following steps: cleaning and shaping, rubbing with salt for the first time and piling, dipping in sauce, rubbing with salt for the second time and piling, turning, cleaning duck embryo supporter, drying, hanging and packing. The soup with sauce for dipping comprises specially-produced ingredients. The product is marinated by pig sauce soup, and has the advantages of delicious taste, good mouthfeel, edible convenience, dry salting effect and long preservation time.
Owner:ANHUI WINS ONE MEAT FOOD
Duck tembusu virus envelop E protein inhibitory peptide and application thereof
InactiveCN104193805APrevent proliferationDownregulation of viral copy numberPeptide/protein ingredientsAnimal feeding stuffAnimal virusFeed additive
The invention discloses a duck tembusu virus envelop E protein inhibitory peptide and application thereof, belonging to the technical field of animal virus molecular biologics. DTMUV envelop E protein is obtained by using a genetic engineering method, DTMUV HN1 strain E protein is taken as a target, the duck tembusu virus envelop E protein inhibitory peptide is obtained through screening by using a bacteriophage random 12 peptide library, and the amino acid sequence of the duck tembusu virus envelop E protein inhibitory peptide is as shown in HWSTRQGSTRWN. By adopting the inhibitory peptide, proliferation of the duck tembusu virus in duck embryo fibroblast can be inhibited, the virus copy number of the DTMUV in the duck embryo fibroblast can be remarkably reduced in different concentrations, meanwhile the virus titer of the DTMUV in the duck embryo fibroblast can be remarkably reduced, and the DTMUV has no remarkable influence on proliferation of the duck embryo fibroblast. The duck tembusu virus envelop E protein inhibitory peptide has good application prospect in preparing medicines or feed additives for preventing the duck tembusu virus, and can be used for preventing and controlling the duck tembusu virus.
Owner:HENAN UNIV OF SCI & TECH
Boiled salted duck master sauce and preparation process thereof
InactiveCN101731557ALong lasting fragranceUnique fragranceFood preparationChinese cinnamonAdditive ingredient
The invention discloses boiled salted duck master sauce and a preparation process thereof, and the preparation process is as follows: as for the master sauce, ingredients such as star anise, Chinese cinnamon, clove and the like are added with water for decoction to prepare new sauce, duck embryo is added and marinated for five times to obtain bittern, and ingredients of which the dosage is half of that in preparing the new sauce are added and decocted after water is added to prepare the master sauce. The master sauce prepared in the invention has clear bittern and long and elegant flavor, and can effectively remove fishy smell of ducks; and the boiled saluted duck prepared by the master sauce has unique mellow flavor.
Owner:江苏迈斯克食品有限公司
Full suspension culture method of chicken infectious bursal disease virus
InactiveCN109295009AImprove adaptabilityHigh titerCulture processRecovery/purificationVirus CultivationVaccine Production
The invention discloses a full suspension culture method of chicken infectious bursal disease virus, wherein the method comprises the following steps: step 1, carrying out resuscitation and subcultureof subcultured cell lines derived from duck embryo cells; step 2, adopting a second-order culture method, inoculating the subcultured cell lines derived from the duck embryo cells subjected to full suspension culture with the chicken infectious bursal disease virus, and carrying out virus large-scale culture; and step 3, after inoculating with the virus, taking samples every other 12 h and measuring the virus TCID50, harvesting the virus and preserving when the virus TCID50 reaches the maximum, and thus obtaining the chicken infectious bursal disease virus after culture. The full suspension culture method improves the scale and efficiency of virus culture, conforms to the tendency of future vaccine production, has broad application prospects and contains good economic benefits.
Owner:ZHAOQING INST OF BIOTECHNOLOGY CO LTD +2
Duck embryo early sex identification method and special kit
ActiveCN104698173ADoes not affect normal developmentImprove welfareMaterial analysisDiseaseAnimal science
The invention belongs to the technical field of poultry sex detection, and in particular relates to a duck embryo early sex identification method and a special kit. The duck embryo early sex identification method is established by collecting a hatched early duck embryo allantoic fluid, making use of a monoclonal antibody for estradiol detection and carrying out antigen-antibody reaction. Male and female sexes are judged according to a characteristic that the content of estradiol in a female embryo is higher than 1ng / ml and the content of the estradiol in a male embryo is lower than 0.6ng / ml. The identification method disclosed by the invention can identify the female and male sexes in an early hatching period without an influence on the further development of the embryo; and the identification method is conducive to animal welfare and relieving the transmission of vertical diseases.
