185 results about "Hepatitis A" patented technology

A composition comprising an isolated fowl adenovirus (FAdV), wherein the FAdV is a strain selected from FAdV-2, FAdV-7, FAdv-8a, FAdV-8b, FAdV-8a/8b or FAdV-11 serotype strains; and a suitable carrier and methods for inducing protective immunity in a subject and/or its progeny.

A non-washing disinfecting gel for disinfecting human skin and surface of object is prepared from triclosan, alcohol, moistening agent, thickening agent, pH regulator and deionized water through dissolving triclosan in alcohol, and sequentially adding others while stirring. It can be used to prevent SARS, hepatitis A, hepatitis B, and AIDS.

The present invention provides a freeze-dried hepatitis-A-chicken-pox attenuation combined vaccine and a production thereof. The combined vaccine has the security and the immunogenicity as good as the univalent vaccine and can effectively prevent the hepatitis A and the chicken pox. Two kinds of diseases can be effectively prevented just by one immunization, which can reduce vaccine cost, improve vaccine inoculation efficiency and reduce the bad reaction caused by a plurality of times of injections, and can reduce the vaccine inoculation times but realize the purpose of preventing various diseases.

The invention provides a fluorescence quantitative RT-PCR detecting kit for an enterovirus and a detecting method thereof; and the sequences of an upstream primer and a downstream primer and a specific probe of the fluorescence quantitative RT-PCR detecting kit are as follows: the upstream primer EV(YG)F is 5'-GGCTGCGYTGGCGGCC-3', the downstream primer EV(YG)R is 5'-CCAAAGTAGT CGGTTCCGC-3' and the specific probe EV(YG)PB is 5'-CTCCGGCCCCTGAATGCGG-3'. The method has high specificity on detecting the enterovirus and does not have cross reactions with other enteroviruses such as hepatitis A, hives, rubella, parotitis, encephalitis, dengue fever, adenovirus, and the like. The detection sensitivity of the method achieves 0.1TCID50; the method can directly detect the nucleic acid of the enterovirus from the samples of ncurolymph, herpes liquid, dejecta and the like of suspected patients; only about 3h is needed from extracting the nucleic acid of the enterovirus to finishing the detection; and the detecting kit and the detecting method are suitable for the lab early diagnosis of sudden epidemic caused by the infection of the enteroviruses such as the hand-foot-and-mouth disease and the like.

The invention provides a method of preparing a hepatitis A inactivated vaccine, which comprises the following steps of inoculating mixed and absorbed hepatitis A virus strain SH and a human embryo diploid cell MRC-5 to hepatitis A virus propagated in a cell factory, digesting the cell in the virus propagation peak to obtain cell virus liquid, removing impurity proteins of the cell by ultrasonication, chloroform extraction and ultrafiltration through ultrafiltration membranes, degerming and filtering by gel filtration chromatography and purification, and absorbing by an aluminium hydroxide adjuvant so that the hepatitis A inactivated vaccine is prepared. The result of in vivo effectiveness experiments performed on a mouse shows that the hepatitis A inactivated vaccine prepared by the method of the invention has higher ED50 and better immunogenicity than the contract strain. The method of the invention can improve the safety of the vaccine, simplify the technique, shorten the productionperiod, reduce the production cost, and realize the scale production of the hepatitis A inactivated vaccine.

The invention relates to a serum type 3 duck hepatitis A virus (DHAV-3) live vaccine and a preparation method thereof. The serum type 3 duck hepatitis A virus (DHAV-3) can only be separated and cultured by virtue of duck embryos generally at present, which creates a very big obstacle for the research of the virus and the research and manufacturing of a serum type 3 duck hepatitis live vaccine. According to the preparation method provided by the invention, a chick embryo yolk sac inoculation way is adopted, the serum type 3 duck hepatitis A virus is obtained by separating liver tissues of a duck with duck hepatitis, and an attenuated HuB60 strain (CGMCC No.10307) is obtained by virtue of chick embryo continuous passage culture, wherein the attenuated strain can be used for preparing the serum type 3 duck hepatitis A virus (DHAV-3) live vaccine, and can also be mixed with a serum type 1 duck hepatitis attenuated strain (such as A66 strain) according to an appropriate proportion to prepare a duck hepatitis A bivalent live vaccine.

