Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Fluorescent quantitative RT-PCR detection kit and detection method for enterovirus

A detection kit, RT-PCR technology, used in fluorescence/phosphorescence, microbial determination/inspection, biochemical equipment and methods, etc., can solve the problems of inability to identify antigenic variants or new types, time-consuming and cumbersome

Inactive Publication Date: 2009-04-15
ZHEJIANG CENT FOR DISEASE CONTROL & PREVENTION
View PDF0 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

With the eradication of poliovirus, some new infectious diseases caused by enteroviruses are also popular from time to time, so the laboratory diagnosis of enteroviruses is becoming more and more important, but the traditional serum neutralization test is not only tedious, time-consuming, but also It is impossible to identify all serotypes, let alone some antigen variants or new types

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Fluorescent quantitative RT-PCR detection kit and detection method for enterovirus
  • Fluorescent quantitative RT-PCR detection kit and detection method for enterovirus
  • Fluorescent quantitative RT-PCR detection kit and detection method for enterovirus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] 1 Materials and methods

[0044]1.1 Virus strains and clinical specimens:

[0045] Enterovirus EV71, Coxsackie group A (CA) CA4, CA16, CA21, Coxsackie B5, E6, E30, POLIO type 1, POLIO type 3 and other virus strains were obtained from Chinese Academy of Medical Sciences, Beijing Institute of Biological Products and isolates from Zhejiang Provincial Center for Disease Control and Prevention. The clinical samples come from the cerebrospinal fluid, herpes fluid, feces, etc. of patients suspected of the recent enterovirus outbreak in Zhejiang Province. After the samples are collected, they are transported to the laboratory with ice.

[0046] 1.2 Primers and probes

[0047] Enterovirus strains from all over the world were downloaded from the NCBI gene bank in the United States, including all 67 serotypes of enteroviruses. The homology comparison was carried out, and primers and Taqman probes were designed in the non-coding general conserved gene region at the 5′ end of all...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a fluorescence quantitative RT-PCR detecting kit for an enterovirus and a detecting method thereof; and the sequences of an upstream primer and a downstream primer and a specific probe of the fluorescence quantitative RT-PCR detecting kit are as follows: the upstream primer EV(YG)F is 5'-GGCTGCGYTGGCGGCC-3', the downstream primer EV(YG)R is 5'-CCAAAGTAGT CGGTTCCGC-3' and the specific probe EV(YG)PB is 5'-CTCCGGCCCCTGAATGCGG-3'. The method has high specificity on detecting the enterovirus and does not have cross reactions with other enteroviruses such as hepatitis A, hives, rubella, parotitis, encephalitis, dengue fever, adenovirus, and the like. The detection sensitivity of the method achieves 0.1TCID50; the method can directly detect the nucleic acid of the enterovirus from the samples of ncurolymph, herpes liquid, dejecta and the like of suspected patients; only about 3h is needed from extracting the nucleic acid of the enterovirus to finishing the detection; and the detecting kit and the detecting method are suitable for the lab early diagnosis of sudden epidemic caused by the infection of the enteroviruses such as the hand-foot-and-mouth disease and the like.

Description

(1) Technical field [0001] The invention relates to an enterovirus nucleic acid fluorescence quantitative RT-PCR detection kit and a detection method, which can be applied to laboratory emergency detection of outbreaks caused by enteroviruses. (2) Background technology [0002] Enteroviruses (EVs) belong to the picornavirus family and can be divided into polioviruses, coxsackieviruses, echoviruses and enteroviruses 68-71 according to serology, with a total of 67 serotypes. Enteroviruses can cause many diseases, and can break out and cause death, seriously endangering human health. With the eradication of poliovirus, some new infectious diseases caused by enteroviruses are also popular from time to time, so the laboratory diagnosis of enteroviruses is becoming more and more important, but the traditional serum neutralization test is not only tedious, time-consuming, but also It is impossible to identify all serotypes, let alone some antigen variants or new types. The fluore...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/70C12Q1/68G01N21/64
CPCY02A50/30
Inventor 卢亦愚严菊英徐昌平冯燕陈寅
Owner ZHEJIANG CENT FOR DISEASE CONTROL & PREVENTION
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com