Owner:武汉赛维尔生物科技有限公司
Duck reovirus vaccine and preparation method thereof
ActiveCN109718370AClear cell backgroundClear backgroundViral antigen ingredientsAntiviralsAntigenPrimary cell
The invention discloses a duck reovirus vaccine. The inactivated vaccine is obtained by inoculating duck reovirus virus seed on continuous cell line BHK-21 cells. A Reed-Muench method is used to determine that the virus titer can reach 107.0-107.5 TCID50 / 0.1mL. The stable production and high titer of antigen are the most important factors in the preparation of vaccines. The duck reovirus proliferation is carried out by using BHK-21 cells, so that the virus titer is 10 times or above as high as that of a conventional duck embryo method and a primary cell method. If the virus proliferation is carried out by using a bioreactor, the virus titer can reach 100-time level. The duck reovirus inactivated vaccine disclosed by the invention has the advantages of high yield, stable quality, good immune effect and huge application prospect, and immune ducks can generate a high-level serum neutralizing antibody.
Owner:广东渔跃生物技术有限公司
Muscovy duck parvovirus and gosling plague bivalent vaccine
ActiveCN105920596AImproving immunogenicityImprove securityViral antigen ingredientsAntiviralsMaternal antibodyImmunogenicity
The invention provides a Muscovy duck parvovirus and gosling plague bivalent vaccine. The antigens used by the vaccine is inactivated Muscovy duck parvoviruses and Muscovy duck-source gosling plague viruses, the preservation number of the Muscovy duck parvoviruses is CGMCC No. 8504, and the preservation number of the Muscovy duck-source gosling plague viruses is CCTCC No. V201620. A preparation method of the Muscovy duck parvovirus and gosling plague bivalent vaccine includes: the Muscovy duck parvovirus YBMDP strains and Muscovy duck-source gosling plague virus YBGPV-M strains which are high in virus content and good in immunogenicity are screened, infected embryos and allantoic fluid are collected after duck embryo inoculation, and oil emulsion adjuvant is added for emulsification and mixing to obtain the vaccine after homogenization, ultrafiltration and concentration, and formaldehyde solution inactivation. The prepared vaccine can immunize breeding Muscovy ducks and increase the level of two types of antibodies of the breeding Muscovy ducks at the same time, guarantee the offspring maternal antibody level of the breeding Muscovy ducks, and prevent the young Muscovy duck parvovirus diseases caused by the Muscovy duck parvoviruses and gosling plague virus infection caused by the Muscovy duck-source gosling plague viruses.
Owner:YEBIO BIOENG OF QINGDAO
Preparation method of divalent inactivated vaccines for duck virus hepatitis
ActiveCN102908618APrevention of Duck Viral HepatitisAntiviralsAntibody medical ingredientsFreeze thawingDuck hepatitis A virus
The invention relates to a preparation method of divalent inactivated vaccines for duck virus hepatitis. According to the invention, the preparation method comprises the following steps of: screening duck virus hepatitis type I YBH1 strains and novel YBHX strain vaccines with good immunogenicity from preponderant popular strains so as to prepare strains, respectively inoculating the vaccines on a chicken embryo and a duck embryo, respectively harvesting embryoid bodies and embryoid fluid of dead embryos, collecting a viral solution after the embryoid bodies and the embryoid fluid are ground and frozen-thawed, and adding an oil adjuvant for mixing and emulsifying to obtain the vaccines after the viral solution is carried out ultrafiltration and concentration and is inactivated through a formaldehyde solution. The vaccines can carry out immunization for animals and can simultaneously prevent the duck virus hepatitis caused by type I duck virus hepatitis and novel duck virus hepatitis, and the divalent inactivated vaccines for the duck virus hepatitis have the advantages of high efficiency, good safety and high protective ratio and the like.