A traditional Chinese medicine for curing acute hepatitis with jaundice belongs to a traditional Chinese medicine for curing hepatitis. The components contain: 25g of virgate wormwood herb, 15g of tuckahoe, 15g of polyporus, 15g of oriental waterplantain rhizome, 15g of largehead atractylodes rhizome, 15g of chinese thorowax root, 15g of turmeric root tuber, 30g of danshen root; the invention has the auxiliary prescriptions that: for severe heat, 20g of cape jasmine fruit, 20g of rhubarb and 20g of atrina glass are added; for severe dampness, 20g of agastache rugosa, 20g of atractylodes rhizome and 20g of roud cardamon seed are added; for static blood, 25g of red peony root, 25g of tree peony bark and 25g of peach seed are added. The substances in the prescription are made from natural traditional Chinese medicine plus traditional preparing methods. The materials are easy to collect, the prescription and the preparation methods are simple, and the cost of medicine preparation is low. The compatibility of the traditional Chinese medicine is simple; the medicine used in the prescription is fully natural herbal medicine, which is easy to collect and use; the preparation method is simple; the effects for taking and curing are good; the cost of the medicine is low, and the medicine is in particular suitable for the people living in remote villages far from towns; the cost is low for curing the people suffering from acute hepatitis with jaundice, which solves the problem that the local area lacks medical conditions to cure the disease because of low household income and poor living condition.

The invention includes following steps: computer controlled testing of enzyme marking device; receiving result data tested by the device; recognizing OD value; saving data of tested result data to file; under region of gray scale set, determining negative or positive and saving them to database; storing quality control value and comparison value to database. The invention provides following functions: displaying all results of samples at a time; checking tested results; maintaining results and printing out tested report form including negative or positive and OD value of each sample at one time. Comparing with current method, the invention possesses features of simple operation, saving half time and labor, reflecting degree of state of an illness and infection. The invention provides online searching and real time quality control to ensure tested report in accuracy, quick and fast transmission. The method is also applicable to hepatitis A, C, D, and E.

The present invention relates to a new hepatitis A virus strain YN5, and method for preparing hepatitis A inactivated vaccine by using said virus strain YB5 and Vero cell and the vaccine made up by using said method. Said invention also relates to the method for breeding said virus strain.

A kind of inartificial medicine Rabdosia.serra (Maxim) Hara extraction materials which may cure hepatitis and its preparation method is disclosed in the invention, which acts Rabdosia.serra (Maxim) Hara as materials, adopts one of the hot water, supercriticality CO2 or organic impregnant extracting methods, and executes Rabdosia.serra (Maxim) Hara extracting materials by decompression condensation, vacuum dryness, spray dryness etc. methods. It contains antivirus active component 2-hydroxyl ursocic acid, 2,3-di hydroxyl ursocic acid and antioxygenation active component rosmarinic acid and so on speciality valid components, and so it has restraining HBV copy, protecting immunity liver damnification, advancing cell immunity and increasing spleen, thymus weight, bile drainage of low immunity experiment animals etc. functions.

A Chinese medicine for treating hepatitides and hepatic disfunction caused by hepatitis and protecting liver is prepared from 8 Chinese-medicinal materials including giant knotweed rhizome, lysimachia, gymstemma pentaphyllum, astragalus root, etc.

The invention pertains to the field of Chinese traditional medicine, in particular to a traditional Chinese medicine used for treating hepatopathy and a preparation method thereof. The traditional Chinese medicine is made of the following bulk drugs: capillary wormwood herb, giant knotweed, spreading hedyotis herb, red sage root, red peony root, nutagrass flatsedge rhizome, wolfberry fruit and ginseng. The traditional Chinese medicine has the advantages of simple formula, definite treatment effect, few toxic and side effects as will as high cure rate and quick effect. The cure rate of the traditional medicine is over 98% for common hepatitis A and over 80% for hepatitis B and other hepatic diseases.

A screening method of identifying a compound for treating hepatitis. Also disclosed is a method for evaluating responsiveness of a subject having hepatitis to a drug.

A Chinese medicine for treating hepatitis is prepared from 34 Chinese-medicinal materials including evodia fruit, oriental wormwood, liquorice root, dandelion herb, etc.

A auxiliary therapeutic agent for hepatitis C combined treating with a complex therapeutic method (interferon and Ribavirin)for hepatitis C, which is composed of (by weight) 50-90 part of Chinese caterpillar fungus and 10-50 part of astragalus. Said invention can adjust immune function of patient and improve curative effect for hepatitis C.

The invention discloses a group of protein marker for simultaneously detecting the specificity of hepatitis A, hepatitis B, hepatitis C, Aids, syphilis and tuberculosis in blood serum, a kit for detecting the group of specific proteins and application thereof. The protein marker for detection the specificity in the invention comprises seven proteins with different molecular weights in a serum proteome map: A: 5232.13, B: 8784.25, C: 2017.5, D: 4309.19, E 5489.97, F: 8076.62 and G: 2155.92. A detection method for the specific proteins comprises the steps of: processing a serum sample to be detected by using a lysis solution; incubating and capturing serum proteins by using a nano magnetic bead with carboxyl as a genin; and eluting the nano magnetic bead, and detecting seven specific proteins in eluted ingredients by using a protein reader. The kit and the detection method in the invention have the advantages of rapidness, sensitivity, specificity, flexibility, simplicity for operation and the like.