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Serum type 3 duck hepatitis A virus live vaccine and preparation method thereof

A duck hepatitis A and virus technology, applied in the field of serum type 3 duck hepatitis A live vaccine and its preparation, can solve problems such as restricting duck production

Inactive Publication Date: 2015-06-24
兆丰华生物科技(南京)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] As we all know, my country is a big duck raising country. At present, the annual duck breeding capacity has reached 4 billion, accounting for more than 70% of the total duck breeding in the world. Duck hepatitis has always been the number one disease in duck production in my country. In recent years, with the increase of serum 1 The popularization and application of type duck hepatitis A live vaccine and high immune yolk antibody have effectively controlled duck hepatitis A serum type 1. The spread has become a new serious problem restricting duck production in my country

Method used

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  • Serum type 3 duck hepatitis A virus live vaccine and preparation method thereof
  • Serum type 3 duck hepatitis A virus live vaccine and preparation method thereof
  • Serum type 3 duck hepatitis A virus live vaccine and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0080] ——Preparation of DHAV-3 attenuated live vaccine (HuB60 strain)

[0081] 1. Antigen Preparation

[0082] (1) Seed poison DHAV-3 attenuated HuB60 strain F60, F65, F70, F75, F80 generation seed poison, 8-day-old SPF chicken embryo, etc.;

[0083] (2) Chicken embryo inoculation and antigen treatment

[0084] Qualified DHAV-3 attenuated HuB60 strain F60, F65, F70, F75, F80 generation seeds were diluted 100-1000 times with sterilized normal saline, and inoculated with 8-day-old SPF chicken embryos through the yolk sac route, with 0.2 ml, continue incubation at 36-37°C, discard dead embryos within 24 hours, harvest dead chicken embryo tissues (fetus, embryo membrane) within 24-96 hours, homogenize in groups, retain samples and freeze them.

[0085] (3) Antigen test Sterility test and virus content determination were carried out on the homogenate samples of chicken embryos in each group.

[0086] (4) Antigen preparation and test results, see Table 5 for details.

[0087] Ta...

Embodiment 2

[0104] ——Preparation of duck hepatitis A bivalent live vaccine (DHAV-1A66 strain, DHAV-3HuB60 strain)

[0105] 1. Antigen Preparation

[0106] (1) Seed virus duck hepatitis virus (duck hepatitis virus) DHAV-1A66 strain E66, E70, F72, E75, E80 generation seed virus This virus strain is the duck hepatitis virus (duck hepatitis virus) that the inventor isolated from my country's Anhui in 1986 Hepatitis virus) strain A was weakened by continuous passage of SPF chicken embryos. This strain of duck hepatitis virus (duck hepatitis virus) was sent to No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing, Chinese Academy of Sciences on August 13, 2013 The Institute of Microbiology and the General Microbiology Center of the China Committee for the Collection of Microbial Cultures have preserved it with the preservation number CGMCC No.8020.

[0107] (2) Chicken embryo inoculation and antigen treatment

[0108] Qualified DHAV-1A66 strains E66, E70, F72, E75, and E80 were diluted 10...

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Abstract

The invention relates to a serum type 3 duck hepatitis A virus (DHAV-3) live vaccine and a preparation method thereof. The serum type 3 duck hepatitis A virus (DHAV-3) can only be separated and cultured by virtue of duck embryos generally at present, which creates a very big obstacle for the research of the virus and the research and manufacturing of a serum type 3 duck hepatitis live vaccine. According to the preparation method provided by the invention, a chick embryo yolk sac inoculation way is adopted, the serum type 3 duck hepatitis A virus is obtained by separating liver tissues of a duck with duck hepatitis, and an attenuated HuB60 strain (CGMCC No.10307) is obtained by virtue of chick embryo continuous passage culture, wherein the attenuated strain can be used for preparing the serum type 3 duck hepatitis A virus (DHAV-3) live vaccine, and can also be mixed with a serum type 1 duck hepatitis attenuated strain (such as A66 strain) according to an appropriate proportion to prepare a duck hepatitis A bivalent live vaccine.

Description

technical field [0001] The invention relates to a live serum type 3 duck hepatitis A vaccine (DHAV-3) and a preparation method thereof, belonging to the field of veterinary biological products. Background technique [0002] Duck hepatitis A virus (duck hepatitis A virus, DHAV) is a member of Picornaviridae (Lu Chengping. Veterinary Microbiology. (Fifth Edition) Beijing: China Agricultural Press, 2012, 460-466.), is the cause of The most important pathogen of duck hepatitis mainly infects ducklings under 4 weeks of age with acute hepatitis characterized by hepatic hemorrhage. Since duck hepatitis caused by DHAV was discovered in the U.S. in the 1950s, it broke out and spread rapidly in the world's duck-raising areas. my country's Huang Junjian (Huang Junjian. Duckling Viral Hepatitis Research, Shanghai Academy of Agricultural Sciences Animal Husbandry and Veterinary Research Institute, 1963 .) In 1963, the outbreak of the disease in duck farms in Shanghai was first reported. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/00C12N7/08A61K39/125A61P31/14C12R1/93
Inventor 张小飞卢凤英丁美娟黄显明李华东
Owner 兆丰华生物科技(南京)有限公司
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