Owner:YEBIO BIOENG OF QINGDAO
Duck astrovirus vaccine and preparation method thereof
ActiveCN109692329AClear cell backgroundClear backgroundSsRNA viruses positive-senseViral antigen ingredientsAntigenImmune effects
The present invention discloses a duck astrovirus vaccine which is an inactivated vaccine obtained by inoculating duck astrovirus type I and type III virus seeds on a continuous cell line BHK-21 cell.The duck astrovirus type I and type III are propagated by the BHK-21 passage cell, and the virus titer is stable to 106.5-107.0TCID50 / 0.1mL by a Reed-Muench method. The stable production and high titer of antigen are the most important factors in the preparation of the vaccine, the virus titer of duck astrovirus type I and type III proliferation with the BHK-21 cell is more than 10 times that ofa conventional duck embryo method and a primary cell method. If a bioreactor is used for virus proliferation, the virus titer can reach 100 times. The duck astrovirus (type I and III) inactivated vaccine disclosed in the invention has high yield and stable quality, and the immune duck can produce a high-level serum neutralizing antibody, the immune effect is good, and the application prospect is great.
Owner:广东渔跃生物技术有限公司
Bivalent egg yolk antibody against DVH (duck virus hepatitis) as well as preparation method and application of bivalent egg yolk antibody
ActiveCN106854647AImprove securityEffective separation and purificationEgg immunoglobulinsSsRNA viruses positive-senseDuck hepatitis A virusYolk
Owner:PU LIKE BIO ENG
Cherry valley duck embryo epithelial cell line and establishment method thereof
ActiveCN103725644ALow nutritional requirementsReduce the cost of trainingEmbryonic cellsSurvivabilityCulture fluid
The invention discloses a cherry valley duck embryo epithelial cell line and an establishment method thereof, and belongs to the field of cytobiology. The method comprises the steps of taking duck embryo tissue with 9 to 12 age in days in a sterile way, shearing the duck embryo tissue into tissue blocks, adhering the tissue blocks downwards to the bottom of a culture hole, adding nutrient solution into a carbon dioxide incubator, and performing plate adhering culture at 37 DEG C. The high-survivability and high-purity cherry valley duck embryo epithelial cell line is obtained according to the method and conducted to biological preservation, compared with primary cell, the cell line has the advantages that the cellular morphology and the physiological property of the cell line are obvious different from that of the primary cell, continuous and stable passage can be realized, the nutritional requirement is low, the growth cycle is short, and the cell line is suitable for large-scale culture, provides a large amount of high-quality cell material for life sciences, such as gene engineering, cell engineering, immunology, molecular biology and the like, can serve as donor cell for poultry somatic cell clone breeding, and can also serve as a main material for duck virus isolation and vaccine production.
Owner:SHANDONG LVDU BIO SICIENCE & TECH
B-cell epitope of VP(viral protein)3 of DHAV (duck hepatitis A virus)-1 as well as identification method and application of B-cell epitope
ActiveCN105198969AEasy to identifyRapid identificationSsRNA viruses positive-senseViral antigen ingredientsDuck hepatitis A virusElisa method
Owner:SICHUAN AGRI UNIV
Duck viral hepatitis bivalent yolk antibody, preparation method and application thereof
InactiveCN103865884AImprove securityEffective separation and purificationEgg immunoglobulinsSsRNA viruses positive-senseYolkOctanoic Acids
The present invention provides a duck viral hepatitis bivalent yolk antibody and a preparation method thereof, wherein the bivalent yolk antibody comprises a duck viral hepatitis DHAV-1 type antibody and a duck viral hepatitis DHAV-3 type antibody. The preparation method comprises: (1) adopting a duck viral hepatitis DHAV-1 type strain and a duck viral hepatitis DHAV-3 type strain to respectively vaccinate SPF chicken embryo and susceptible duck embryo, harvesting allantoic fluid, mixing the harvested virus liquids according to a certain ratio, carrying out formaldehyde inactivation, and preparing a vaccine; (2) adopting the vaccine to immunize laying hens, sampling after immunization to determine whether the neutralizing titer of the anti-DHAV-1 type antigen antibody and the anti-DHAV-3 type antigen antibody in the chicken hyperimmune egg yolk is more than or equal to 1:8192, and collecting the hyperimmune egg of the chicken; and (3) disinfecting the eggshell of the hyperimmune egg, collecting the egg yolk, adding the equal volume of distilled water, uniformly stirring and mixing, carrying out low temperature pasteurization inactivation, adopting an acidification distilled water method to purify, adopting an octanoic acid method to purify, and carrying out micro-filtration and ultra-filtration. The duck viral hepatitis bivalent yolk antibody has characteristics of low cost and high titer, and can be provided for effectively controlling duck viral hepatitis caused by DHAV-1 and DHAV-3 so as to obtain significant social benefits.
Owner:PU LIKE BIO ENG
Complex duck interferon-alpha gene, and recombinant vector and application thereof
The invention discloses a complex duck interferon-alpha gene, and a recombinant vector and application thereof. The nucleotide sequence of the complex duck interferon-alpha gene is disclosed as SEQ ID NO:1. The optimized complex duck interferon-alpha gene can be expressed in Pichia yeast gene engineering bacteria to produce complex duck interferon-alpha. The obtained complex duck interferon-alpha has the following advantages: high purity: the thin-layer chromatography scanning on the yeast-expressed duck interferon-alpha SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) result indicates that the target ribbons of the recombinant yeast duck interferon-alpha account for more than 80% of the total expression proteins; and high antivirus action: compared with the yeast-expressed natural duck interferon-alpha, the protein expressed by the novel genome has higher antivirus activity, and the action of resisting 100TCID50VSV virus infection on duck embryo fibroblasts is enhanced by 40 times. The complex duck interferon-alpha gene can be used for preparing drugs for treating poultry virus diseases.
Owner:广东省农业科学院兽医研究所
Roasting method of frozen duck embryos
The invention relates to the field of roasting of meat products, and in particular relates to a roasting method of frozen duck embryos. The roasting method is characterized by comprising the following steps: 1, cleaning the duck embryos, filling a material into the duck embryos after first inflation, burning the skin of the duck embryos and scattering sugar on the duck embryos, air-drying and freezing in a refrigerator after the secondary inflation; 2, thawing the frozen duck embryos in an air-cooled refrigerator for 8-10 hours; 3, placing the thawed duck embryos in a far infrared oven, roasting at 170 DEG C for 40-45 minutes, roasting at 190 DEG C for 8 minutes and discharging when the temperature in the center of the duck embryos reaches 80 DEG C. According to the method, the processing conditions are accurately controlled, the mouthfeel is improved and the product quality is guaranteed on the premise of guaranteeing the safety and the sanitation during earlier stage processing, freezing and roasting of the duck embryos.
Owner:NANNING XINSHIJI FOOD
Motherwort extract and preparation method thereof
InactiveCN106974960APromote recoveryAnthropod material medical ingredientsAerosol deliveryMotherwortMedicine
Owner:广西山水牛畜牧业有限责任公司
Oocyte separation and extraction method based on duck embryo
InactiveCN107164316AReduce pollutionEfficient acquisitionCell dissociation methodsGerm cellsPhosphateDigestion Treatment
The invention discloses an oocyte separation and extraction method based on a duck embryo. The method comprises the following steps: selecting ovary tissue of a duck embryo, putting the ovary tissue into a sterilized PBS (Phosphate Buffer), and cutting the ovary tissue; adding a mixed enzyme digestive solution into the cut ovary tissue, putting ovary tissue fragments and the mixed enzyme digestive solution into a small-mouth digestive bottle, and performing digestion treatment on a shaking table; beating the ovary tissue fragments by using a pipette, adding fetal calf serum to terminate digestion, and filtering by using a cell screening net; collecting filtrate, and performing centrifugal treatment; abandoning the filtrate, adding a cell culture medium into precipitated cells, gently beating by using the pipette to suspend the cells, and laying a cell suspension on a culture dish; performing separation and extraction, so as to obtain oocyte based on the duck embryo. By adopting the method, grown livestock does not need to be dissected, cell contamination is reduced, the testing cost is reduced, and the oocyte can be rapidly and efficiently acquired.
Owner:ANIMAL SCI RES INST GUANGDONG ACADEMY OF AGRI SCI
A method for fully suspension culture of duck plague virus
InactiveCN109207439AImprove adaptabilityHigh titerMicroorganism based processesDsDNA virusesVaccine ProductionEconomic benefits
The invention discloses a full suspension culture method of a duck plague virus, comprising the following steps: step 1: resuscitation and passage culture of passage cell lines derived from duck embryo cells; 2, inoculation of the duck plague virus into a passage cell line derived from a fully suspend culture duck embryo cell by adopting a second-order culture method to realize large-scale cultureof that duck plague virus. 3, after that virus is inoculated, the TCID50 of the virus is sampled and detected every 12 hours, the virus is harvested when the TCID50 of the virus reach the maximum, and the virus is stored to obtain the cultured duck plague virus. The full suspension culture method of the invention improves virus culture scale and efficiency, accords with the trend of vaccine production in the future, and has wide application prospect and good economic benefit.
Owner:ZHAOQING INST OF BIOTECHNOLOGY CO LTD +3
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